Startseite Medizin Measurement of the axial and circumferential mechanical properties of rat skin tissue at different anatomical locations
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Measurement of the axial and circumferential mechanical properties of rat skin tissue at different anatomical locations

  • Alireza Karimi , Maedeh Haghighatnama , Mahdi Navidbakhsh EMAIL logo und Afsaneh Motevalli Haghi
Veröffentlicht/Copyright: 11. November 2014

Abstract

Skin tissue is not only responsible for thermoregulation but also for protecting the human body from mechanical, bacterial, and viral insults. The mechanical properties of skin tissue may vary according to the anatomical locations in the body. However, the linear elastic and nonlinear hyperelastic mechanical properties of the skin in different anatomical regions and at different loading directions (axial and circumferential) so far have not been determined. In this study, the mechanical properties during tension of the rat abdomen and back were calculated at different loading directions using linear elastic and nonlinear hyperelastic material models. The skin samples were subjected to a series of tensile tests. The elastic modulus and maximum stress of the skin tissues were measured before the incidence of failure. The nonlinear mechanical behavior of the skin tissues was also computationally investigated through a constitutive equation. Hyperelastic strain energy density function was calibrated using the experimental data. The results revealed the anisotropic mechanical behavior of the abdomen and the isotropic mechanical response of the back skin. The highest elastic modulus was observed in the abdomen skin under the axial direction (10 MPa), while the lowest one was seen in the back skin under axial loading (5 MPa). The Mooney-Rivlin material model closely addressed the nonlinear mechanical behavior of the skin at different loading directions, which can be implemented in the future biomechanical models of skin tissue. The results might have implications not only for understanding of the isotropic and anisotropic mechanical behavior of skin tissue at different anatomical locations but also for providing more information for a diversity of disciplines, including dermatology, cosmetics industry, clinical decision making, and clinical intervention.


Corresponding author: Mahdi Navidbakhsh, School of Mechanical Engineering, Iran University of Science and Technology, Tehran 16846, Iran, Phone: +98 21 77209027, Fax: +98 21 73021585, E-mail: ; and Tissue Engineering and Biological Systems Research Laboratory, School of Mechanical Engineering, Iran University of Science and Technology, Tehran 16846, Iran

Acknowledgments

The authors acknowledge the Iran University of Science for funding this project.

Conflicts of interest statement: The authors declare that they have no conflicts of interest.

References

[1] Bhatti MA. Advanced topics in finite element analysis of structures. New York: John Wiley & Sons, Inc. 2006.Suche in Google Scholar

[2] Boyer G, Pailler Mattei C, Molimard J, Pericoi M, Laquieze S, Zahouani H. Non contact method for in vivo assessment of skin mechanical properties for assessing effect of ageing. Med Eng Phys 2012; 34: 172–178.10.1016/j.medengphy.2011.07.007Suche in Google Scholar PubMed

[3] Courtney T, Sacks MS, Stankus J, Guan J, Wagner WR. Design and analysis of tissue engineering scaffolds that mimic soft tissue mechanical anisotropy. Biomaterials 2006; 27: 3631–3638.10.1016/j.biomaterials.2006.02.024Suche in Google Scholar PubMed

[4] Crichton ML, Donose BC, Chen X, Raphael AP, Huang H, Kendall MAF. The viscoelastic, hyperelastic and scale dependent behaviour of freshly excised individual skin layers. Biomaterials 2011; 32: 4670–4681.10.1016/j.biomaterials.2011.03.012Suche in Google Scholar PubMed

[5] Cua AB, Wilhelm KP, Maibach HI. Elastic properties of human skin: relation to age, sex, and anatomical region. Arch Dermatol Res 1990; 282: 283–288.10.1007/BF00375720Suche in Google Scholar PubMed

[6] Delalleau A, Josse G, Lagarde JM, Zahouani H, Bergheau JM. A nonlinear elastic behavior to identify the mechanical parameters of human skin in vivo. Skin Res Tech 2008; 14: 152–164.10.1111/j.1600-0846.2007.00269.xSuche in Google Scholar PubMed

