99mTc-Novobiocin: a~novel radiotracer for infection imaging
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S. Q. Shah
Abstract
The radiosynthesis of 99mTc-Novobiocin (99mTc-NBN) complex and its suitability as a radiotracer for infection imaging was assessed. The radiochemical purity (RCP) of the 99mTc-NBN complex was determined using radio-TLC and radioactive HPLC and biodistribution was studied in artificially infected (A.I.) rats and rabbit, using single well gamma counter (SWGRC) interface with scalar count rate meter (SCRM) and Gamma Camera (γ-CM). The maximum RCP observed for the preparation having 2 mg of NBN, 111 MBq of sodium pertechnetate (Na99mTcO4) and 125 μL of SnF2 (1 μg/μL in 0.01 N HCl) at a pH 5.6 was 98.97±0.40% and remained stable >90% up to 120 min. The activity of the 99mTc-NBN in the infected muscle (TI) was significantly increased from 6.50±0.15 to 19.00±0.17% and decreased in the inflamed muscle (TII), normal muscle (NT), blood, liver, spleen, stomach and intestine within 120 min. The TI/NT and TII/NT ratios were 7.60±1.08 and 1.60±1.14. The Whole Body Static (WBS) images of A.I. rabbit were obtained at 30, 40, 50 and 60 min after the I.V. administration of 111 MBq of 99mTc-NBN to the A.I. rabbit. The stability in saline and serum, higher TI/NT, lower TII/NT ratios and WBS images confirmed the feasibility of the 99mTc-NBN complex as an infection imaging agent.
© by Oldenbourg Wissenschaftsverlag, Peshawar, NWFP, Germany
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Articles in the same Issue
- Excitation functions and yields for cyclotron production of radiorhenium vianat W(p,ߙxn)181-186gRe nuclear reactions and tests on the production of 186gRe using enriched 186W
- Optimising BTP ligands by tuning their basicity
- Investigations on redox behaviour of Pu(IV)/Pu(III) in H2SO4 on Pt nanoparticles-modified glassy carbon and platinum electrodes
- Destruction of soluble organics generated during the dissolution of sintered uranium carbide by mediated electrochemical oxidation process
- Complexation of thorium with phosphate
- Sorption of radiocesium from liquid radioactive waste on clay and immobilization by baking the clay at elevated temperature
- Comparison of different phosphorus-containing ligands complexing 68Ga for PET-imaging of bone metabolism
- 99mTc-Novobiocin: a~novel radiotracer for infection imaging
- Determination of the activation constants for miniature neutron source reactor (MNSR) by linear least squares regression
