Bioconversion of De-Oiled Rice Bran (DORB) Hemicellulosic Hydrolysate into Ethanol by Pichia stipitis NCM3499 under Optimized Conditions
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Anuj Kumar Chandel
In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Artikel in diesem Heft
- Article
- Flow Behaviour of Black Chokeberry (Aronia melanocarpa) Juice
- Use of an Enzymatic Process for Extracting and Hydrolyzing Rice Proteins Aiming at Phenylalanine Removal
- CFD Modeling of Heat Transfer Augmentation in a Circular Tube Fitted with Right-Left Helical Inserts in Turbulent Flow
- Studies on the Production of Acid Protease by Submerged Fermentation
- Bioconversion of De-Oiled Rice Bran (DORB) Hemicellulosic Hydrolysate into Ethanol by Pichia stipitis NCM3499 under Optimized Conditions
- Extrusion Processing of Starch Film
- Optimization of a Convective Air Flow Solar Food Dryer
- Influence of Cryogenic Grinding on Release of Protein and DNA from Saccharomyces cerevisiae
- Lipase-Catalyzed Acyl-L-Carnitines Synthesis in [Bmim]PF6 Ionic Liquid
- Discriminating Structural Characteristics of Starch Extrudates through X-ray Micro-tomography using a 3-D Watershed Algorithm
- Shorter Communication
- Effects of Engineering Properties on the Poppability of Nigerian Popcorn
