Apoptosis induction by α-mangostin-loaded nanoparticles in human cervical carcinoma cells
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Minh H. Nguyen
Abstract
The compound α-mangostin (AMG) extracted from Garcinia mangostana L. has potent anticancer properties but its clinical application is limited because of its poor solubility. In this study, AMG-loaded nanoparticles (NMG) were synthesized using a new formula and their apoptosis activity against human cervical carcinoma (HeLa) cells was investigated in comparison with organic solvent-soluble AMG in free form. The NMG was successfully synthesized with a particle size of <50 nm, polydispersity index <0.3, and zeta potential of −35.2 mV. At a concentration of 10 μg/mL, AMG reduced cell survival by 60%, whereas NMG treatment resulted in >90% cell death (p < 0.05). The AMG- or NMG-treated cells also showed changes in the size and shape and exhibited enhanced intensity of blue-stained nuclei, as well as decreased cell density, especially in NMG-treated cells. After 24 h of incubation with AMG or NMG, the cells went through late apoptosis at a rate of approximately 34% in 20 μg/mL AMG treatment and 27% in 10 μg/mL NMG treatment (p < 0.05). Thus, HeLa cells underwent more pronounced cell death through apoptosis induction caused by the NMG treatment compared to that caused by AMG. Clearly, the new NMG improved AMG bioavailability while maintaining the desired activity.
Acknowledgments
This work was supported by NAFOSTED grant 106-NN.02-2016.19. The authors thank Dr. Lam L. Nguyen for his help in FTIR analysis.
Conflict of interest statement: We declare that we have no conflict of interest.
Authors’ contributions: Phuong T.M. Nguyen designed the project, performed the experiments, analyzed data, and wrote the manuscript. Minh T.H. Nguyen and Dat T. Nguyen performed the experiments and analyzed data. All authors have read and approved the final manuscript.
Data availability statement: The supplementary data used to support the findings of this study are available from the corresponding author upon request.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2020-0001).
©2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Acute toxicity of Potentilla anserina L. extract in mice
- Selective cytotoxic activity of isolated compounds from Globimetula dinklagei and Phragmanthera capitata (Loranthaceae)
- Apoptosis induction by α-mangostin-loaded nanoparticles in human cervical carcinoma cells
- Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and α-glycosidase inhibitors
- Diterpenoid and phenolic contents of Sideritis hololeuca Boiss & Heldr. Apud Bentham with antioxidant and anticholinesterase activity
- Rapid communication
- Chemical composition and biological activities of Dipterocarpus cornutus Dyer essential oil
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Acute toxicity of Potentilla anserina L. extract in mice
- Selective cytotoxic activity of isolated compounds from Globimetula dinklagei and Phragmanthera capitata (Loranthaceae)
- Apoptosis induction by α-mangostin-loaded nanoparticles in human cervical carcinoma cells
- Secondary metabolites of Helichrysum plicatum DC. subsp. plicatum flowers as strong carbonic anhydrase, cholinesterase and α-glycosidase inhibitors
- Diterpenoid and phenolic contents of Sideritis hololeuca Boiss & Heldr. Apud Bentham with antioxidant and anticholinesterase activity
- Rapid communication
- Chemical composition and biological activities of Dipterocarpus cornutus Dyer essential oil
