Preliminary speciation study of Co(II)-citrate system by ion pairing-reversed phase chromatography coupled to UV detector
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C. Bresson
Summary
This paper describes the ability of ion pairing-reversed phase chromatography coupled to UV detector to determine an experimental speciation of cobalt in the binary Co(II)-citrate system. A method has been developed to investigate aqueous Co(II)-citrate samples at physiological pH as a function of time, by varying metal-to-ligand ratios, concentration and temperature. Solid 1:1 dimeric and 1:2 monomeric Co(II)-citrate complexes previously described have been synthesised and their analysis by IP-RPC-UV allowed the assignation of the solution state separated species. In solution state and at Co(II) concentration of 5×10-2 M, 1:2 Co(II)-citrate monomeric species are predominant forms, while 1:1 mono and dimeric complexes are the minor species. At Co(II) concentration lower than 5 mM, only species of 1:1 stoichiometry are involved in the aqueous cobalt distribution. The monitoring of the formed species as a function of time allows highlighting the high inertness of this system. At low concentration ranges, electrospray ionisation-mass spectrometry experiments were carried out to support the chromatographic data. This study is the first one establishing a link among synthetic cobalt-citrate complexes and species existing in solution.
© Oldenbourg Wissenschaftsverlag, München
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Articles in the same Issue
- Preface: Chemical Speciation
- Transport methods as complementary tools for speciation purposes
- Contribution of hyphenated CE-ICP/MS in metal/protein interactions studies
- Interfacing capillary electrophoresis with inductively coupled plasma mass spectrometry for redox speciation of plutonium
- Preliminary speciation study of Co(II)-citrate system by ion pairing-reversed phase chromatography coupled to UV detector
- Resistance, accumulation and transformation of selenium by the cyanobacterium Synechocystis sp. PCC 6803 after exposure to inorganic SeVI or SeIV
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- Actinide uptake by transferrin and ferritin metalloproteins
- Plutonium speciation affected by environmental bacteria
- Influence of thermodynamic database on the modelisation of americium(III) speciation in a simulated biological medium
- The OECD/NEA TDB review of selected organic ligands
