Total Antioxidant Capacity of Feces of Mammalian Herbivores and Carnivores
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Bogdan Saletnik
The total antioxidant capacities (TAC) of feces of mammalian herbivores and carnivores were compared. TAC were estimated using three different methods: 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) radical (ABTS·) reduction, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) reduction, and ferric reducing antioxidant power (FRAP). TAC of 18 herbivorous species were generally higher with respect to 16 carnivorous species [(14:21±6:72) vs. (9:45±7:32) mmol Trolox equivalents/kg feces; P < 0:05] in the FRAP assay. The ABTS· reduction assay indicated that the TAC originating from “fast” reacting antioxidants were higher in the herbivores than in carnivores [(17:92±7:18) vs. (12:22±8:5) mmol Trolox equivalents/kg feces; P < 0:05], while a reverse trend was observed for TAC originating from “slowly” reacting antioxidants [(20:68±4:85) vs. (24:68±6:87) mmol Trolox equivalents/kg feces].
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