Cellular distribution of human tissue kallikreins: immunohistochemical localization
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Abstract
We have studied the immunohistochemical expression (IE) of eight non-tissue-specific human kallikreins (hKs) (hK5, 6, 7, 10, 11, 12, 13, and 14) in different normal tissues. The IE was always cytoplasmic, showing a characteristic pattern in some tissues. Comparison of the IE of all hKs studied in the different tissues revealed no major differences, suggesting that they share a common mode of regulation. Furthermore, hKs were immunohistochemically revealed in a variety of tissues, indicating that no protein is tissue-specific (except for hK2 and hK3, which have tissue-restricted expression). In general, our results correspond well with data from RT-PCR and ELISA assays. Glandular epithelia constitute the main kallikrein IE sites, and the staining in their secretions confirms that these proteases are secreted. A variety of other tissues express the proteins as well. We have also immunohistochemically evaluated all the above hKs in several malignant tissues. Tumors arising from tissues expressing kallikreins tested positive. Corresponding to the IE in normal glandular tissues, most hKs were expressed in adenocarcinomas. The prognostic value of several hKs was studied in series of prostate, renal cell, colon and urothelial carcinomas.
References
Abrahamsson, P.A., Lilja, H., Falkmer, S., and Wadstrom, L.B. (1988). Immunohistochemical distribution of the three predominant secretory proteins in the parenchyma of hyperplastic and neoplastic prostate glands. Prostate12, 39–46.10.1002/pros.2990120106Suche in Google Scholar
Bhoola, K.D., Figueroa, C.D., and Worthy, K. (1992). Bioregulation of kinins: kallikreins, kininogens, and kininases. Pharmacol. Rev.44, 1–80.Suche in Google Scholar
Bhoola, K., Ramsaroop, R., Plendl, J., Cassim, B., Dlamini, Z., and Naicker, S. (2001). Kallikrein and kinin receptor expression in inflammation and cancer. Biol. Chem.382, 77–89.10.1515/BC.2001.013Suche in Google Scholar
Black, M.H., and Diamandis, E.P. (2000). The diagnostic and prognostic utility of prostate specific antigen for diseases of the breast. Breast Cancer Res. Treat.59, 1–4.10.1023/A:1006380306781Suche in Google Scholar
Black, M.H., Magklara, A., Obiezu, C., Levesque, M.A., Sutherland, D.J., Tindall, D.J., Young, C.Y., Sauter, E.R., and Diamandis, E.P. (2000). Expression of a prostate-associated protein, human glandular kallikrein (hK2), in breast tumors and in normal breast secretions. Br. J. Cancer82, 361–367.10.1054/bjoc.1999.0927Suche in Google Scholar
Borgono, C.A. and Diamandis, E.P. (2004). The emerging roles of human tissue kallikreins in cancer. Nat. Rev. Cancer4, 876–890.10.1038/nrc1474Suche in Google Scholar
Cane, S., Bignotti, E., Bignotti, E., Bellone, S., Palmieri, M., De las Casas, L., Roman, J.J., Pecorelli, S., Cannon, M.J., O'Brien, T., and Santin, A.D. (2004). The novel serine protease tumor-associated differentially expressed gene-14 (KLK8/neuropsin/ovasin) is highly overexpressed in cervical cancer. Am. J. Obstet. Gynecol.190, 60–66.10.1016/j.ajog.2003.07.020Suche in Google Scholar
Chang, A., Yousef, G.M., Jung, K., Rajpert-de Meyts, E., and Diamandis, E.P. (2001). Identification and molecular characterization of five novel kallikrein gene (KLK13; KLK14) splice variants: differential expression in the human testis and testicular cancer. Anticancer Res.21, 3147–3152.Suche in Google Scholar
Chang, A., Yousef, G.M., Scorilas, A., Grass, L., Sismondi, P., Ponzone, R., and Diamandis, E.P. (2002). Human kallikrein gene 13 (KLK13) expression by quantitative RT-PCR: an independent indicator of favourable prognosis in breast cancer. Br. J. Cancer86, 1457–1464.10.1038/sj.bjc.6600283Suche in Google Scholar
