Cardioprotective effects of omega 3 fatty acids from fish oil and it enhances autoimmunity in porcine cardiac myosin-induced myocarditis in the rat model

Ling-Yan Li; Xu Wang; Ting-Chuan Zhang; Zong-Jun Liu; Jun-Qing Gao

doi:10.1515/znc-2021-0057

Article

Cardioprotective effects of omega 3 fatty acids from fish oil and it enhances autoimmunity in porcine cardiac myosin-induced myocarditis in the rat model

Ling-Yan Li , Xu Wang , Ting-Chuan Zhang , Zong-Jun Liu and Jun-Qing Gao

Published/Copyright: May 31, 2021

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From the journal Zeitschrift für Naturforschung C Volume 76 Issue 9-10

Abstract

This experiment proposed to investigate the efficiency of omega 3 fatty acids from fish that improves autoimmune against myocarditis in the rat. Fish oil was extracted from fresh Tuna fish and performed FAME analysis and mice bioassay. The autoimmune myocarditis was induced by subcutaneous injection of porcine cardiac myosin (PCM) into the footpads of rats on the first and seventh day. Rats were dissected on the 21st day to analyze the histopathological, hemodynamic, echocardiographic factors, and immunohistochemistry expressions. In the study, 73.90% of total fatty acids were recorded. Histological analysis revealed that omega 3 fatty acids administrated groups showed tremendous development in the multifocal myocardia hyaline degeneration and necrosis with inflammatory changes. Moreover, omega 3 fatty acids inhabited the expressions of inflammatory cells (CD4, CD8 and CD11b) and suppressed the level of NF-κB. The echocardiographic factors such as heartbeat, SBP, DBP, levels of LVDs, LVDd, LVPW percentage of LVFS, EF, expression levels of inflammatory cytokines (TNF, IL-1β, IFN-ɤ, IL-2, and IL-6) also significantly suppressed by omega 3 fatty acids. Hence, the present study proved that consuming fatty acid-enriched fish might be a successful therapy for improving the inflammatory profile, regenerates the heart tissues, and controlled the production of inflammatory cells.

Keywords: autoimmune; cytokines; fatty acids; immunomodulatory; inflammatory; myocarditis

Corresponding author: Zong-Jun Liu and Jun-Qing Gao, Department of Cardiology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, No. 164 Lanxi Road, Shanghai 200062, People’s Republic of China, E-mail: lbjayk@163.com (Z.-J. Liu), Junqinggao73@gmail.com (J.-Q. Gao)

Funding source: Young Elite Scientists Medical Sponsorship Program

Award Identifier / Grant number: QNRC2-B03

Funding source: Clinical Advantage Discipline of Health System of Putuo District in Shanghai

Award Identifier / Grant number: 2019 Ysxk01

Funding source: Shanghai Health and Family Planning Medical Clinical Special Project

Award Identifier / Grant number: 201840247

Funding source: Shanghai Traditional Chinese Medicine Inheritance and technological innovation Project

Award Identifier / Grant number: Zycc2019026

Funding source: Shanghai Key Medical Specialties Construction Project

Award Identifier / Grant number: ZK2019A11

Acknowledgment

The authors are thankful to the higher authorities for the facilities provided.

Author contributions: This study was done by the authors named in this article, and the authors accept all liabilities resulting from claims which relate to this article and its contents.
Research funding: The Project was funded by The Overseas Program of Shanghai University of Chinese Medicine; The Shanghai Key Medical Specialties Construction Project (ZK2019A11); Shanghai Health and Family Planning Medical Clinical Special Project (201840247); Young Elite Scientists Medical Sponsorship Program (QNRC2-B03); Clinical Advantage Discipline of Health System of Putuo District in Shanghai (2019 Ysxk01); Shanghai Traditional Chinese Medicine Inheritance and technological innovation Project (Zycc2019026).
Conflict of interest: The author declares that no conflict of interest is associated with this study.

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Received: 2021-02-23

Accepted: 2021-05-08

Published Online: 2021-05-31

Published in Print: 2021-09-27

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Articles in the same Issue

https://doi.org/10.1515/znc-2021-0057

Keywords for this article

autoimmune; cytokines; fatty acids; immunomodulatory; inflammatory; myocarditis