Anti-dengue fever activity of a polyherbal extract from medicinal plants of Vietnam
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Vo Hoai Bac
Abstract
This study investigated the anti - dengue hemorrhagic fever (DHF) and anti-dengue virus (DENV) properties of an extract containing six Vietnamese medicinal plants (HemoShield – HE). HE in water (WE) and in 25 % ethanol (EE) exhibited anti-DENV activity against all four tested DENV serotypes (1, 2, 3, and 4), with the strongest effect observed with DENV2. Moreover, HE demonstrated apparent inhibitory activity against NS2B-NS3pro enzyme responsible for viral replication with an IC50 value of 48.5 ± 2.3 μg/mL. In the thrombocytopenia rats, HE-treated group showed a significant increase in platelet count (approximately 33.62 %; p < 0.05). Additionally, the bleeding time of 0.74 g/kg treatment group was significantly reduced by 33.4 % (p < 0.05). Antipyretic activity of HE in rabbits after 3-h treatment showed a significant reduction in body temperature (38.8 %; ΔT: 0.4 ± 0.1 °C) at a dose of 0.31 g/kg. Thus, our findings provided new in vitro and in vivo evidence demonstrating the effect of HE in alleviating DHF.
Funding source: The VPO-PHARCO Company-Vietnam
Award Identifier / Grant number: VPO/RG-2023-01
Acknowledgments
We thank Dr. Ngo Van Quang, Institute of Chemistry, Vietnam Academy of Science and Technology for his help in HPLC analyses.
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Research ethics: The research meets the ethical guidelines, including adherence to the legal requirements of the study country. Research ethics was approved by the Animal Ethics Committee of National Institute of Medicinal Materials (NIMM), Ministry of Health, Vietnam (Decision No.01/2023-HDTVEETDV dated 12th August, 2023).
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Informed consent: Not applicable.
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Author contributions: NTMP, VHB contributed to Conceptualization, Methodology, Supervision, Formal Analysis, Writing-Original Draft/Review & Editing and Project Administration. DK, DVQ contributed to Writing-Review & Editing. LTX, NTTT, VTBH, HK, NTPT contributed Wetlab Assessments, Formal Analysis, Validation, Writing-Original Draft. NVD and NVT provided Hemoshield product, contributed to Discussion and Review. All authors have read and agreed to the submitted version of the manuscript.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The polyherbal extract Hemoshield was produced by VPO JSC., in which NVD and NVT are founders and directors. The remained authors report no declarations of interest.
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Research funding: This work was supported by the VPO-PHARCO Company-Vietnam under grant No. VPO/RG-2023-01.
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Data availability: The supplementary data on Analyses of HPLC chromatogram of Apigenin, Caffeic acid, Luteolin, Quercetin, Astilbin used to support the findings of this study are available from the corresponding author upon request.
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Supplementary Material
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