Chao, J., Sostek, M., Shimamoto, K., Bank, H.L., Bigelow, J., and Margolius, H.S. (1980). Kallikrein content of rat pancreatic acinar cells or islets by direct radioimmunoassay. Hoppe-Seyler's Z. Physiol. Chem.361, 1805–1810.10.1515/bchm2.1980.361.2.1805Suche in Google Scholar
Chu, T.M. (1997). Prostate-specific antigen and early detection of prostate cancer. Tumor Biol.18, 123–134.10.1159/000218023Suche in Google Scholar
Clements, J.A. (1998). Current perspectives on the molecular biology of the renal tissue kallikrein gene and the related tissue kallikrein gene family. Biol. Res.31, 151–159.Suche in Google Scholar
Clements, J. and Mukhtar, A. (1994). Glandular kallikreins and prostate-specific antigen are expressed in the human endometrium. J. Clin. Endocrinol. Metab.78, 1536–1539.Suche in Google Scholar
Clements, J. and Mukhtar, A. (1997). Tissue kallikrein and the bradyinin B2 receptor are expressed in endometrial and prostate cancers. Immunopharmacology36, 217–220.10.1016/S0162-3109(97)00024-6Suche in Google Scholar
Clements, J., Mukhtar, A., Ehrlich, A., and Yap, B. (1994). Glandular kallikrein gene expression in the human uterus. Braz. J. Med. Biol. Res.27, 1853–1863.Suche in Google Scholar
Clements, J.A., Mukhtar, A., Verity, K., Pullar, M., McNeill, P., Cummins, J., and Fuller, P.J. (1996). Kallikrein gene expression in human pituitary tissues. Clin. Endocrinol.44, 223–231.10.1046/j.1365-2265.1996.661481.xSuche in Google Scholar
Clements, J.A., Willemsen, N.M., Myers, S.A., and Dong, Y. (2004). The tissue kallikrein family of serine proteases: functional roles in human disease and potential as clinical biomarkers. Crit. Rev. Clin. Lab. Sci.41, 265–312.10.1080/10408360490471931Suche in Google Scholar
Cumming, A.D., Walsh, T., Wojtacha, D., Fleming, S., Thomson, D., and Jenkins, D.A. (1994). Expression of tissue kallikrein in human kidney. Clin. Sci. (Lond.)87, 5–11.10.1042/cs0870005Suche in Google Scholar
Darson, M.F., Pacelli, A., Roche, P., Rittenhouse, H.G., Wolfert, R.L., Young, C.Y., Klee, G.G., Tindall, D.J., and Bostwick, D.G. (1997). Human glandular kallikrein 2 (hK2) expression in prostate intraepithelial neoplasia and adenocarcinoma: a novel prostate cancer marker. Urology49, 857–862.10.1016/S0090-4295(97)00108-8Suche in Google Scholar
Darson, M.F., Pacelli, A., Roche, P., Rittenhouse, H.G., Wolfert, R.L., Saeid, M.S., Young, C.Y., Klee, G.G., Tindall, D.J., and Bostwick, D.G. (1999). Human glandular kallikrein 2 expression in prostate adenocarcinoma and lymph node metastases. Urology53, 939–944.10.1016/S0090-4295(98)00637-2Suche in Google Scholar
Davidson, B., Xi, Z., Klokk, T.I., Trope, C.G., Dorum, A., Scheistroen, M., and Saatcioglu, F. (2005). Kallikrein 4 expression is up-regulated in epithelial ovarian carcinoma cells in effusions. Am. J. Clin. Pathol.123, 360–368.10.1309/PTBB5BPCKX8K9V69Suche in Google Scholar
Dedio, J., Wiemer, G., Rutten, H., Dendorfer, A., Scholkens, B.A., Müller-Esterl, W., and Wohlfart, P. (2001). Tissue kallikrein KLK1 is expressed de novo in endothelial cells and mediates relaxation of human umbilical veins. Biol. Chem.382, 1483–1490.10.1515/BC.2001.182Suche in Google Scholar
Diamandis, E.P., and Yousef, G.M. (2001). Human tissue kallikrein gene family: a rich source of novel disease biomarkers. Expert Rev. Mol. Diagn.1, 182–190.10.1586/14737159.1.2.182Suche in Google Scholar
Diamandis, E.P., Yousef, G.M., Clements, J., Ashworth, L.K., Yoshida, S., Egelrud, T., Nelson, P.S., Shiosaka, S., Little, S., Lilja, H., et al. (2000a). New nomenclature for the human tissue kallikrein gene family. Clin. Chem.46, 1855–1858.10.1093/clinchem/46.11.1855Suche in Google Scholar
Diamandis, E.P., Yousef, G.M., Luo, L.Y., Magklara, A., and Obiezu, C.V. (2000b). The new human kallikrein gene family: implications in carcinogenesis. Trends Endocrinol. Metab.11, 54–60.10.1016/S1043-2760(99)00225-8Suche in Google Scholar
Diamandis, E.P., Yousef, G.M., Petraki, C., and Soosaipillai, A.R. (2000c). Human kallikrein 6 as a biomarker of Alzheimer's disease. Clin. Biochem.33, 663–667.10.1016/S0009-9120(00)00185-5Suche in Google Scholar
Diamandis, E.P., Yousef, G.M., Soosaipillai, A.R., Grass, L., Porter, A., Little, S., and Sotiropoulou, G. (2000d). Immunofluorometric assay of human kallikrein 6 (zyme/protease M/neurosin) and preliminary clinical applications. Clin. Biochem.33, 369–375.10.1016/S0009-9120(00)00145-4Suche in Google Scholar
Diamandis, E.P., Okui, A., Mitsui, S., Luo, L.Y., Soosaipillai, A., Grass, L., Nakamura, T., Howarth, D.J., and Yamaguchi, N. (2002). Human kallikrein 11: a new biomarker of prostate and ovarian carcinoma. Cancer Res.62, 295–300.Suche in Google Scholar
Dietl, T., Kruck, J., and Fritz, H. (1978). Localization of kallikrein in porcine pancreas and submandibular gland as revealed by the indirect immunofluorescence technique. Hoppe-Seyler's Z. Physiol. Chem.359, 499–505.10.1515/bchm.1978.359.1.499Suche in Google Scholar PubMed
Dong, Y., Kaushal, A., Bui, L., Chu, S., Fuller, P.J., Nicklin, J., Samaratunga, H., and Clements, J. (2001). Human kallikrein 4 (KLK4) is highly expressed in serous ovarian carcinomas. Clin. Cancer Res.7, 2363–2371.Suche in Google Scholar
Dong, Y., Kaushal, A., Brattsand, M., Nicklin, J., and Clements, J.A. (2003). Differential splicing of KLK5 and KLK7 in epithelial ovarian cancer produces novel variants with potential as cancer biomarkers. Clin. Cancer Res.9, 1710–1720.Suche in Google Scholar
Dong, Y., Bui, L.T., Odorico, D.M., Tan, O.L., Meyers, S.A., Samaratunga, H., Gardiner, R.A., and Clements, J.A. (2005). Compartmentalized expression of kallikrein 4 (KLK4/hK4) isoforms in prostate cancer: nuclear, cytoplasmic and secreted forms. Endocr. Relat. Cancer12, 875–889.10.1677/erc.1.01062Suche in Google Scholar PubMed
Gallee, M.P., Visser-de Jong, E., van der Korput, J.A., van der Kwast, T.H., ten Kate, F.J., Schroeder, F.H., and Trapman, J. (1990). Variation of prostate-specific antigen expression in different tumour growth patterns present in prostatectomy specimens. Urol. Res.18, 181–187.10.1007/BF00295844Suche in Google Scholar PubMed
Hoffman, B.R., Katsaros, D., Scorilas, A., Diamandis, P., Fracchioli, S., Rigault de la Longrais, I.A., Colgan, T., Puopolo, M., Giardina, G., Massobrio, M., and Diamandis, E.P. (2002). Immunofluorometric quantitation and histochemical localization of kallikrein 6 protein in ovarian cancer tissue: a new independent unfavourable prognostic biomarker. Br. J. Cancer87, 763–771.10.1038/sj.bjc.6600533Suche in Google Scholar PubMed PubMed Central
Jaffa, A.A., Chai, K.X., Chao, J., Chao, L., and Mayfield, R.K. (1992). Effects of diabetes and insulin on expression of kallikrein and rennin genes in the kidney. Kidney Int.41, 789–795.10.1038/ki.1992.122Suche in Google Scholar PubMed
Jones, T.H., Figueroa, C.D., Smith, C., Cullen, D.R., and Bhoola, K.D. (1990). Characterization of a tissue kallikrein in human prolactin-secreting adenomas. J. Endocrinol.124, 327–331.10.1677/joe.0.1240327Suche in Google Scholar PubMed
Junes, M.J., Neuschatz, A.C., Bornstein, L.E., Naber, S.P., Band, V., and Wazer, D.E. (2003). Loss of expression of the putative tumor suppressor NES1 gene in biopsy-proven ductal carcinoma in situ predicts for invasive carcinoma at definitive surgery. Int. J. Radiat. Oncol. Biol. Phys.56, 653–657.Suche in Google Scholar
