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Effect of ionic strength on DNA–dye interactions of Victoria blue B and methylene green using UV–visible spectroscopy

  • Faizan Ur Rahman , Shahab Khan , Maooz Ur Rahman , Rukhsana Zaib , Mudassir Ur Rahman , Riaz Ullah , Muhammad Zahoor EMAIL logo and Abdul Waheed Kamran
Published/Copyright: November 20, 2023
Zeitschrift für Physikalische Chemie
From the journal Zeitschrift für Physikalische Chemie Volume 238 Issue 1

Abstract

Victoria blue and methylene green dyes have both been extensively studied due to their numerous applications, including their ability to bind to DNA. Dyes are very important in everyday life with applications in textile, cosmetics, food and pharmaceutical industries. It has been found that some of them adversely affect human health causing severe abnormalities. Among these abnormalities, cancer is of great concern due to its fatal and almost non-recoverable nature. In this work we have studied the binding of two dyes namely Victoria blue B (VBB) and Methylene green (MG) with double stranded DNA (Salmon sperm). The interactions were studied in the presence of different concentrations of buffer solutions at a constant pH. The selected dyes showed interactions with double-stranded DNA through intercalation and electrostatic modes. Upon increasing ionic strength of the buffer the binding constant (K b ) value for MG was decreased whereas increased for VBB, which conclude that, at higher ionic strength (0.5 M) the DNA–MG interactions is lower and DNA–VVB interactions is maximum. The carcinogenicity of a given dye is indicated from its binding constants in the current study. Based on the recorded K b values of the selected dyes it was concluded that proper disposing and precautions should be taken while utilizing/dealing these dyes in order to minimize/avoid their impact on environment and human health.

Keywords: Victoria blue B; methylene green; UV–visible spectroscopy; buffer solution
Corresponding author: Muhammad Zahoor, Department of Biochemistry, University of Malakand, 18800 Chakdara, Dir (Lower), Khyber Pakhtunkhwa, Pakistan, E-mail:

Funding source: King Saud University

Award Identifier / Grant number: Unassigned

Acknowledgment

The authors extend their appreciation to the researchers supporting Project number (RSP2023R110) King Saud University, Riyadh, Saudi Arabia, for financial support.

  1. Research ethics: Not applicable.

  2. Author contributions: Faizan Ur Rahman and Shahab Khan prepared DNA for processing and design scheme of the study, Maooz Ur Rahman and Rukhsana Zaib interpreted the data, Mudassir Ur Rahman and R. Ullah performed validation of the data, A. W. Kamran and M. Zahoor performed UV-visible spectrum of Victoria Blue B and Methylene Green and wrote the paper and revised.

  3. Competing interests: The authors declares that they have no competing interest.

  4. Research funding: This work was supported by King Saud University, researchers supporting Project (number RSP2023R110), King Saud University, Riyadh, Saudi Arabia.

  5. Data availability: No data is associated with this pubication.

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Received: 2023-09-26
Accepted: 2023-11-05
Published Online: 2023-11-20
Published in Print: 2024-01-29

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Review Article
  3. Potential of Gd-based nanocomposites (GdFeO3) as photocatalysts for the degradation of organic pollutants: a review
  4. Original Papers
  5. Bimetallic nanoparticles preparation from metallic organic frameworks, characterization and its applications in reclamation of textile effluents
  6. Chitosan-coated magnetic nanorods and nanospheres: physicochemical characterizations and potential as methotrexate carriers for targeted drug delivery
  7. Green synthesis of copper nanoparticles from agro-waste garlic husk
  8. Noncovalent interactions in N-methylurea crystalline hydrates
  9. Upcycling of the industrial waste as a sustainable source of axenic fungal strain (Aspergillus oryzae) for scale up enzymatic production with kinetic analysis and Box–Behnken design application
  10. Kinetics and outer sphere electron transfer of some metallosurfactants by Fe(CN)64− in microheterogenous medium: a detailed thermodynamic approach
  11. Bonding and noncovalent interactions effects in 2,6-dimethylpiperazine-1,4-diium oxalate oxalic acid: DFT calculation, topological analysis, NMR and molecular docking studies
  12. Effect of ionic strength on DNA–dye interactions of Victoria blue B and methylene green using UV–visible spectroscopy
  13. Synthesis, X-ray diffraction, DFT, and molecular docking studies of isonicotinohydrazide derivative
https://doi.org/10.1515/zpch-2023-0365
Keywords for this article
Victoria blue B; methylene green; UV–visible spectroscopy; buffer solution

Articles in the same Issue

  1. Frontmatter
  2. Review Article
  3. Potential of Gd-based nanocomposites (GdFeO3) as photocatalysts for the degradation of organic pollutants: a review
  4. Original Papers
  5. Bimetallic nanoparticles preparation from metallic organic frameworks, characterization and its applications in reclamation of textile effluents
  6. Chitosan-coated magnetic nanorods and nanospheres: physicochemical characterizations and potential as methotrexate carriers for targeted drug delivery
  7. Green synthesis of copper nanoparticles from agro-waste garlic husk
  8. Noncovalent interactions in N-methylurea crystalline hydrates
  9. Upcycling of the industrial waste as a sustainable source of axenic fungal strain (Aspergillus oryzae) for scale up enzymatic production with kinetic analysis and Box–Behnken design application
  10. Kinetics and outer sphere electron transfer of some metallosurfactants by Fe(CN)64− in microheterogenous medium: a detailed thermodynamic approach
  11. Bonding and noncovalent interactions effects in 2,6-dimethylpiperazine-1,4-diium oxalate oxalic acid: DFT calculation, topological analysis, NMR and molecular docking studies
  12. Effect of ionic strength on DNA–dye interactions of Victoria blue B and methylene green using UV–visible spectroscopy
  13. Synthesis, X-ray diffraction, DFT, and molecular docking studies of isonicotinohydrazide derivative
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