Antibody titer against malondialdehyde-modified LDL compares with HDL cholesterol concentration in identifying angiographically verified coronary artery disease. Comparison of tests by ROC analysis
-
Riikka Rontu
Abstract
Antibody titer against malondialdehyde (MDA)-modified low-density lipoprotein (LDL) has been found to be associated with atherosclerosis, but it has not been established whether it would detect subjects with coronary artery disease (CAD). In the present study, receiver-operating characteristic (ROC) analysis was used to compare the diagnostic accuracy of the antibody titer against MDA-modified LDL and high-density lipoprotein (HDL) and LDL cholesterol levels in discrimination between subjects with (n=51) and without (n=35) angiographically verified 3-vessel CAD. As a result, the antibody titer against MDA-modified LDL was lower in subjects with CAD compared with subjects without CAD (p<0.0001). The area under the ROC plot was 0.822 (95% CI, 0.727 to 0.918) for the antibody titer and 0.769 (95% CI, 0.661 to 0.876) for the HDL cholesterol concentration. Both the antibody titer and the plasma HDL cholesterol level were more accurate markers of CAD than the LDL cholesterol level. As a conclusion, our results indicate that the antibody titer against MDA-modified LDL discriminates between subjects with widespread CAD and those without CAD similarly as the HDL cholesterol concentration. Moreover, the antibody titer against MDA-modified LDL is inversely correlated with the risk of severe CAD.
References
1 Hammer A, Kager G, Dohr G, Rabl H, Ghassempur I, Jurgens G. Generation, characterization, and histochemical application of monoclonal antibodies selectively recognizing oxidatively modified apoB-containing serum lipoproteins. Arterioscler Thromb Vasc Biol 1995; 15: 704–13. 10.1161/01.ATV.15.5.704Suche in Google Scholar
2 Yla-Herttuala S. Is oxidized low-density lipoprotein present in vivo? Curr Opin Lipidol 1998; 9: 337–44. 10.1097/00041433-199808000-00009Suche in Google Scholar
3 Freigang S, Horkko S, Miller E, Witztum JL, Palinski W. Immunization of LDL receptor-deficient mice with homologous malondialdehyde-modified and native LDL reduces progression of atherosclerosis by mechanisms other than induction of high titers of antibodies to oxidative neoepitopes. Arterioscler Thromb Vasc Biol 1998; 18: 1972–82. 10.1161/01.ATV.18.12.1972Suche in Google Scholar
4 Zhou X, Caligiuri G, Hamsten A, Lefvert AK, Hansson GK. LDL immunization induces T-cell-dependent antibody formation and protection against atherosclerosis. Arterioscler Thromb Vasc Biol 2001; 21: 108–14. 10.1161/01.ATV.21.1.108Suche in Google Scholar
5 Binder CJ, Hartvigsen K, Chang MK, Miller M, Broide D, Palinski W, et al. IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis. J Clin Invest 2004; 114: 427–37. 10.1172/JCI200420479Suche in Google Scholar
6 Karvonen J, Paivansalo M, Kesaniemi YA, Horkko S. Immunoglobulin M type of autoantibodies to oxidized low-density lipoprotein has an inverse relation to carotid artery atherosclerosis. Circulation 2003; 108: 2107–12. 10.1161/01.CIR.0000092891.55157.A7Suche in Google Scholar
7 Hulthe J, Bokemark L, Fagerberg B. Antibodies to oxidized LDL in relation to intima-media thickness in carotid and femoral arteries in 58-year-old subjectively clinically healthy men. Arterioscler Thromb Vasc Biol 2001; 21: 101–7. 10.1161/01.ATV.21.1.101Suche in Google Scholar
8 Wiklund O, Witztum JL, Carew TE, Pittman RC, Elam RL, Steinberg D. Turnover and tissue sites of degradation of glucosylated low density lipoprotein in normal and immunized rabbits. J Lipid Res 1987; 28: 1098–109. 10.1016/S0022-2275(20)38623-5Suche in Google Scholar
9 Judkins MP. Selective coronary arteriography. I. A percutaneous transfemoral technique. Radiology 1967; 89: 815–24. 10.1148/89.5.815Suche in Google Scholar PubMed
10 Lehtimaki T, Lehtinen S, Solakivi T, Nikkila M, Jaakkola O, Jokela H, et al. Autoantibodies against oxidized low density lipoprotein in patients with angiographically verified coronary artery disease. Arterioscler Thromb Vasc Biol 1999; 19: 23–7. 10.1161/01.ATV.19.1.23Suche in Google Scholar
11 Uusitupa MI, Niskanen L, Luoma J, Vilja P, Mercuri M, Rauramaa R, Yla-Herttuala S. Autoantibodies against oxidized LDL do not predict atherosclerotic vascular disease in non-insulin-dependent diabetes mellitus. Arterioscler Thromb Vasc Biol 1996; 16: 1236–42. 10.1161/01.ATV.16.10.1236Suche in Google Scholar
12 Salonen JT, Yla-Herttuala S, Yamamoto R, Butler S, Korpela H, Salonen R, et al. Autoantibody against oxidised LDL and progression of carotid atherosclerosis. Lancet 1992; 339: 883–7. 10.1016/0140-6736(92)90926-TSuche in Google Scholar
13 Tornvall P, Waeg G, Nilsson J, Hamsten A, Regnstrom J. Autoantibodies against modified low-density lipoproteins in coronary artery disease. Atherosclerosis 2003; 167: 347–53. 10.1016/S0021-9150(03)00021-2Suche in Google Scholar