Kim, H., Scorilas, A., Katsaros, D., Yousef, G.M., Massobrio, M., Fracchioli, S., Piccinno, R., Gordini, G., and Diamandis, E.P. (2001). Human kallikrein gene 5 (KLK5) expression is an indicator of poor prognosis in ovarian cancer. Br. J. Cancer84, 643–650.10.1054/bjoc.2000.1649Suche in Google Scholar PubMed PubMed Central
Kishi, T., Grass, L., Soosaipillai, A., Shimizu-Okabe, and Diamandis, E.P. (2003). Human kallikrein 8: immunoassay development and identification in tissue extracts and biological fluids. Clin. Chem.49, 87–96.10.1373/49.1.87Suche in Google Scholar PubMed
Komatsu, N., Takata, M., Otsuki, N., Toyama, T., Ohka, R., Takehara, K., and Saijoh, K. (2003). Expression and localization of tissue kallikrein mRNAs in human epidermis and appendages. J. Invest. Dermatol.121, 542–549.10.1046/j.1523-1747.2003.12363.xSuche in Google Scholar
Komatsu, N., Saijoh, K., Toyama, T., OhKa, R., Otsuki, N., Hussack, G., Takehara, K., and Diamandis, E.P. (2005). Multiple tissue kallikrein mRNA and protein expression in normal skin and skin diseases. Br. J. Dermatol.153, 274–281.10.1111/j.1365-2133.2005.06754.xSuche in Google Scholar
Laxmikanthan, G., Blaber, S.I., Bernett, M.J., Scarisbrick, I.A., Aparecida Juliano, M., and Blaber, M. (2005). 1.70 Å X-ray structure of human apo kallikrein 1: structural changes upon peptide inhibitor/substrate binding. Proteins58, 802–814.Suche in Google Scholar
Little, S.P., Dixon, E.P., Norris, F., Buckley, W., Becker, G.W., Johnson, M., Dobbins, J.R., Wyrick, T., Miller, J.R., MacKellar, W., et al. (1997). Zyme, a novel and potentially amyloidogenic enzyme cDNA isolated from Alzheimer's disease brain. J. Biol. Chem.272, 25135–25142.10.1074/jbc.272.40.25135Suche in Google Scholar
Luo, L.Y., Katsaros, D., Scorilas, A., Fracchioli, S., Piccinno, R., Rigault de la Longrais, I.A., Howarth, D.J.C., and Diamandis, E.P. (2001). Prognostic value of human kallikrein 10 expression in epithelial ovarian carcinoma. Clin. Cancer Res.7, 2372–2379.Suche in Google Scholar
Luo, L.Y., Diamandis, E.P., Look, M.P., Soosaipillai, A.P., and Foekens, J.A. (2002). Higher expression of human kallikrein 10 in breast cancer tissue predicts tamoxifen resistance. Br. J. Cancer86, 1790–1796.10.1038/sj.bjc.6600323Suche in Google Scholar
Luo, L.Y., Yousef, G., and Diamandis, E.P. (2003). Human tissue kallikreins and testicular cancer. ARMIS111, 225–233.Suche in Google Scholar
Magklara, A., Cheung, C.C., Asa, S.L., and Diamandis, E.P. (2000). Expression of prostate-specific antigen and human glandular kallikrein 2 in the thyroid gland. Clin. Chim. Acta300, 171–180.10.1016/S0009-8981(00)00319-3Suche in Google Scholar
Margolius, H.S., Horwitz, D., Pisano, J.J., and Keiser, H.R. (1974). Urinary kallikrein excretion in hypertensive man. Relationships to sodium intake and sodium-retaining steroids. Circ. Res.35, 820–825.10.1161/01.RES.35.6.820Suche in Google Scholar
Margolius, H.S. (1998). Kallikreins, kinins and cardiovascular diseases: a short review. Biol. Res.31, 135–141.Suche in Google Scholar
McCormack, R.T., Rittenhouse, H.G., Finlay, J.A., Sokoloff, R.L., Wang, T.J., Wolfert, R.L., Lilja, H., and Oesterling, J. (1995). Molecular forms of prostate-specific antigen and the human kallikrein gene family: a new era. Urology45, 729–744.10.1016/S0090-4295(99)80076-4Suche in Google Scholar
Mitsui, S., Yamada, T., Okui, A., Kominami, K., Uemura, H., and Yamaguchi, N. (2000). A novel isoform of a kallikrein-like protease, TLSP/hippostatin (PRSS20), is expressed in the human brain and prostate. Biochem. Biophys. Res. Commun.272, 205–211.10.1006/bbrc.2000.2761Suche in Google Scholar
Mitsui, S., Okui, A., Uemura, H., Mizuno, T., Yamada, T., Yamamura, Y., and Yamaguchi, N. (2002). Decreased cerebrospinal fluid levels of neurosin (KLK6), an aging-related protease, as a possible new risk factor for Alzheimer's disease. Ann. NY Acad. Sci.977, 216–223.10.1111/j.1749-6632.2002.tb04818.xSuche in Google Scholar
