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Distillation time effecting on the composition of Origanum floribundum essential oils and their antioxidant and antimicrobial activities

  • Lamia Kerbouche EMAIL logo , Mohamed Hazzit , Aoumeur Baaliouamer and Soraya Akretche
Published/Copyright: October 1, 2020
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Zeitschrift für Naturforschung C
From the journal Zeitschrift für Naturforschung C Volume 76 Issue 3-4

Abstract

The essential oils (EOs) of Origanum floribundum Munby, an aromatic and medicinal plant endemic in Algeria, were extracted by different hydrodistillation times (30 min, 1, 2 and 3 h) and analyzed by GC and GC–MS. The chromatographic analysis showed that thymol (32.7–45.0%), p-cymene (16.8–23.1%) and γ-terpinene (21.6–28.7%) were the most prominent components of the oils. The antioxidant ability was measured using the reductive potential, thiobarbituric acid reactive substances (TBARS) assay and the inhibition of free radicals DPPH● and ABTS●+. Antibacterial activity was assessed by the disc diffusion method against three bacteria (Escherichia coli, Staphylococcus aureus and Bacillus subtilis) and one fungus (Candida albicans). Minimal inhibitory concentrations (MICs) were determined using a microdilution method. Thymol is one of the compounds of EOs, which are widely reported as very biologically active. Although the oil isolated for 30 min was the less-thymol rich, it was the most active with all the antioxidant tests. In the most cases, the antimicrobial activity showed the best results with oils isolated for 30 min and 3 h (MIC = 0.25–1.75 μL/mL). These results suggest that it might be possible to isolate the EO from this plant for a minimum distillation time to obtain oil that can give maximum biological activities.

Keywords: antimicrobial activity; antioxidant activity; essential oil; Origanum floribundum; time of extraction
Corresponding author: Lamia Kerbouche, Laboratory of Industrial Process Engineering Sciences, Faculty of Mechanical and Process Engineering, University of Sciences and Technology Houari Boumediene (USTHB), Bab Ezzouar, Algiers, Algeria, E-mail:

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Supplementary material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2020-0102).

Received: 2020-05-12
Accepted: 2020-09-09
Published Online: 2020-10-01
Published in Print: 2021-03-26

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/znc-2020-0102
Keywords for this article
antimicrobial activity; antioxidant activity; essential oil; Origanum floribundum; time of extraction

Articles in the same Issue

  1. Frontmatter
  2. Review Article
  3. A systematic review of botany, phytochemicals and pharmacological properties of “Hoja santa” (Piper auritum Kunth)
  4. Research Articles
  5. Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
  6. Potential insecticidal activity of aminonaphthoquinone Mannich bases derived from lawsone and their copper (II) complex derivatives
  7. Design and synthesis of novel n-butyphthalide derivatives as promising botanical fungicides
  8. Cotinus coggygria Scop. induces cell cycle arrest, apoptosis, genotoxic effects, thermodynamic and epigenetic events in MCF7 breast cancer cells
  9. Elaeocarpus floribundus Bl. seeds as a new source of bioactive compounds with promising antioxidant and antimicrobial properties
  10. Structural elucidation and neuroprotective activities of lignans from the flower buds of Magnolia biondii Pamp.
  11. Distillation time effecting on the composition of Origanum floribundum essential oils and their antioxidant and antimicrobial activities
  12. Root essential oil of Chrysopogon zizanioides relaxes rat isolated thoracic aorta – an ex vivo approach
  13. Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor
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