[7] Del Prete Z, Antoniucci S, Hoffman AH, Grigg P. Viscoelastic properties of skin in Mov-13 and Tsk mice. J Biomech 2004; 37: 1491–1497.10.1016/j.jbiomech.2004.01.015Suche in Google Scholar PubMed

[8] Evans SL. On the implementation of a wrinkling, hyperelastic membrane model for skin and other materials. Comput Methods Biomech Biomed Engin 2009; 12: 319–332.10.1080/10255840802546762Suche in Google Scholar PubMed

[9] Faghihi S, Gheysour M, Karimi A, Salarian R. Fabrication and mechanical characterization of graphene oxide-reinforced poly (acrylic acid)/gelatin composite hydrogels. J Appl Phys 2014; 115: 083513–083520.10.1063/1.4864153Suche in Google Scholar

[10] Faghihi S, Karimi A, Jamadi M, Imani R, Salarian R. Graphene oxide/poly(acrylic acid)/gelatin nanocomposite hydrogel: experimental and numerical validation of hyperelastic model. Mater Sci Eng: C 2014; 38: 299–305.10.1016/j.msec.2014.02.015Suche in Google Scholar PubMed

[11] Flynn C, Taberner A, Nielsen P. Mechanical characterisation of in vivo human skin using a 3D force-sensitive micro-robot and finite element analysis. Biomech Model Mechanobiol 2011; 10: 27–38.10.1007/s10237-010-0216-8Suche in Google Scholar PubMed

[12] Flynn C, Taberner A, Nielsen P. Measurement of the force–displacement response of in vivo human skin under a rich set of deformations. Med Eng Phys 2011; 33: 610–619.10.1016/j.medengphy.2010.12.017Suche in Google Scholar

[13] Groves RB, Coulman SA, Birchall JC, Evans SL. An anisotropic, hyperelastic model for skin: experimental measurements, finite element modelling and identification of parameters for human and murine skin. J Mech Behav Biomed Mater 2013; 18: 167–180.10.1016/j.jmbbm.2012.10.021Suche in Google Scholar

[14] Hillebrand GG, Liang Z, Yan X, Yoshii T. New wrinkles on wrinkling: an 8-year longitudinal study on the progression of expression lines into persistent wrinkles. Br J Dermatol 2010; 162: 1233–1241.10.1111/j.1365-2133.2010.09709.xSuche in Google Scholar

[15] Jenkins G. Molecular mechanisms of skin ageing. Mech Ageing Develop 2002; 123: 801–810.10.1016/S0047-6374(01)00425-0Suche in Google Scholar

[16] Kang G, Wu X. Ratchetting of porcine skin under uniaxial cyclic loading. J Mech Behav Biomed Mater 2011; 4: 498–506.10.1016/j.jmbbm.2010.12.015Suche in Google Scholar PubMed

[17] Karimi A, Navidbakhsh M. Measurement of the nonlinear mechanical properties of PVA sponge under longitudinal and circumferential loading. J Appl Polym Sci 2013; 131: 40257–40264.Suche in Google Scholar

[18] Karimi A, Navidbakhsh M. An experimental study on the mechanical properties of rat brain tissue using different stress-strain definitions. J Mater Sci Mater Med 2014; 25: 1623–1630.10.1007/s10856-014-5198-0Suche in Google Scholar PubMed

[19] Karimi A, Navidbakhsh M. Mechanical properties of PVA material for tissue engineering applications. Mater Tech Adv Perform Mater 2014; 29: 90–100.10.1179/1753555713Y.0000000115Suche in Google Scholar

[20] Karimi A, Navidbakhsh M. Response to the Letter to the Editor: Measurement of the uniaxial mechanical properties of healthy and atherosclerotic human coronary arteries. Mater Sci Eng: C 2014; 42: 421.10.1016/j.msec.2014.05.053Suche in Google Scholar PubMed

