Startseite Medizin Comparison of two LC-MS/MS methods for the quantification of 24,25-dihydroxyvitamin D3 in patients and external quality assurance samples
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Comparison of two LC-MS/MS methods for the quantification of 24,25-dihydroxyvitamin D3 in patients and external quality assurance samples

  • Sieglinde Zelzer ORCID logo , Caroline Le Goff , Stéphanie Peeters , Chiara Calaprice , Andreas Meinitzer , Dietmar Enko , Walter Goessler , Markus Herrmann EMAIL logo und Etienne Cavalier ORCID logo
Veröffentlicht/Copyright: 2. November 2021
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Clinical Chemistry and Laboratory Medicine (CCLM)
Aus der Zeitschrift Clinical Chemistry and Laboratory Medicine (CCLM) Band 60 Heft 1

Abstract

Objectives

In-house developed liquid-chromatography mass spectrometry (LC-MS/MS) methods are used more and more frequently for the simultaneous quantification of vitamin D metabolites. Among these, 24,25-dihydroxyvitamin D3 (24,25(OH)2D3) is of clinical interest. This study assessed the agreement of this metabolite in two validated in-house LC-MS/MS methods.

Methods

24,25(OH)2D3 was measured in 20 samples from the vitamin D external quality assurance (DEQAS) program and in a mixed cohort of hospital patients samples (n=195) with the LC-MS/MS method at the Medical University of Graz (LC-MS/MS 1) and at the University of Liège (LC-MS/MS 2).

Results

In DEQAS samples, 24,25(OH)2D3 results with LC-MS/MS 1 had a proportional bias of 1.0% and a negative systemic difference of −0.05%. LC-MS/MS 2 also showed a proportional bias of 1.0% and the negative systemic bias was −0.22%. Comparing the EQA samples with both methods, no systemic bias was found (0.0%) and the slope was 1%. The mean difference of 195 serum sample measurements between the two LC-MS/MS methods was minimal (−0.2%). Both LC-MS/MS methods showed a constant bias of 0.31 nmol/L and a positive proportional bias of 0.90%, respectively.

Conclusions

This study is the first to assess the comparability of 24,25(OH)2D3 concentrations in a mixed cohort of hospitalized patients with two fully validated in-house LC-MS/MS methods. Despite different sample preparation, chromatographic separation and ionization, both methods showed high precision measurements of 24,25(OH)2D3. Furthermore, we demonstrate the improvement of accuracy and precision measurements of 24,25(OH)2D3 in serum samples and in the DEQAS program.

Keywords: 24,25-dihydroxyvitamin D (24,25(OH)2D); liquid-chromatography mass spectrometry (LC-MS/MS); method comparison; patient samples; vitamin D external quality assurance scheme (DEQAS)
Corresponding author: Prof. Markus Herrmann, MD, FRCPA, Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Auenbruggerplatz 15 8036 Graz, Austria, Phone: 0043 316 385 13145, Fax: 0043 316 385 13430, E-mail:

Acknowledgments

This study is part of the dissertation of the first author Zelzer S.

  1. Research funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

  2. Author contribution: Conceptualization, SZ, MH and EC; Data curation, SZ and EC; Formal analysis, SZ, CL and EC; Methodology, SZ, AM, SP and CC; Project administration, AM, DE, MH and EC; Supervision, CLP, SP, WG and HM; Writing – original draft, SZ and MH; Writing – review and editing, CLG, SP, CC, AM, DE, WG and EC. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  3. Competing interests: The authors declared that there is no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The ethical approval of this study was provided by the Ethical Committee of the Johannes Kepler University Linz (Linz, Austria) (Ref: C-108-16) and was carried out in accordance to the guidelines laid down in the Declaration of Helsinki 1964, and amended at the 59th World Medical Assembly, Seoul, Korea, October 2008.

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Received: 2021-07-13
Revised: 2021-08-27
Accepted: 2021-09-29
Published Online: 2021-11-02
Published in Print: 2022-01-26

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/cclm-2021-0792
Schlagwörter für diesen Artikel
24,25-dihydroxyvitamin D (24,25(OH)2D); liquid-chromatography mass spectrometry (LC-MS/MS); method comparison; patient samples; vitamin D external quality assurance scheme (DEQAS)

Artikel in diesem Heft

  1. Frontmatter
  2. Editorials
  3. EU health policy is on the brink of a spectacular own-goal that will harm patients and hamper innovation
  4. Theranos revisited: the trial and lessons learned
  5. Reviews
  6. Review and evolution of guidelines for diagnosis of COVID-19 vaccine induced thrombotic thrombocytopenia (VITT)
  7. High-sensitivity cardiac troponins in pediatric population
  8. Opinion Papers
  9. Implementation of the new EU IVD regulation – urgent initiatives are needed to avert impending crisis
  10. Exploration of novel biomarkers for hypertensive disorders of pregnancy by comprehensive analysis of peptide fragments in blood: their potential and technologies supporting quantification
  11. General Clinical Chemistry and Laboratory Medicine
  12. Total lab automation: sample stability of clinical chemistry parameters in an automated storage and retrieval module
  13. Instability of corticotropin during long-term storage – myth or reality?
  14. External quality assessment of serum indices: Spanish SEQC-ML program
  15. Comparison of two LC-MS/MS methods for the quantification of 24,25-dihydroxyvitamin D3 in patients and external quality assurance samples
  16. Isotope dilution LC-MS/MS quantification of the cystic fibrosis transmembrane conductance regulator (CFTR) modulators ivacaftor, lumacaftor, tezacaftor, elexacaftor, and their major metabolites in human serum
  17. Hematology and Coagulation
  18. The development of autoverification system of lymphocyte subset assays on the flow cytometry platform
  19. Cancer Diagnostics
  20. Comparison of the QuikRead go® point-of-care faecal immunochemical test for haemoglobin with the FOB Gold Wide® laboratory analyser to diagnose colorectal cancer in symptomatic patients
  21. Evaluation of circulating Dickkopf-1 as a prognostic biomarker in ovarian cancer patients
  22. Cardiovascular Diseases
  23. Soluble CD40 ligand and outcome in patients with coronary artery disease undergoing percutaneous coronary intervention
  24. Diabetes
  25. Lot variation and inter-device differences contribute to poor analytical performance of the DCA Vantage™ HbA1c POCT instrument in a true clinical setting
  26. Infectious Diseases
  27. Lipase elevation in serum of COVID-19 patients: frequency, extent of increase and clinical value
  28. Acknowledgment
  29. Acknowledgment
  30. Letters to the Editors
  31. Presepsin value predicts the risk of developing severe/critical COVID-19 illness: results of a pooled analysis
  32. Measuring accuracy of the neutralizing activity of COVID-19 convalescent plasma
  33. Evaluation of reference intervals for classical and alternative pathway functional complement assays
  34. Reference values for plasma neurofilament light chain in healthy Chinese children
  35. Cerebrospinal fluid neurogranin in Alzheimer’s disease studies: are immunoassay results interchangeable?
  36. Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels
  37. A conspicuous reduced plasma creatinine: the first presenting sign of Waldenstrom macroglobulinemia
  38. Effect of non-linearity on rheumatoid factor assay in Beckman system IMMAGE800
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