Synthetic cell-permeable caveolin-1 scaffolding domain peptide activates phagocytosis of Escherichia coli by regulating Rab5 activity
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Makoto Hagiwara
Abstract
Caveolae are defined as 50–100 nm wide pits in the plasma membrane containing oligomeric caveolin proteins. They have been implicated in endocytosis (including phagocytosis), transcytosis, calcium signalling, and numerous other signal transduction events. Caveolin-1, a major structural component of caveolae, enhances Rab5 activity. In this study, we examined the effect of a synthetic cell-permeable peptide of the caveolin-1 scaffolding domain (CSD) on phagocytosis. Treatment with the CSD peptide increased Rab5 activity, Rab5-early endosome antigen 1 (EEA1) interaction, and phagocytosis of Escherichia coli. The results suggest that the synthetic cell-permeable CSD peptide is an activator of phagocytosis.
Acknowledgements
The authors thank Dr. G. Li (University of Oklahoma Health Science Center) for providing the GST-R5BD vector and Dr. Y. Yamamoto (Tokyo University of Agriculture) for providing the GFP-Rab5 vector.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Historical Research Article
- A tutorial translation of the description of the historically first polymer drug conjugate and its in vivo evaluation
- Research Articles
- The expression and purification of LpxA of Chlamydia trachomatis and preparation of its polyclonal antibody
- Natural antioxidants in emulsions O/W
- Bioactivity-guided isolation of aurone derivatives with hepatoprotective activities from the fruits of Cucumis bisexualis
- Synthetic cell-permeable caveolin-1 scaffolding domain peptide activates phagocytosis of Escherichia coli by regulating Rab5 activity
- Mammalian exocrine secretions XIX. Chemical characterization of the interdigital secretion of the Black Wildebeest, Connochaetes gnou
- Synthesis, investigation of biological effects and in silico studies of new benzimidazole derivatives as aromatase inhibitors
- Review Article
- Coronaviruses widespread on nonliving surfaces: important questions and promising answers
- Rapid Communication
- Synthesis of sandwich type acyclic tetra-nuclear silver(I)-N-heterocyclic carbene complexes for wound healing applications
Artikel in diesem Heft
- Frontmatter
- Historical Research Article
- A tutorial translation of the description of the historically first polymer drug conjugate and its in vivo evaluation
- Research Articles
- The expression and purification of LpxA of Chlamydia trachomatis and preparation of its polyclonal antibody
- Natural antioxidants in emulsions O/W
- Bioactivity-guided isolation of aurone derivatives with hepatoprotective activities from the fruits of Cucumis bisexualis
- Synthetic cell-permeable caveolin-1 scaffolding domain peptide activates phagocytosis of Escherichia coli by regulating Rab5 activity
- Mammalian exocrine secretions XIX. Chemical characterization of the interdigital secretion of the Black Wildebeest, Connochaetes gnou
- Synthesis, investigation of biological effects and in silico studies of new benzimidazole derivatives as aromatase inhibitors
- Review Article
- Coronaviruses widespread on nonliving surfaces: important questions and promising answers
- Rapid Communication
- Synthesis of sandwich type acyclic tetra-nuclear silver(I)-N-heterocyclic carbene complexes for wound healing applications
