Absolute configuration of tetrandrine and isotetrandrine influences their anti-proliferation effects in human T cells via different regulation of NF-κB
-
Wencheng Xu
,
Junichi Kusano
,
Haruki Yamada
Abstract
Natural compound tetrandrine was reported to inhibit the proliferation of T cells by inhibiting activation of NF-κB. Chemically, isotetrandrine differs from tetrandrine only in the stereochemistry at the chiral centers. The present study aimed to compare their anti-proliferation effects on human T cells with a focus on NF-κB. The IC50 values of tetrandrine against MOLT-4 cells, MOLT-4/DNR cells, and concanavalin A-activated peripheral blood mononuclear cells of healthy subjects and dialysis patients were 4.43 ± 0.22, 3.62 ± 0.22, 1.91 ± 0.22 and 3.03 ± 0.28 μM, respectively. Whereas, the IC50 values of isotetrandrine against the above immune cells were 2.19 ± 0.27, 2.28 ± 0.33, 1.29 ± 0.14 and 1.55 ± 0.26 μM, respectively. The inhibitory effect of isotetrandrine against the proliferation of T cells was stronger than that of tetrandrine significantly (p < 0.05). Molecular mechanism investigation showed that 10 μM of isotetrandrine largely decreased the expression of p-NF-κB and NF-κB in both MOLT-4 and MOLT-4/DNR T cells (p < 0.05), whereas 10 μM of tetrandrine slightly inhibited the phosphorylation of p-NF-κB with little influence on the expression of NF-κB. Taken together, absolute configurations of tetrandrine and isotetrandrine are suggested to influence on their anti-proliferation effects in human T cells via different regulation of NF-κB.
Funding source: State Scholarship Fund of China Scholarship Council
Award Identifier / Grant number: 201808420024
Funding source: Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan
Award Identifier / Grant number: 15K08081
Funding source: Japan China Sasakawa Medical Fellowship
Award Identifier / Grant number: 2017816
Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by the Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan under Grant (15K08081); Japan China Sasakawa Medical Fellowship under Grant (2017816) and State Scholarship Fund of China Scholarship Council under Grant (201808420024).
Competing interests: The authors declare no conflicts of interest regarding this article.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review Article
- The fate of chlorophyll in phytophagous insects goes beyond nutrition
- Research Articles
- Eleven isoquinoline alkaloids on inhibiting tissue factor activity: structure-activity relationships and molecular docking
- Absolute configuration of tetrandrine and isotetrandrine influences their anti-proliferation effects in human T cells via different regulation of NF-κB
- Safflower injection inhibits pulmonary arterial remodeling in a monocrotaline-induced pulmonary arterial hypertension rat model
- Gas chromatography coupled to mass spectrometry (GC-MS) characterization and evaluation of antibacterial bioactivities of the essential oils from Piper arboreum Aubl., Piper aduncum L. e Piper gaudichaudianum Kunth
- Isolation of secondary metabolites from the Iranian medicinal plant Eremurus persicus
- Decumbic anhydride from the stem barks of Swintonia floribunda (Anacardiaceae)
- Phytochemical characterization of different yarrow species (Achillea sp.) and investigations into their antimicrobial activity
- Monitoring of changes in 5-n-alkylresorcinols during wheat seedling development
- Coumaronochromone as antibacterial and carbonic anhydrase inhibitors from Aerva persica (Burm.f.) Merr.: experimental and first-principles approaches
- Ethyl acetate extract from Cistus x incanus L. leaves enriched in myricetin and quercetin derivatives, inhibits inflammatory mediators and activates Nrf2/HO-1 pathway in LPS-stimulated RAW 264.7 macrophages
- Rapid Communication
- A new xanthone dimer and cytotoxicity from the stem bark of Calophyllum canum
Articles in the same Issue
- Frontmatter
- Review Article
- The fate of chlorophyll in phytophagous insects goes beyond nutrition
- Research Articles
- Eleven isoquinoline alkaloids on inhibiting tissue factor activity: structure-activity relationships and molecular docking
- Absolute configuration of tetrandrine and isotetrandrine influences their anti-proliferation effects in human T cells via different regulation of NF-κB
- Safflower injection inhibits pulmonary arterial remodeling in a monocrotaline-induced pulmonary arterial hypertension rat model
- Gas chromatography coupled to mass spectrometry (GC-MS) characterization and evaluation of antibacterial bioactivities of the essential oils from Piper arboreum Aubl., Piper aduncum L. e Piper gaudichaudianum Kunth
- Isolation of secondary metabolites from the Iranian medicinal plant Eremurus persicus
- Decumbic anhydride from the stem barks of Swintonia floribunda (Anacardiaceae)
- Phytochemical characterization of different yarrow species (Achillea sp.) and investigations into their antimicrobial activity
- Monitoring of changes in 5-n-alkylresorcinols during wheat seedling development
- Coumaronochromone as antibacterial and carbonic anhydrase inhibitors from Aerva persica (Burm.f.) Merr.: experimental and first-principles approaches
- Ethyl acetate extract from Cistus x incanus L. leaves enriched in myricetin and quercetin derivatives, inhibits inflammatory mediators and activates Nrf2/HO-1 pathway in LPS-stimulated RAW 264.7 macrophages
- Rapid Communication
- A new xanthone dimer and cytotoxicity from the stem bark of Calophyllum canum