Naicker, T., Khedun, S.M., Moodley, D., and Bhoola, K. (1997). Localisation of tissue kallikrein in the kidney of black African women with early onset pre-eclampsia: a pilot study. Immunopharmacology36, 249–254.10.1016/S0162-3109(97)00028-3Suche in Google Scholar
Naicker, S., Naidoo, S., Ramsaroop, R., Moodley, D., and Bhoola, K. (1999). Tissue kallikrein and kinins in renal disease. Immunopharmacology44, 183–192.10.1016/S0162-3109(99)00089-2Suche in Google Scholar
Obiezu, C.V., Katsaros, D., Massobrio, M., Scorilas, A., Yousef, G.M., Fracchioli, S., Rigault de la Longrais, I.A., Arisio, R., and Diamandis, E.P. (2001). Higher human kallikrein gene 4 (KLK4) expression indicates poor prognosis of ovarian cancer patients. Clin. Cancer Res.7, 2380–2386.Suche in Google Scholar
Obiezu, C.V., Soosaipillai, A., Jung, K., Stephan, C., Scorilas, A., Howarth, D.H., and Diamandis, E.P. (2002). Detection of human kallikrein 4 in healthy and cancerous prostatic tissues by immunofluorometry and immunohistochemistry. Clin. Chem.48, 1232–1240.10.1093/clinchem/48.8.1232Suche in Google Scholar
Obiezu, C.V., Shan, S.J., Soosaipillai, A., Luo, L.Y., Grass, L., Sotiropoulou, G., Petraki, C.D., Papanastasiou, P.A., Levesque, M.A., and Diamandis, E.P. (2005). Human kallikrein 4: quantitative study in tissues and evidence for its secretion into biological fluids. Clin. Chem.51, 1432–1442.10.1373/clinchem.2005.049692Suche in Google Scholar PubMed
ole-MoiYoi, O.K., Pinkus, G.S., Spragg, J., and Austen, K.F. (1979). Identification of human glandular kallikrein in the beta cell of the pancreas. N. Engl. J. Med.300, 1289–1294.10.1056/NEJM197906073002301Suche in Google Scholar PubMed
Orstavik, T.B., Brandtzaeg, P., Nustad, K., and Pierce, J.V. (1980). Immunohistochemical localization of kallikrein in human pancreas and salivary glands. J. Histochem. Cytochem.28, 557–562.10.1177/28.6.6156199Suche in Google Scholar PubMed
Orstavik, T.B., Brekke, I.B., Alumets, J., and Carretero, O.A. (1981). Kallikrein in rat pancreatic tissue after beta cell destruction or acinar cell atrophy. J. Histochem. Cytochem.29, 1431–1436.10.1177/29.12.6798105Suche in Google Scholar PubMed
Petraki, C.D., Karavana, V.N., Skoufogiannis, P.T., Little, S.P., Howarth, D.J.C., Yousef, G.M., and Diamandis, E.P. (2001). The spectrum of human kallikrein 6 (zyme/protease M/neurosin) expression in human tissues, as assessed by immunohistochemistry. J. Histochem. Cytochem.49, 1431–1442.10.1177/002215540104901111Suche in Google Scholar PubMed
Petraki, C.D., Karavana, V.N., Luo, L.Y., and Diamandis, E.P. (2002a). Human kallikrein 10 expression in normal tissues by immunohistochemistry. J. Histochem. Cytochem.50, 1247–1261.10.1177/002215540205000912Suche in Google Scholar PubMed
Petraki, C.D., Karavana, V.N., Revelos. K.I., Luo. L.Y., and Diamandis, E.P. (2002b). Immunohistochemical localization of human kallikreins 6 and 10 in pancreatic islets. Histochem. J.34, 313–322.Suche in Google Scholar
Petraki, C.D., Karavana, V.N., and Diamandis, E.P. (2003a). Human kallikrein 13 expression in normal tissues. An immunohistochemical study. J. Histochem. Cytochem.51, 493–501.10.1177/002215540305100411Suche in Google Scholar PubMed
Petraki, C.D., Papanastassiou, P.A., Gregorakis, A.K., Karavana, V.N., Luo, L.Y., and Diamandis, E.P. (2003b). Immunohistochemical localization of human kallikreins 6, 10, and 13 in benign and malignant prostatic tissues. Prostate Cancer Prostatic Dis.6, 223–227.10.1038/sj.pcan.4500674Suche in Google Scholar PubMed
