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Fabrication of avian eggshell membrane derived dispersed collagen hydrogels for potential bone regeneration

  • Aakriti Aggarwal and Mahesh Kumar Sah EMAIL logo
Published/Copyright: August 8, 2023
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Journal of Polymer Engineering
From the journal Journal of Polymer Engineering Volume 43 Issue 8

Abstract

Tissue engineering is emerging as an effective and alternate strategy for healing the impaired bones. This study reports the development of potential and novel bone tissue regenerating matrices from the avian eggshell membrane derived collagen dispersed in poly-(vinyl alcohol) (PVA). The concentration of the aqueous collagen dispersions within the PVA solution were varied from 0.5 % to 2 % (w/V); and the sols were subjected to varying freeze-thaw cycles to achieve gelation; leading to the fabrication of hydrogel matrices. The developed hydrogels were investigated for their physico-chemical characteristics through the ATR-FTIR, XRD, FESEM and biocompatibility measurements. The ATR-FTIR results showed the presence of amide A, amide I and hydroxyl groups; which were further reinstated by the XRD studies. The morphological and topological analysis of the different hydrogel groups was made through FESEM. It comprised of the measurements of pore dimensions ranging (3.98 ± 1.84 µm to 9.24 ± 5.55 µm), percentage porosity (47 %–97 %), and average surface roughness (21–39 µm); thus, indicating them to be analogous support systems for bone tissue regeneration. Further, L929 mouse fibroblasts grown over these support systems showed excellent cell viability, thus hinting towards its competitive features and application towards remedial bone regeneration.

Keywords: biocompatibility; bone tissue engineering; eggshell membrane collagen; hydrogel matrices; sol-gel transition
Corresponding author: Mahesh Kumar Sah, Department of Biotechnology, Dr. B. R. Ambedkar National Institute of Technology, Jalandhar, Punjab 144011, India, E-mail:

Acknowledgments

The authors are thankful to Prof. A. Chatterjee (Dept. of Textile Technology, NIT Jalandhar, India) for ATR-FTIR measurements and Liveon Biolabs Private Limited, Karnataka, India for in vitro cell studies.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The authors acknowledge the support from MHRD (Govt. of India) to the first author, Ms. Aakriti Aggarwal, for completion of this research work.

  3. Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.

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Received: 2023-03-26
Accepted: 2023-06-26
Published Online: 2023-08-08
Published in Print: 2023-09-26

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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  2. Material Properties
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https://doi.org/10.1515/polyeng-2023-0071
Keywords for this article
biocompatibility; bone tissue engineering; eggshell membrane collagen; hydrogel matrices; sol-gel transition

Articles in the same Issue

  1. Frontmatter
  2. Material Properties
  3. A brief review on polymer nanocomposites: current trends and prospects
  4. Recent development in the formation and surface modification of cellulose-bead nanocomposites as adsorbents for water purification: a comprehensive review
  5. CdSe nanodots to nanorods in PVA films: effect of shape transition and loading on the opto-mechanical and biodegradation properties
  6. Kinetic and thermodynamic studies of H2S adsorption by lignin-based composite membranes
  7. Preparation and Assembly
  8. Fabrication of avian eggshell membrane derived dispersed collagen hydrogels for potential bone regeneration
  9. Antimicrobially effective protein-loaded metal chelated chitosan composite
  10. Engineering and Processing
  11. Highly thermally conductive polyamide 6 composites with favorable mechanical properties, processability and low water absorption using a hybrid filling of short carbon fiber, flake graphite and expanded graphite
  12. Tribological behavior of ultra-high molecular weight polyethylene (UHMWPE) for acetabular replacement under frictional heat based on molecular dynamics
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