Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
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Guangren Xu
Abstract
Chikusetsusaponin IVa (CHS-IVa), a saponin from herb Panacis japonicas, possesses extensive biological activities. However, the roles and underlying mechanisms of CHS-IVa on inflammation have not been fully clarified in the setting of murine macrophages. In this study, we found that CHS-IVa effectively reduced the expression of inflammatory mediators, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), interleukin-1β (IL-1β), cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. Meanwhile, CHS-IVa could also evidently bate the contents of nitric oxide (NO) and prostaglandin E2 (PGE2) in cell culture supernatants. Furthermore, the anti-inflammatory activity of CHS-IVa may be via diminishing the phosphorylation of extracellular regulated protein kinases (ERK), p38, and c-Jun N-terminal kinase (JNK). Collectively, these findings will help to understand of the anti-inflammatory effects and mechanisms of P. japonicas deeply, and suggest a validated therapeutic use as an anti-inflammatory medication.
Funding source: Hunan Provincial Innovation Foundation for Postgraduate
Award Identifier / Grant number: CX2015B257 and CX2017B373
Funding source: 2011 Collaborative Innovation Center of Hunan Province for livestock safety production
Acknowledgment
This research was supported by Hunan Provincial Innovation Foundation for Postgraduate (CX2015B257 and CX2017B373), and 2011 Collaborative Innovation Center of Hunan Province for livestock safety production. In addition, we sincerely thank Dr. Wei Wang and Dr. Aibing Wang for providing the experiment material chikusetsusaponin IVa.
Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: Hunan Provincial Innovation Foundation for Postgraduate (CX2015B257 and CX2017B373), and 2011 Collaborative Innovation Center of Hunan Province for livestock safety production.
Conflict of interest statement: The authors declare that there is no conflict of interests.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review Article
- A systematic review of botany, phytochemicals and pharmacological properties of “Hoja santa” (Piper auritum Kunth)
- Research Articles
- Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
- Potential insecticidal activity of aminonaphthoquinone Mannich bases derived from lawsone and their copper (II) complex derivatives
- Design and synthesis of novel n-butyphthalide derivatives as promising botanical fungicides
- Cotinus coggygria Scop. induces cell cycle arrest, apoptosis, genotoxic effects, thermodynamic and epigenetic events in MCF7 breast cancer cells
- Elaeocarpus floribundus Bl. seeds as a new source of bioactive compounds with promising antioxidant and antimicrobial properties
- Structural elucidation and neuroprotective activities of lignans from the flower buds of Magnolia biondii Pamp.
- Distillation time effecting on the composition of Origanum floribundum essential oils and their antioxidant and antimicrobial activities
- Root essential oil of Chrysopogon zizanioides relaxes rat isolated thoracic aorta – an ex vivo approach
- Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor
Articles in the same Issue
- Frontmatter
- Review Article
- A systematic review of botany, phytochemicals and pharmacological properties of “Hoja santa” (Piper auritum Kunth)
- Research Articles
- Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
- Potential insecticidal activity of aminonaphthoquinone Mannich bases derived from lawsone and their copper (II) complex derivatives
- Design and synthesis of novel n-butyphthalide derivatives as promising botanical fungicides
- Cotinus coggygria Scop. induces cell cycle arrest, apoptosis, genotoxic effects, thermodynamic and epigenetic events in MCF7 breast cancer cells
- Elaeocarpus floribundus Bl. seeds as a new source of bioactive compounds with promising antioxidant and antimicrobial properties
- Structural elucidation and neuroprotective activities of lignans from the flower buds of Magnolia biondii Pamp.
- Distillation time effecting on the composition of Origanum floribundum essential oils and their antioxidant and antimicrobial activities
- Root essential oil of Chrysopogon zizanioides relaxes rat isolated thoracic aorta – an ex vivo approach
- Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor
