Anti-acetylcholinesterase activity of essential oils and their major constituents from four Ocimum species
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Mohamed A. Farag
,
Maha M. Salama
Abstract
Ocimum is a genus of considerable importance in traditional medicine worldwide. The goal of this study was to examine the anti-acetylcholinesterase activity of Ocimum essential oils and to correlate the activity with their chemical profiles using a metabolome based GC-MS approach coupled to chemometrics. Further, molecular docking was adopted to rationalize the activity of some essential oil isolates. Essential oil prepared from the four species O. basilicum, O. africanum, O. americanum, and O. minimum exhibited significant anti-acetylcholinesterase activity with (IC50 0.22, 0.175, 0.57 and 0.152 mg/mL, respectively) comparable to that of physostigmine (IC50 0.27 mg/mL). The phenylpropanoids (i.e. estragole) constituted the most dominant chemical group in O. basilicum (sweet basil) and O. minimum, whereas camphor (a ketone) was the most abundant in O. africanum and O. americanum. Supervised and unsupervised multivariate data analyses clearly separated O. africanum and O. americanum from other accessions, with estragole, camphor and, to less extent, β-linalool contributing to species segregation. Estragole was found the most active AchE inhibitor (IC50 0.337 µM) followed by cineole (IC50 2.27 µM), camphor (IC50 21.43 µM) and eugenol (IC50 40.32 µM). Molecular docking revealed that these compounds bind to key amino acids in the catalytic domain of AchE, similar to standard drugs.
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Supplemental Material:
The online version of this article (DOI: 10.1515/znc-2016-0030) offers supplementary material, available to authorized users.
Article note:
This work was presented at the 3rd Conference on Traditional and Alternative Medicine in Birmingham, UK, in 2015.
©2016 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Seasonal variation of gastroprotective terpenoids in Maytenus robusta (Celastraceae) quantified by gas chromatography-flame ionization detection (GC-FID)
- Plicosepalin A, a new antioxidant catechin–gallic acid derivative of inositol from the mistletoe Plicosepalus curviflorus
- New lupan-type triterpenoids
- Penicilloitins A and B, new antimicrobial fatty acid esters from a marine endophytic Penicillium species
- Anti-acetylcholinesterase activity of essential oils and their major constituents from four Ocimum species
- The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils
- Biological evaluation and docking studies of some benzoxazole derivatives as inhibitors of acetylcholinesterase and butyrylcholinesterase
- LC/ESI-MS/MS profiling of Ulmus parvifolia extracts and evaluation of its anti-inflammatory, cytotoxic, and antioxidant activities
- Antiproliferative effect of synthetic cyclic imides (methylphtalimides, carboxylic acid phtalimides and itaconimides) against human cancer cell lines
- Anti-inflammatory activity of highly oxygenated terpenoids from Achillea biebersteinii Afan
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Biosciences – Zeitschrift für Naturforschung C, volume 71 (2016)
Artikel in diesem Heft
- Frontmatter
- Research Articles
- Seasonal variation of gastroprotective terpenoids in Maytenus robusta (Celastraceae) quantified by gas chromatography-flame ionization detection (GC-FID)
- Plicosepalin A, a new antioxidant catechin–gallic acid derivative of inositol from the mistletoe Plicosepalus curviflorus
- New lupan-type triterpenoids
- Penicilloitins A and B, new antimicrobial fatty acid esters from a marine endophytic Penicillium species
- Anti-acetylcholinesterase activity of essential oils and their major constituents from four Ocimum species
- The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils
- Biological evaluation and docking studies of some benzoxazole derivatives as inhibitors of acetylcholinesterase and butyrylcholinesterase
- LC/ESI-MS/MS profiling of Ulmus parvifolia extracts and evaluation of its anti-inflammatory, cytotoxic, and antioxidant activities
- Antiproliferative effect of synthetic cyclic imides (methylphtalimides, carboxylic acid phtalimides and itaconimides) against human cancer cell lines
- Anti-inflammatory activity of highly oxygenated terpenoids from Achillea biebersteinii Afan
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Biosciences – Zeitschrift für Naturforschung C, volume 71 (2016)
