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Isolation of Immunoglobulin from Chicken Egg Yolk using Single-Stage Ultrafiltration with 100-kDa Regenerated Cellulose Membranes
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Jianguo Liu
Veröffentlicht/Copyright:
11. Januar 2011
The aim of this work is to develop a membrane-based cost-effective process for the rapid isolation of immunoglobulin from chicken egg yolk. It was found that a single-stage ultrafiltration using a 100 kDa molecular weight cut-off regenerated cellulose membrane could be employed to isolate immunoglobulin from the crude feedstock. The effects of operational parameters (solution pH, ionic strength, stirring speed and permeate flux) on the transmission of immunoglobulin and the presence of impurity protein with molecular weight close to immunoglobulin were quantified using the parameter scanning ultrafiltration technique. Under optimized conditions, the purity of immunoglobulin obtained was about 85 percent after the single-stage ultrafiltration process, and the recovery of immunoglobulin from the feedstock was 91 percent.
Published Online: 2011-1-11
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Artikel in diesem Heft
- Article
- Improving Anti-?-Glucosidase Activity of Douchi Koji Using a Newly Isolated Strain of Bacillus subtilis B2
- Different Approaches for Mass Transfer Studies on Potato Cylinders and Slices during Solar Drying
- Effects of Different Temperatures on Storage Quality of Heavily Salted Cod (Gadus morhua)
- Evaluation of Moisture Diffusion Coefficient of Cylindrical Bodies Considering Shrinkage During Natural Convection Drying
- Effect of Raw Material and Processing Factors on the Production of Effervescent Artichoke (Cynara scolymus L.) Tea Tablets
- Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris
- Suitability of Feature Extraction Methods in Recognition and Classification of Grains, Fruits and Flowers
- Hydrodistillation of Essential Oil from Cymbopogon flexuosus
- A Survey on Packaging Materials and Technologies for Commercial Food Products
- Formulation, Drying and Nutritional Evaluation of Ready-to-Eat Sapota (Achras zapota) Extrudes
- High Hydrostatic Pressure Effect on Natural Microflora, Saccharomyces cerevisiae, Escherichia coli, and Listeria Innocua in Navel Orange Juice
- Effective Parameters Consideration in Ohmic Heating Process in Two Phase Static System of Bio-Particle-Liquid
- Proximate Composition of Dry Fermented Turkish Sausage (Sucuk) as Affected by Ripening Period, Nitrite Level and Heat Treatment
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- Relative Antioxidant Activity of Common Indian Herbs
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