[21] Karimi A, Navidbakhsh M. Mechanical properties of polyvinyl alcohol sponge under different strain rates. Int J Mater Res 2014; 105: 404–408.10.3139/146.111036Suche in Google Scholar

[22] Karimi A, Navidbakhsh M. Material properties in unconfined compression of gelatin hydrogel for skin tissue engineering applications. Biomed. Eng.-Biomed. Tech. 2014; 59:479–486.Suche in Google Scholar

[23] Karimi A, Navidbakhsh M. Measurement of the uniaxial mechanical properties of rat skin using different stress–strain definitions. Skin Res Tech 2014; in press. DOI: 10.1111/srt.12171.10.1111/srt.12171Suche in Google Scholar PubMed

[24] Karimi A, Faturechi R, Navidbakhsh M, Hashemi SA. A nonlinear hyperelastic behavior to identify the mechanical properties of rat skin under uniaxial loading. J Mech Med Biol 2014; 14: 1450075–1450089.10.1142/S0219519414500754Suche in Google Scholar

[25] Karimi A, Navidbakhsh M, Alizadeh M, Razaghi R. A comparative study on the elastic modulus of polyvinyl alcohol sponge using different stress-strain definitions. Biomed Tech (Berl) 2014; 59: 439–446.10.1515/bmt-2013-0110Suche in Google Scholar PubMed

[26] Karimi A, Navidbakhsh M, Alizadeh M, Shojaei A. A comparative study on the mechanical properties of the umbilical vein and umbilical artery under uniaxial loading. Artery Res 2014; 8: 51–56.10.1016/j.artres.2014.02.001Suche in Google Scholar

[27] Karimi A, Navidbakhsh M, Beigzadeh B. A visco-hyperelastic constitutive approach for modeling polyvinyl alcohol sponge. Tissue Cell 2014; 46: 97–102.10.1016/j.tice.2013.12.004Suche in Google Scholar PubMed

[28] Karimi A, Navidbakhsh M, Beigzadeh B, Faghihi S. Hyperelastic mechanical behavior of rat brain infected by Plasmodium berghei ANKA-Experimental testing and constitutive modeling. Int J Damage Mech 2014; 23: 854–871.10.1177/1056789513514072Suche in Google Scholar

[29] Karimi A, Navidbakhsh M, Faghihi S. Measurement of the mechanical failure of PVA sponge using biaxial puncture test. J Biomater Tissue Eng 2014; 4: 46–50.10.1166/jbt.2014.1134Suche in Google Scholar

[30] Karimi A, Navidbakhsh M, Faghihi S. A comparative study on plaque vulnerability using constitutive equations. Perfusion 2014; 29: 179–184.10.1177/0267659113502835Suche in Google Scholar PubMed

[31] Karimi A, Navidbakhsh M, Faghihi S, Shojaei A, Hassani K. A finite element investigation on plaque vulnerability in realistic healthy and atherosclerotic human coronary arteries. Proc Inst Mech Eng H 2013; 227: 148–161.10.1177/0954411912461239Suche in Google Scholar PubMed

[32] Karimi A, Navidbakhsh M, Haghi AM. An experimental study on the structural and mechanical properties of polyvinyl alcohol sponge using different stress–strain definitions. Adv Polym Tech 2014; in press. DOI: 10.1002/adv.21441.10.1002/adv.21441Suche in Google Scholar

[33] Karimi A, Navidbakhsh M, Haghpanahi M. Constitutive model for numerical analysis of polyvinyl alcohol sponge under different strain rates. J Thermoplastic Composite Mater 2014; in press. DOI: 10.1177/0892705713520176.10.1177/0892705713520176Suche in Google Scholar

[34] Karimi A, Navidbakhsh M, Haghi AM, Faghihi S. Measurement of the uniaxial mechanical properties of rat brains infected by Plasmodium berghei ANKA. Proc Inst Mech Eng H 2013; 227: 609–614.10.1177/0954411913476779Suche in Google Scholar PubMed

