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Evaluation of biological activities of Barbarea integrifolia and isolation of a new glucosinolate derivated compound

  • Merve Badem ORCID logo EMAIL logo , Sıla Ozlem Sener , Seyda Kanbolat , Nuriye Korkmaz , Sermet Yildirmiş , Ufuk Ozgen , Rezzan Aliyazicioglu , Emine Salva , Kübra Kaban , Ali Kandemir and İhsan Calıs ORCID logo
Published/Copyright: April 7, 2021
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 76 Issue 9-10

Abstract

The aim of the present study is to determine the potent biological activities and carry out isolation studies on Barbarea integrifolia. The antioxidant capacity of the species was evaluated by total phenolic content, FRAP, CUPRAC, and DPPH radical scavenging activity. Anticancer activity studies were performed by MTT assay in MDA-MB-231, MCF-7, Hep3B, PC-3, A549, HCT116, L-929 cell lines. It was observed that the remaining aqueous fraction has higher total phenolic content while higher activity in the CUPRAC and FRAP assays was displayed for the methanolic extract and chloroform fraction. The extracts showed anticancer activity as compared with vincristine. It was observed that chloroform fraction has the highest anticancer activity on MCF-7 cell line, while ethyl acetate fraction has the highest anticancer activity on Hep-3B and A549 cell lines. Methanolic extract has the highest anticancer activity on HCT116 and MDA-MB-23 cell lines. The isolation studies have been performed using several chromatographic methods. The chemical structures of compounds have been identified by means of 1H NMR, 13C NMR, 2D-NMR, and MS. Five major compounds, one steroid (β-Sitosterol), one phenolic acid (Rosmarinic acid), one flavonol heteroside (kaempferol 7-O-α-l-rhamnoside-3-O-β-d-(2-O-β- d -glucosyl)-β-d-glucoside), and two glucosinolates (Gluconasturtiin, Gluconasturtiin choline salt) have been isolated.

Keywords: anticancer activity; antioxidant; Barbarea ; Brassicaceae; gluconasturtiin choline salt
Corresponding author: Merve Badem, Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, 61080 Trabzon, Turkey, E-mail:

Funding source: Karadeniz Technical University Scientific Research Projects Unit

Award Identifier / Grant number: THD-2018-7353

Acknowledgments

Merve Badem, Sıla Özlem Şener and Nuriye Korkmaz would like to acknowledge the scholarship by the Scientific and Technological Research Council of Turkey (TÜBİTAK). Also, we would like to acknowledge for the financial support provided by Karadeniz Technical University Scientific Research Project Department.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The study was financially supported by Karadeniz Technical University Scientific Research Projects Unit under the grant “THD-2018-7353”.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2020-0305).

Received: 2020-12-25
Accepted: 2021-03-13
Published Online: 2021-04-07
Published in Print: 2021-09-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2020-0305
Keywords for this article
anticancer activity; antioxidant; Barbarea; Brassicaceae; gluconasturtiin choline salt

Articles in the same Issue

  1. Frontmatter
  2. Review Article
  3. A review on chemistry, source and therapeutic potential of lambertianic acid
  4. Research Articles
  5. Neuroprotective constituents of Actaea acuminata (Wall. ex Royle) H. Hara roots
  6. Receptor-mediated biological effects of extracts obtained from three Asplenium species
  7. Evaluation of biological activities of Barbarea integrifolia and isolation of a new glucosinolate derivated compound
  8. Comparative spasmolytic effect between Cinnamomum tamala and Cinnamomum verum leaf essential oils and eugenol through in vitro and in silico approaches
  9. Simple mathematical models for controlling COVID-19 transmission through social distancing and community awareness
  10. Benzylisoquinoline alkaloids from Nelumbo nucifera Gaertn. petals with antiausterity activities against the HeLa human cervical cancer cell line
  11. Cardioprotective effects of omega 3 fatty acids from fish oil and it enhances autoimmunity in porcine cardiac myosin-induced myocarditis in the rat model
  12. New indane derivatives containing 2-hydrazinothiazole as potential acetylcholinesterase and monoamine oxidase-B inhibitors
  13. Rapid communication
  14. Seroprevalence of SARS-CoV-2 in Pakistan: an update on epidemiological trends
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