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Translation termination and protein folding pathway genes are not correlated in gastric cancer
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Joana Malta-Vacas
Veröffentlicht/Copyright:
2. März 2009
Abstract
Background: The eukaryotic release factor 3 (eRF3) has been shown to affect both tubulin and actin cytoskeleton, suggesting a role in cytoskeleton assembly, mitotic spindle formation and chromosome segregation. Also, direct interactions between eRF3 and subunits of the cytosolic chaperonin CCT have been described. Moreover, both eRF3a and CCT subunits have been described to be up-regulated in cancer tissues. Our aim was to evaluate the hypothesis that eRF3 expression levels are correlated with the expression of genes encoding proteins involved in the tubulin folding pathways.
Methods: Relative expression levels of eRF1, eRF3a/GSPT1, PFDN4, CCT2, CCT4, and TBCA genes in tumour samples relative to their adjacent normal tissues were investigated using real time-polymerase chain reaction in 20 gastric cancer patients.
Results: The expression levels of eRF3a/GSPT1 were not correlated with the expression levels of the other genes studied. However, significant correlations were detected between the other genes, both within intestinal and diffuse type tumours.
Conclusions: eRF3a/GSPT1 expression at the mRNA level is independent from both cell translation rates and from the expression of the genes involved in tubulin-folding pathways. The differences in the patterns of expression of the genes studied support the hypothesis of genetically independent pathways in the origin of intestinal and diffuse type gastric tumours.
Clin Chem Lab Med 2009;47:427–31.
Keywords: CCT subunits; eukaryotic release factor 3a/G-to-S phase transition 1 (eRF3a/GSPT1); gastric cancer; protein folding pathways; translation termination
Received: 2008-9-5
Accepted: 2009-1-5
Published Online: 2009-03-02
Published in Print: 2009-04-01
©2009 by Walter de Gruyter Berlin New York
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Schlagwörter für diesen Artikel
CCT subunits;
eukaryotic release factor 3a/G-to-S phase transition 1 (eRF3a/GSPT1);
gastric cancer;
protein folding pathways;
translation termination
Artikel in diesem Heft
- Minireview
- Risk loci for type 2 diabetes – Quo vadis?
- Genetics and Molecular Diagnostics
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- Genotype distribution of estrogen receptor α polymorphisms in pregnant women from healthy and preeclampsia populations and its relation to blood pressure levels
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