Optimization of ammonium nitrate concentration in single-stage continuous cultures of Aspergillus niger with biomass retention
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M. Janczar
und
J. Pietkiewicz
Abstract
The effect of ammonium nitrate concentration in the citric acid biosynthesis by Aspergillus niger NC-12 in single-stage continuous cultures with biomass retention was investigated. Experiments were carried out in a BIOMER laboratory fermenter with 5 dm3 working volume. At the initial stage of each cultivation, the substrate in the bioreactor contained 1.5 g NH4NO3 dm−3. After 120 h onwards, the bioreactor was fed continuously at a constant dilution rate of 0.009 h−1. NH4NO3 concentration in the feed was varied from one culture to another, ranging between 0.5 g dm−3 and 2.5 g dm−3. Promising results were obtained when NH4NO3 concentration of 1.5 g dm−3 was used. The observed concentration of citric acid (c P) and yield of citric acid with respect to the introduced sucrose (Y P/S) were 117.88 g dm−3 and 78.59 %, respectively. The efficiency coefficient of citric acid biosynthesis (K ef) was very high, amounting to 83.38.
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© 2007 Institute of Chemistry, Slovak Academy of Sciences
Artikel in diesem Heft
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Artikel in diesem Heft
- A review of methods for synthesis of nanostructured metals with emphasis on iron compounds
- Effect of selenium oxidation state on cadmium translocation in chamomile plants
- Optimization of ammonium nitrate concentration in single-stage continuous cultures of Aspergillus niger with biomass retention
- Behaviour of inorganic constituents of municipal sewage sludge during fluidized-bed combustion
- Profile distribution of As(III) and As(V) species in soil and groundwater in Bozanta area
- Structure of cyano-bridged Eu(III)—Co(III) bimetallic assembly and its application to photophysical verification of photomagnetic phenomenon
- Synthesis and characterization of oligo-4-[(pyridin-3-ylimino)methyl]phenol
- Relationship between physicochemical properties, lipophilicity parameters, and local anesthetic activity of dibasic esters of phenylcarbamic acid
- Improved DPPH determination for antioxidant activity spectrophotometric assay
- Notes on notation of sodium oxofluoroaluminate anions
- Determination of the enthalpy of fusion of Na3FeF6
- Controlled solvothermal synthesis of PbS quasi-nanorods by calix[4]arene
- Palladium-catalyzed heck and suzuki coupling in glycerol
- Synthesis of new condensed and cyclized coumarin derivatives
- M. Hartman, O. Trnka, and M. Pohořelý: Fluidization behavior of oil-contaminated sand
