125I–Amoxicillin preparation as a guide tracer for inflammation detection

Marwa S. El Refaye; Dina M. El-Sharawy; Heba Hussien

doi:10.1515/ract-2021-1077

Article

¹²⁵I–Amoxicillin preparation as a guide tracer for inflammation detection

Marwa S. El Refaye , Dina M. El-Sharawy and Heba Hussien

Published/Copyright: August 23, 2021

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From the journal Radiochimica Acta Volume 109 Issue 10

Abstract

The objective of this study is to label Amoxicillin with radioactive iodine (¹²⁵I-AC) via direct electrophilic substitution to act as a promising tracer for inflammation imaging. The highest labeling yield of 80% was achieved after studying all the parameters affecting the labeling reaction using Iodogen (IG) as an oxidizing agent. Molecular Modeling Structure was done using MOE program to predict the suitable ¹²⁵I position. The product structure was established by a cold iodination reaction using Iodine-127. Biological evaluation of (¹²⁵I-AC) was carried out using groups of inflamed mice with different exogenous agents such as E.Coli and Turpentine oil. The (¹²⁵I-AC) shows an in vitro stability of about 97% after 24 h.While doing in vivo studies over 4 h, the tracer stability of 99% was observed.

Keywords: 125I; amoxicillin; cold iodination reaction; iodogen; MOE; radiolabeling yield

Corresponding author: Marwa S. El Refaye, Radioactive Isotopes and Generators Department, Hot Laboratory Center, Egyptian Atomic Energy Authority (EAEA), P.O. Box 13759, Cairo, Egypt; and Cyclotron Project, Nuclear Research Centre, Egyptian Atomic Energy Authority (EAEA), P.O. Box 13759, Cairo, Egypt, E-mail: mirouchoucri@hotmail.com

Acknowledgement

The authors would like to acknowledge Dr Ahmed Rashad for his valuable comments and support to complete this manuscript.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2021-07-13

Accepted: 2021-08-06

Published Online: 2021-08-23

Published in Print: 2021-10-26

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Articles in the same Issue

https://doi.org/10.1515/ract-2021-1077

Keywords for this article

125I; amoxicillin; cold iodination reaction; iodogen; MOE; radiolabeling yield