Petraki, C.D., Gregorakis, A.K., Vaslamatzis, M.M., Papanastassiou, P., Yousef, G.M., and Diamandis, E.P. (2005). The immunohistochemical expression of human kallikreins 5, 6, 10 and 11 in renal cell carcinoma. Correlation with several prognostic factors. Tumor Biol.27, 1–7.Suche in Google Scholar
Pinkus, G.S., Maier, M., Seldin, D.C., ole-MoiYoi, O.K., Austen, K.F., and Spragg, J. (1983). Immunohistochemical localization of glandular kallikrein in the endocrine and exocrine human pancreas. J. Histochem. Cytochem.31, 1279–1288.10.1177/31.11.6194204Suche in Google Scholar
Pretlow, T.G., Pretlow, T.P., Yang, B., Kaetzel, C.S., Delmord, C.M., Kamis, S.M., Bodner, D.R., Kursh, E., Resnick, M.I., and Bradley, E.L. (1991). Tissue concentrations of prostate-specific antigen in prostatic carcinoma and benign prostatic hyperplasia. Int. J. Cancer49, 645–649.10.1002/ijc.2910490503Suche in Google Scholar
Rae, F., Bulmer, B., Nicol, D., and Clements, J. (1999). The human tissue kallikreins (KLK1–3) and a novel KLK1 mRNA transcript are expressed in a renal cell carcinoma cDNA library. Immunopharmacology45, 83–88.10.1016/S0162-3109(99)00059-4Suche in Google Scholar
Ramsaroop, R., Naicker, S., Naicker, T., Naidoo, S., and Bhoola, K.D. (1997). Tissue kallikrein and kinins in renal disease. Immunopharmacology36, 255–261.10.1016/S0162-3109(97)00030-1Suche in Google Scholar
Rittenhouse, H.G., Finlay, J.A., Mikolajczyk, S.D., and Partin, A.W. (1998). Human kallikrein 2 (hK2) and prostate-specific antigen (PSA): two closely related, but distinct, kallikreins in the prostate. Crit. Rev. Clin. Lab. Sci.35, 275–368.10.1080/10408369891234219Suche in Google Scholar
Seidah, N.G. and Chretien, M. (1999). Proprotein and prohormone convertases: a family of subtilases generating diverse bioactive peptides. Brain Res.848, 45–62.10.1016/S0006-8993(99)01909-5Suche in Google Scholar
Shigemasa, K., Tian, X., Gu, L., Tanimoto, H., Underwood, L.J., O'Brien, T.J., and Ohama, K. (2004). Human kallikrein 8 (hK8/TADG-14) expression is associated with an early clinical stage and favourable prognosis in ovarian cancer. Oncol. Rep.11, 1153–1159.Suche in Google Scholar
Shimizu-Okabe, C., Yousef, G.M., Diamandis, E.P., Yoshida, S., Shiosaka, S., and Fahnestock, M. (2001). Expression of the kallikrein gene family in normal and Alzheimer's disease brain. Neuroreport12, 2747–2751.10.1097/00001756-200108280-00031Suche in Google Scholar
Shvartsman, H.S., Lu, K.H., Lee, J., Lillie, J., Deavers, M.T., Clifford, S., Wolf, J.K., Mills, G.B., Bast, R.C., Gershenson, D.M., and Schmandt, R. (2003). Overexpression of kallikrein 10 in epithelial ovarian carcinomas. Gynecol. Oncol.90, 44–50.10.1016/S0090-8258(03)00257-9Suche in Google Scholar
Siivola, P., Pettersson, K., Piironen, T., Lovgren, T., Lilja, H., and Bjartell, A. (2000). Time-resolved fluorescence imaging for specific and quantitative immunodetection of human kallikrein 2 and prostate-specific antigen in prostatic tissue sections. Urology56, 682–68810.1016/S0090-4295(00)00671-3Suche in Google Scholar
Simmer, P.J. (2004). Kallikrein 4 is a secreted protein. Cancer Res.64, 8481–8483.10.1158/0008-5472.CAN-04-1660Suche in Google Scholar PubMed
Stenman, U.-H. (1999). New ultrasensitive assays facilitate studies on the role of human glandular kallikrein (hK2) as a marker for prostatic disease. Clin. Chem.45, 753–754.10.1093/clinchem/45.6.753Suche in Google Scholar
Stephan, C., Yousef, G.M., Scorilas, A., Jung, K., Jung, M., Kristiansen, G., Hauptmann, S., Bharaj, B.S., Nakamura, T., Loening, S.A., and Diamandis, E.P. (2003). Quantitative analysis of kallikrein 15 gene expression in prostate tissue. J. Urol.169, 361–364.10.1097/00005392-200301000-00107Suche in Google Scholar
