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Concerns About Unreliable Data from Spotted cDNA Microarrays Due to Cross-Hybridization and Sequence Errors
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Daniel Handley
, Nicoleta Serban , David G Peters and Clark Glymour
Published/Copyright:
October 6, 2004
We discuss our concerns regarding the reliability of data generated by spotted cDNA microarrays. Two types of error we highlight are cross-hybridization artifact due to sequence homologies and sequence errors in the cDNA used for spotting on microarrays. We feel that statisticians who analyze microarray data should be aware of these sources of unreliability intrinsic to cDNA microarray design and use.
Published Online: 2004-10-6
©2011 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
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Keywords for this article
Cross-hybridization;
Sequence Errors;
cDNA microarray;
Sources of Error
Articles in the same Issue
- Article
- Using Alpha Wisely: Improving Power to Detect Multiple QTL
- Relating HIV-1 Sequence Variation to Replication Capacity via Trees and Forests
- Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments
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- Using Importance Sampling to Improve Simulation in Linkage Analysis
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- Multiple Testing. Part II. Step-Down Procedures for Control of the Family-Wise Error Rate
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