Fast Control of DNA Replication in Response to Hypoxia and to Inhibited Protein Synthesis in CCRF-CEM and HeLa Cells
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Gudrun Probst
Abstract
In order to elucidate whether data about the fast regulation of DNA replication in dependence on oxygen supply and on a functioning protein synthesis, previously elaborated with Ehrlich ascites cells, are valid for human cells too, we repeated key experiments with CCRF-CEM and HeLa cells. The most important techniques employed were DNA fibre autoradiography and alkaline sedimentation analyses of growing (pulselabeled) daughter strand DNA. It was found that CCRF-CEM and HeLa cells responded to transient hypoxia and to transient inhibition of protein synthesis in an almost identical fashion. Scheduled replicon initiations were reversibly suppressed and the progress rates of replication forks, which were already active before the respective inhibitory conditions were established, were reversibly slowed down. The inclusion of the fork progress rate in the response differs from Ehrlich ascites cells, which respond only by suppressing initiation. Further circumstances of the fast oxygen dependent response, concerning the behaviour of ribonucleotide reductase and of the dNTP pools, revealed no significant differences among the three cell lines. The striking identity of the response of each of the cell lines to hypoxia and to inhibited protein synthesis prompts the suspicion that converging fast regulatory pathways act on the cellular replication machinery. The phenomena as such seem to be rather widespread among mammalian cells.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Artikel in diesem Heft
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- Editor's Note
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- Molecular Genetics of Dopa-Responsive Dystonia
- In Vitro Transcription of a TATA-Less Promoter: Negative Regulation by the Not1 Protein
- Fast Control of DNA Replication in Response to Hypoxia and to Inhibited Protein Synthesis in CCRF-CEM and HeLa Cells
- The Inhibition of NF-B Activation Pathways and the Induction of Apoptosis by Dithiocarbamates in T Cells Are Blocked by the Glutathione Precursor N-Acetyl-L-Cysteine
- Xylose Utilisation: Cloning and Characterisation of the Xylose Reductase from Candida tenuis
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- Comparison of the Tamoxifen Regulated Chimeric Cre Recombinases MerCreMer and CreMer
- The Human Cathepsin F Gene a Fusion Product between an Ancestral Cathepsin and Cystatin Gene
- Characterization of a New Member of the TNF Family Expressed on Antigen Presenting Cells
- Vascular Endothelial Growth Factor (VEGF) and Its Receptors in Tumor-Bearing Dogs
- Molecular Cloning and Characterization of a cDNA Encoding a Transferrin Homolog from Bombyx mori
- Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation
- Acknowledgement
- Content Index
- Author Index
- Subject Index
Artikel in diesem Heft
- To Our Authors, Readers and Subscribers
- Editor's Note
- A Chimpanzee Millennium
- Molecular Genetics of Dopa-Responsive Dystonia
- In Vitro Transcription of a TATA-Less Promoter: Negative Regulation by the Not1 Protein
- Fast Control of DNA Replication in Response to Hypoxia and to Inhibited Protein Synthesis in CCRF-CEM and HeLa Cells
- The Inhibition of NF-B Activation Pathways and the Induction of Apoptosis by Dithiocarbamates in T Cells Are Blocked by the Glutathione Precursor N-Acetyl-L-Cysteine
- Xylose Utilisation: Cloning and Characterisation of the Xylose Reductase from Candida tenuis
- Xylose Utilisation: Cloning and Characterisation of the Xylitol Dehydrogenase from Galactocandida mastotermitis
- Thermodynamic Properties and DNA Binding of the ParD Protein from the Broad Host-Range Plasmid RK2/RP4 Killing System
- Dipeptidyl Peptidase III from Rat Liver Cytosol: Purification, Molecular Cloning and Immunohistochemical Localization
- Genomic Expansion Across the Albumin Gene Family on Human Chromosome 4q Is Directional
- Comparison of the Tamoxifen Regulated Chimeric Cre Recombinases MerCreMer and CreMer
- The Human Cathepsin F Gene a Fusion Product between an Ancestral Cathepsin and Cystatin Gene
- Characterization of a New Member of the TNF Family Expressed on Antigen Presenting Cells
- Vascular Endothelial Growth Factor (VEGF) and Its Receptors in Tumor-Bearing Dogs
- Molecular Cloning and Characterization of a cDNA Encoding a Transferrin Homolog from Bombyx mori
- Mitochondria-Derived and Extra-Mitochondrial Human Type-1 Porin Are Identical as Revealed by Amino Acid Sequencing and Electrophysiological Characterisation
- Acknowledgement
- Content Index
- Author Index
- Subject Index