Tanimoto, H., Underwood, L.J., Shigemasa, K., Yan Yan, M.S., Clarke, J., Parmley, T.H., and O'Brien, T.J. (1999). The stratum corneum chymotryptic enzyme that mediates shedding and desquamation of skin cells is highly overexpressed in ovarian tumor cells. Cancer86, 2074–2782.10.1002/(SICI)1097-0142(19991115)86:10<2074::AID-CNCR27>3.0.CO;2-3Suche in Google Scholar
Terashima, H., Atomi, Y., Kuroda, A., Morioka, Y., Ikekita, M., Aoki, K., Kamada, M., Kizuki, K., and Moriya, H. (1989). Purification of human pancreatic kallikrein and organ-specificities of human glandular kallikrein [in Japanese]. Nippon Shokakibyo Gakkai Zasshi86, 2556–2565.Suche in Google Scholar
Terayama, R., Bando, Y., Takahashi, T., and Yoshida, S. (2004). Differential expression of neuropsin and protease M/neurosin in oligodendrocytes after injury to the spinal cord. Glia48, 91–101.10.1002/glia.20058Suche in Google Scholar
Tremblay, R.R., Deperthes, D., Tetu, B., and Dube, J.Y. (1997). Immunohistochemical study suggesting a complementary role of kallikreins hK2 and hK3 (prostate-specific antigen) in the functional analysis of human prostate tumors. Am. J. Pathol.150, 455–459.Suche in Google Scholar
Uehara, S., Honjyo, K., Furukawa, S., Hirayama, A., and Sakamoto, W. (1989). Role of the kallikrein-kinin system in human pancreatitis. Adv. Exp. Med. Biol.247B, 643–648.10.1007/978-1-4615-9546-5_106Suche in Google Scholar
Underwood, L.J., Tanimoto, H., Wang, Y., Shigemasa, K., Parmley, K., and O'Brien, T.J. (1999). Cloning of tumor-associated differentially expressed gene-14, a novel serine protease overexpressed by ovarian carcinoma. Cancer Res.59, 4435–4439.Suche in Google Scholar
Vio, C.P., Roa, J.P., Silva, R., and Powers, C.A. (1990). Localization of immunoreactive glandular kallikrein in lactotrophs of the rat anterior pituitary. Neuroendocrinology51, 10–14.10.1159/000125309Suche in Google Scholar
Williams, R.J., Henderson, L.M., Naidoo, Y., Cassim, B., Elson, C.J., and Bhoola, K.D. (1997). Immunocytochemical analysis of tissue kallikrein and the kinin moiety in rheumatoid synovial fluid neutrophils. Br. J. Rheumatol.36, 420–425.10.1093/rheumatology/36.4.420Suche in Google Scholar
Wolf, W.C., Harley, R.A., Sluce, D., Chao, L., and Chao, J. (1999). Localization and expression of tissue kallikrein and kallistatin in human blood vessels. J. Histochem. Cytochem.47, 221–228.10.1177/002215549904700210Suche in Google Scholar
Xi, Z., Klokk, T.I., Korkmaz, K., Kurys, P., Elbi, C., Risberg, B., Danielsen, H., Loda, M., and Saatcioglu, F. (2004). Kallikrein 4 is a predominantly nuclear protein and is overexpressed in prostate cancer. Cancer Res.64, 2365–2370.10.1158/0008-5472.CAN-03-2025Suche in Google Scholar
Yamanaka, H., He, X., Matsumoto, K., Shiosaka, S., and Yoshida, S. (1999). Protease M/neurosin mRNA is expressed in mature oligodendrocytes. Brain Res. Mol. Brain Res.71, 217–224.10.1016/S0169-328X(99)00187-4Suche in Google Scholar
Yayama, K., Kunimatsu, N., Teranishi, Y., Takano, M., and Okamoto, H. (2003). Tissue kallikrein is synthesized and secreted by human vascular endothelial cells. Biochem. Biophys. Acta1593, 231–238.10.1016/S0167-4889(02)00393-2Suche in Google Scholar
Yoi, O.O., Seldin, D.C., Spragg, J., Pinkus, G.S., and Austen, K.F. (1979). Sequential cleavage of proinsulin by human pancreatic kallikrein and a human pancreatic kininase. Proc. Natl. Acad. Sci. USA76, 3612–3616.Suche in Google Scholar
Yousef, G.M. and Diamandis, E.P. (2001). The new human tissue kallikrein gene family: structure, function, and association to disease. Endocr. Rev.22, 184–204.Suche in Google Scholar
Yousef, G.M. and Diamandis, E.P. (2002). Human tissue kallikreins: a new enzymatic cascade pathway? Biol. Chem.383, 1045–1057.10.1515/BC.2002.113Suche in Google Scholar