[35] Karimi A, Navidbakhsh M, Razaghi R. An experimental-finite element analysis on the kinetic energy absorption capacity of polyvinyl alcohol sponge. Mater Sci Eng: C 2014; 39: 253–258.10.1016/j.msec.2014.03.009Suche in Google Scholar PubMed

[36] Karimi A, Navidbakhsh M, Razaghi R. Dynamic simulation and finite element analysis of the human mandible injury protected by polyvinyl alcohol sponge. Mater Sci Eng: C 2014; 42: 608–614.10.1016/j.msec.2014.06.001Suche in Google Scholar PubMed

[37] Karimi A, Navidbakhsh M, Razaghi R. A finite element study of balloon expandable stent for plaque and arterial wall vulnerability assessment. J Appl Phys 2014; 116: 044701–044710.10.1063/1.4891019Suche in Google Scholar

[38] Karimi A, Navidbakhsh M, Razaghi R. Plaque and arterial vulnerability investigation in a three-layer atherosclerotic human coronary artery using computational fluid–structure interaction method. J Appl Phys 2014; 116: 064701–064709.10.1063/1.4893368Suche in Google Scholar

[39] Karimi A, Navidbakhsh M, Razaghi R. Dynamic finite element simulation of the human head damage mechanics protected by polyvinyl alcohol sponge. Int J Damage Mech 2014; in press. DOI: 10.1177/1056789514535945.10.1177/1056789514535945Suche in Google Scholar

[40] Karimi A, Navidbakhsh M, Razaghi R, Haghpanahi M. A computational fluid-structure interaction model for plaque vulnerability assessment in atherosclerotic human coronary arteries. J Appl Phys 2014; 115: 144702–144711.10.1063/1.4870945Suche in Google Scholar

[41] Karimi A, Navidbakhsh M, Rezaee T, Hassani K. Measurement of the circumferential mechanical properties of the umbilical vein: experimental and numerical analyses. Comput Methods Biomech Biomed Engin 2014; in press. DOI: 10.1080/10255842.2014.910513.10.1080/10255842.2014.910513Suche in Google Scholar PubMed

[42] Karimi A, Navidbakhsh M, Shojaei A, Faghihi S. Measurement of the uniaxial mechanical properties of healthy and atherosclerotic human coronary arteries. Mater Sci Eng: C 2013; 33: 2550–2554.10.1016/j.msec.2013.02.016Suche in Google Scholar PubMed

[43] Karimi A, Navidbakhsh M, Shojaei A, Hassani K, Faghihi S. Study of plaque vulnerability in coronary artery using Mooney–Rivlin model: a combination of finite element and experimental method. Biomed Eng: Appl Basis Commun 2014; 26: 145–152.10.4015/S1016237214500136Suche in Google Scholar

[44] Karimi A, Navidbakhsh M, Yamada H, Razaghi R. A nonlinear finite element simulation of balloon expandable stent for assessment of plaque vulnerability inside a stenotic artery. Med Biol Eng Comput 2014; 52: 589–599.10.1007/s11517-014-1163-9Suche in Google Scholar

[45] Karimi A, Navidbakhsh M, Yamada H, Rezaii T, Hassani K. A comparative study on the quasilinear viscoelastic mechanical properties of the umbilical artery and the umbilical vein. Perfusion 2014; in press. DOI: 10.1177/0267659114536761.10.1177/0267659114536761Suche in Google Scholar

[46] Karimi A, Navidbakhsh M, Yousefi H, Haghi AM, Adnani Sadati SJ. Experimental and numerical study on the mechanical behavior of rat brain tissue. Perfusion 2014; 29: 307–314.10.1177/0267659114522088Suche in Google Scholar

[47] Karwoski AC, Plaut RH. Experiments on peeling adhesive tapes from human forearms. Skin Res Technol 2004; 10: 271–277.10.1111/j.1600-0846.2004.00082.xSuche in Google Scholar

[48] Kuwazuru O, Saothong J, Yoshikawa N. Mechanical approach to aging and wrinkling of human facial skin based on the multistage buckling theory. Med Eng Phys 2008; 30: 516–522.10.1016/j.medengphy.2007.06.001Suche in Google Scholar

