Antibacterial Activity of Phenyllactic acid Against Staphylococcus Epidermidis and Its Microbial Production: Modelling and Optimization-Based Analysis
-
Jianxiong Ye
,
Jianzhong Huang
Abstract
Phenyllactic acid (PLA), an organic acid with extensive antimicrobial activity, is considered as a promising natural preservative to replace chemical preservatives. In order to study the inhibitory pattern of PLA, this paper established a novel mathematical model for the growth of Staphylococcus epidermidis under PLA inhibition. The simulated results showed that the relationship between the antimicrobial activity of PLA against S. epidermidis and its concentration was suitable to be represented by an exponential function. Based on the proposed model, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of PLA against S. epidermidis were evaluated. The computed results were found to match experimental data. The MBC value was found to be independent of the initial biomass of S. epidermidis from both the simulated results and experimental data, revealing that PLA was not consumed while killing the bacteria. Another kinetic model was established to describe the production of PLA by the engineered Escherichia coli. This model was then used to calculate the minimum biomass of E. coli to produce the MBC of PLA. The proposed models help to understand the inhibitory pattern of PLA, serving as a theoretical guide for the selection an appropriate strain to improve the product shelf-life.
Acknowledgements
This work was partially supported by the National Natural Science Foundation of China (Funder Id: http://dx.doi.org/10.13039/501100001809, Grant No. 11671335,21807011), Natural Science Foundation of Fujian Province (Funder Id: http://dx.doi.org/10.13039/501100003392, Grant No. 2018J01657,2018J01673) and the Cultivation Project of Distinguished Young Scientists in Fujian Universities and Colleges.
Contributions: Yuxian Chen and Jianxiong Ye contributed equally.
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- Role of Reaction Time on the Electrical Conductivity, Thermal Stability and Photoluminescence Property of Polyanilne Nanofibers
- Experimental Study on Bubble Size Distribution in Gas-Liquid Reversed Jet Loop Reactor
- Methylene Blue Adsorption onto Neem Leave/Chitosan Aggregates: Isotherm, Kinetics and Thermodynamics Studies
- Antibacterial Activity of Phenyllactic acid Against Staphylococcus Epidermidis and Its Microbial Production: Modelling and Optimization-Based Analysis
- A New Robust Approach for Reactor Network Synthesis by Combination of Mathematical Method and NSGAII
- Experimental Evaluation of Biomass Medium-Temperature Gasification with Rice Straw as the Fuel in a Bubbling Fluidized Bed Gasifier
- Effect of Catalyst (de)activation on Reagent Diffusion in ZSM-5/alumina Extruded Pellet for the Methanol-to-hydrocarbons Conversion
- Kinetics Investigation of Carbon Dioxide Hydrate Formation Process in a New Impinging Stream Reactor
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