14 Cherubini A, Mecocci P, Senin U, Lowenthal DT, Barnert G, Chionne F. Autoantibodies against oxidized low-density lipoproteins in older stroke patients. J Am Geriatr Soc 1997; 45: 125. 10.1111/j.1532-5415.1997.tb01001.xSuche in Google Scholar
15 Schumacher M, Eber B, Tatzber F, Kaufmann P, Halwachs G, Fruhwald FM, et al. Transient reduction of autoantibodies against oxidized LDL in patients with acute myocardial infarction. Free Radic Biol Med 1995; 18: 1087–91. 10.1016/0891-5849(94)00216-7Suche in Google Scholar
16 Griffith RL, Virella GT, Stevenson HC, Lopes-Virella MF. Low density lipoprotein metabolism by human macrophages activated with low density lipoprotein immune complexes. A possible mechanism of foam cell formation. J Exp Med 1988; 168: 1041–59. 10.1084/jem.168.3.1041Suche in Google Scholar PubMed PubMed Central
© by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent
- Antibody titer against malondialdehyde-modified LDL compares with HDL cholesterol concentration in identifying angiographically verified coronary artery disease. Comparison of tests by ROC analysis
- Fucosylation of serum glycoproteins in lung cancer patients
- Association of cytochrome P450 2E1 genetic polymorphisms with squamous cell carcinoma of the oesophagus
- α-Tocopherol as an antiretroviral therapy supplement for HIV-1-infected patients for increased lymphocyte viability
- The effect of antioxidant supplementation on superoxide dismutase activity, Cu and Zn levels, and total antioxidant status in erythrocytes of patients with Graves' disease
- Concentrations of calcium, copper, iron, magnesium, potassium, sodium and zinc in adult female hair with different body mass indexes in Taiwan
- Estimation of the limit of detection with a bootstrap-derived standard error by a partly non-parametric approach. Application to HPLC drug assays
- Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry
- How accurate are clinical activity indices for scoring of disease activity in inflammatory bowel disease (IBD)?
- An iterative method for improved estimation of the mean of peer-group distributions in proficiency testing
- Impact of a common CV evaluation scheme on overall laboratory performance: 8-year experience of a large national proficiency testing program in Japan
- Significance of small dense low-density lipoproteins as coronary risk factor in diabetic and non-diabetic Korean populations
- Evaluation of a rapid bedside test for the quantitative determination of C-reactive protein
- Power function of the reference change value in relation to cut-off points, reference intervals and index of individuality
- Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay
- Vitamin C and aberrant electrolyte results
- POX-ACT assay and d-ROMs test: comparison impossible
- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
Artikel in diesem Heft
- Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent
- Antibody titer against malondialdehyde-modified LDL compares with HDL cholesterol concentration in identifying angiographically verified coronary artery disease. Comparison of tests by ROC analysis
- Fucosylation of serum glycoproteins in lung cancer patients
- Association of cytochrome P450 2E1 genetic polymorphisms with squamous cell carcinoma of the oesophagus
- α-Tocopherol as an antiretroviral therapy supplement for HIV-1-infected patients for increased lymphocyte viability
- The effect of antioxidant supplementation on superoxide dismutase activity, Cu and Zn levels, and total antioxidant status in erythrocytes of patients with Graves' disease
- Concentrations of calcium, copper, iron, magnesium, potassium, sodium and zinc in adult female hair with different body mass indexes in Taiwan
- Estimation of the limit of detection with a bootstrap-derived standard error by a partly non-parametric approach. Application to HPLC drug assays
- Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry
- How accurate are clinical activity indices for scoring of disease activity in inflammatory bowel disease (IBD)?
- An iterative method for improved estimation of the mean of peer-group distributions in proficiency testing
- Impact of a common CV evaluation scheme on overall laboratory performance: 8-year experience of a large national proficiency testing program in Japan
- Significance of small dense low-density lipoproteins as coronary risk factor in diabetic and non-diabetic Korean populations
- Evaluation of a rapid bedside test for the quantitative determination of C-reactive protein
- Power function of the reference change value in relation to cut-off points, reference intervals and index of individuality
- Erythrocyte ferritin concentration: analytical performance of the immunoenzymatic IMx-Ferritin (Abbott) assay
- Vitamin C and aberrant electrolyte results
- POX-ACT assay and d-ROMs test: comparison impossible
- A spectrophotometric micromethod for determining erythrocyte protoporphyrin-IX in whole blood or erythrocytes
