Exogenous hydrogen sulfide exhibits anti-cancer effects though p38 MAPK signaling pathway in C6 glioma cells
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Lanfu Zhao
Abstract
It has been documented that H2S, in some types of cancer, promotes tumor proliferation, whereas, in the other types, it inhibits the tumor cell growth. In the present study, we investigated the anti-cancer effects and relevant mechanisms of NaHS in C6 glioma cells. C6 cells were subjected to different concentrations of NaHS, then cell viability and morphological changes were examined by MTT assay and Hoechst staining. The protein expression of Caspase-3, Bcl-2, Bax, p38 MAPK (mitogen-activated protein kinase), and p53 was measured by Western blotting. This work demonstrated that NaHS could reduce cell number and induce apoptosis of C6 gliomas cells. The protein expression of Caspase-3 and Bax was up-regulated, while the protein expression of Bcl-2 was down-regulated. Additionally, p38 MAPK and p53 were activated in response to NaHS. Moreover, p38 MAPK inhibitor, SB203580, counteracted the inhibitory effect of NaHS on C6 glioma cells. These data suggest that NaHS can effectively reduce cell number of C6 cells by triggering the apoptosis via Caspase-dependent pathway. p38 MAPK and p53 play an important role in NaHS-induced apoptosis in C6 cells. These findings imply that administration of NaHS may represent a new strategy for the treatment of glioma.
Acknowledgments
The authors wish to thank Guodong GAO from Department of Neurosurgery, Tangdu Hospital, the Fourth Military Medical University, for the critical reading of the manuscript. Laboratory support from Jing Wang is also gratefully acknowledged. The authors would also like to thank Rong Kuang for her assistance with the molecular biology components of this work.
Conflicts of interest: None to declare.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Reviews
- Defects of corneocyte structural proteins and epidermal barrier in atopic dermatitis
- C-reactive protein and inflammation: conformational changes affect function
- Biogenesis of mitochondrial outer membrane proteins, problems and diseases
- Hepatitis C virus and autophagy
- Minireviews
- The glucocorticoid receptor in inflammatory processes: transrepression is not enough
- Red blood cells in Rett syndrome: oxidative stress, morphological changes and altered membrane organization
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- High β-glucosidase (GBA) activity not attributable to GBA1 and GBA2 in live normal and enzyme-deficient fibroblasts may emphasise the role of additional GBAs
- Cell Biology and Signaling
- Exogenous hydrogen sulfide exhibits anti-cancer effects though p38 MAPK signaling pathway in C6 glioma cells
- Novel Techniques
- Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells
- A simple and highly sensitive method of measuring heme oxygenase activity
Artikel in diesem Heft
- Frontmatter
- Reviews
- Defects of corneocyte structural proteins and epidermal barrier in atopic dermatitis
- C-reactive protein and inflammation: conformational changes affect function
- Biogenesis of mitochondrial outer membrane proteins, problems and diseases
- Hepatitis C virus and autophagy
- Minireviews
- The glucocorticoid receptor in inflammatory processes: transrepression is not enough
- Red blood cells in Rett syndrome: oxidative stress, morphological changes and altered membrane organization
- Research Articles/Short Communications
- Membranes, Lipids, Glycobiology
- High β-glucosidase (GBA) activity not attributable to GBA1 and GBA2 in live normal and enzyme-deficient fibroblasts may emphasise the role of additional GBAs
- Cell Biology and Signaling
- Exogenous hydrogen sulfide exhibits anti-cancer effects though p38 MAPK signaling pathway in C6 glioma cells
- Novel Techniques
- Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells
- A simple and highly sensitive method of measuring heme oxygenase activity