Yousef, G.M., Scorilas, A., Magklara, A., Soosaipillai, A., and Diamandis, E.P. (2000). The KLK7 (PRSS6) gene, encoding for the stratum corneum chymotryptic enzyme is a new member of the human kallikrein gene family – genomic characterization, mapping, tissue expression and hormonal regulation. Gene254, 119–128.10.1016/S0378-1119(00)00280-8Suche in Google Scholar
Yousef, G.M., Magklara, A., Chang, A., Jung, K., Katsaros, D., and Diamandis, E.P. (2001). Cloning of a new member of the human kallikrein gene family, KLK14, which is down-regulated in different malignancies. Cancer Res.61, 3425–3431.Suche in Google Scholar
Yousef, G.M., Borgono, C.A., Scorilas, A., Ponzone, R., Biglia, N., Iskander, L., Polymeris, M.E., Roagna, R., Sismondi, P., and Diamandis, E.P. (2002a). Quantitative analysis of human kallikrein gene 14 expression in breast tumours indicates association with poor prognosis. Br. J. Cancer87, 1287–1293.10.1038/sj.bjc.6600623Suche in Google Scholar
Yousef, G.M., Obiezu, C.V., Jung, K., Stephan, C., Scorilas, A., and Diamandis, E.P. (2002b). Differential expression of kallikrein gene 5 in cancerous and normal testicular tissues. Urology60, 714–718.10.1016/S0090-4295(02)01811-3Suche in Google Scholar
Yousef, G.M., Scorilas, A., Chang, A., Rendl, L., Diamandis, M., Jung, K., and Diamandis, E.P. (2002c). Down-regulation of the human kallikrein gene 5 (KLK5) in prostate cancer tissues. Prostate51, 126–132.10.1002/pros.10067Suche in Google Scholar
Yousef, G.M., Scorilas, A., Kyriakopoulou, L.G., Rendl, L., Diamandis, M., Ponzone, R., Biglia, N., Gial, M., Roagna, R., Sismondi, P., and Diamandis, E.P. (2002d). Human kallikrein gene 5 (KLK5) expression by quantitative PCR: an independent indicator of poor prognosis in breast cancer. Clin. Chem.48, 1241–1250.10.1093/clinchem/48.8.1241Suche in Google Scholar
Yousef, G.M., Scorilas, A., Magklara, A., Memari, N., Ponzone, R., Sismondi, P., Biglia, N., Abd Ellatif, M., and Diamandis, E.P. (2002e). The androgen-regulated gene human kallikrein 15 (KLK15) is an independent and favourable prognostic marker for breast cancer. Br. J. Cancer87, 1294–1300.10.1038/sj.bjc.6600590Suche in Google Scholar
Yousef, G.M., Kishi, T., and Diamandis, E.P. (2003a). Role of kallikrein enzymes in the central nervous system. Clin. Chim. Acta329, 1–8.10.1016/S0009-8981(03)00004-4Suche in Google Scholar
Yousef, G.M., Polymeris, M., Yacoub, G.M., Scorilas, A., Soosaipaillai, A., Popalis, C., Fracchioli, S., Katsaros, D., and Diamandis, E.P. (2003b). Parallel overexpression of seven kallikrein genes in ovarian cancer. Cancer Res.63, 2223–2227.Suche in Google Scholar
Yousef, G.M., Scorilas, A., Katsaros, D., Fracchioli, S., Iskander, L., Borgono, C., Rigault de la Longrais, I.A., Puopolo, M., Massobrio, M., and Diamandis, E.P. (2003c). Prognostic value of the human kallikrein gene 15 expression in ovarian cancer. J. Clin. Oncol.21, 3119–3126.10.1200/JCO.2003.09.111Suche in Google Scholar PubMed
Yousef, G.M., Scorilas, A., Nakamura, T., Ellatif, M.A., Ponzone, R., Biglia, N., Maggiorotto, F., Roagna, R., Sismondi, P., and Diamandis, E.P. (2003d). The prognostic value of the human kallikrein gene 9 (KLK9) in breast cancer. Breast Cancer Res. Treat.78, 149–158.10.1023/A:1022931403825Suche in Google Scholar
Yousef, G.M., Obiezu, C.V., Luo, L.Y., Magklara, A., Borgono, C.A., Kishi, T., Memari, N., Michael, P., Sidiropoulos, M., Kurlender, L., et al. (2005). Human tissue kallikreins: from gene structure to function and clinical applications. Adv. Clin. Chem.39, 11–79.10.1016/S0065-2423(04)39002-5Suche in Google Scholar
©2006 by Walter de Gruyter Berlin New York
Artikel in diesem Heft
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)
Artikel in diesem Heft
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)