[49] Lapeer RJ, Gasson PD, Karri V. Simulating plastic surgery: from human skin tensile tests, through hyperelastic finite element models to real-time haptics. Prog Biophys Mol Biol 2010; 103: 208–216.10.1016/j.pbiomolbio.2010.09.013Suche in Google Scholar

[50] Liang X, Boppart SA. Biomechanical properties of in vivo human skin from dynamic optical coherence elastography. IEEE Trans Biomed Eng 2010; 57: 953–959.10.1109/TBME.2009.2033464Suche in Google Scholar

[51] Lim KH, Chew CM, Chen PCY, et al. New extensometer to measure in vivo uniaxial mechanical properties of human skin. J Biomech 2008; 41: 931–936.10.1016/j.jbiomech.2008.01.004Suche in Google Scholar

[52] Lim J, Hong J, Chen WW, Weerasooriya T. Mechanical response of pig skin under dynamic tensile loading. Int J Impact Eng 2011; 38: 130–135.10.1016/j.ijimpeng.2010.09.003Suche in Google Scholar

[53] Lott-Crumpler DA, Chaudhry HR. Optimal patterns for suturing wounds of complex shapes to foster healing. J Biomech 2001; 34: 51–58.10.1016/S0021-9290(00)00160-3Suche in Google Scholar

[54] Muñoz MJ, Bea JA, Rodríguez JF, et al. An experimental study of the mouse skin behaviour: damage and inelastic aspects. J Biomech 2008; 41: 93–99.10.1016/j.jbiomech.2007.07.013Suche in Google Scholar

[55] Özyazgan İ, Liman N, Dursun N, Güneş I. The effects of ovariectomy on the mechanical properties of skin in rats. Maturitas 2002; 43: 65–74.10.1016/S0378-5122(02)00181-0Suche in Google Scholar

[56] Sasson A, Patchornik S, Eliasy R, Robinson D, Haj-Ali R. Hyperelastic mechanical behavior of chitosan hydrogels for nucleus pulposus replacement – experimental testing and constitutive modeling. J Mech Behav Biomed Mater 2012; 8: 143–153.10.1016/j.jmbbm.2011.12.008Suche in Google Scholar

[57] Shergold OA, Fleck NA, Radford D. The uniaxial stress versus strain response of pig skin and silicone rubber at low and high strain rates. Int J Impact Eng 2006; 32: 1384–1402.10.1016/j.ijimpeng.2004.11.010Suche in Google Scholar

[58] Silver FH, Kato YP, Ohno M, Wasserman AJ. Analysis of mammalian connective tissue: relationship between hierarchical structures and mechanical properties. J Long Term Eff Med Implants 1992; 2: 165–198.Suche in Google Scholar

[59] Thali MJ, Kneubuehl BP, Dirnhofer R. A “skin–skull–brain model” for the biomechanical reconstruction of blunt forces to the human head. Forensic Sci Int 2002; 125: 195–200.10.1016/S0379-0738(01)00639-9Suche in Google Scholar

[60] Tilleman TR, Tilleman MM, Neumann MH. The elastic properties of cancerous skin: Poisson’s ratio and Young’s modulus. Isr Med Assoc J 2004; 6: 753–755.Suche in Google Scholar

[61] Yang CS, Yeh CH, Chen MY, Jiang CH, Su FC, Yeh ML. Mechanical evaluation of the influence of different suture methods on temporal skin healing. Dermatol Surg 2009; 35: 1880–1885.10.1111/j.1524-4725.2009.01309.xSuche in Google Scholar PubMed

[62] Zhang Y, Brodell RT, Mostow EN, Vinyard CJ, Marie H. In vivo skin elastography with high-definition optical videos. Skin Res Tech 2009; 15: 271–282.10.1111/j.1600-0846.2009.00351.xSuche in Google Scholar PubMed

Received: 2014-6-3
Accepted: 2014-10-14
Published Online: 2014-11-11
Published in Print: 2015-4-1

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