Fabrication and characterization of three-dimensional polycaprolactone/sodium alginate and egg whites and eggshells hybrid scaffold in bone tissue engineering
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Hessam Rezaei
,
Mostafa Shahrezaee
,
Marziyeh Jalali Monfared
Abstract
The aim of this study was to fabricate three-dimensional bone scaffolds using polycaprolactone and egg shell powder. The scaffolds were coated with sodium alginate/egg white. SEM was used to investigate egg shell particles on the surface of each string of scaffolds. The presence of calcium carbonate in the scaffold structure was confirmed by microstructural analyses employing XRD. Egg shell-related functional groups were discovered using FTIR investigations. Bone cells were used to conduct biocompatibility tests on scaffolds (MG-63). Finally, scaffolds with the highest proportion of egg whites and eggshells have the best cell survival rate. It may be concluded that the PCL/7% Esh/15% Ew scaffold is a good option for application in bone tissue engineering.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: None declared.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
References
1. Wang, Q., Yan, J., Yang, J., Li, B. Nanomaterials promise better bone repair. Mater. Today 2016, 19, 451–463; https://doi.org/10.1016/j.mattod.2015.12.003.Search in Google Scholar
2. Trakoolwannachai, V., Kheolamai, P., Ummartyotin, S. Characterization of hydroxyapatite from eggshell waste and polycaprolactone (PCL) composite for scaffold material. Compos. B Eng. 2019, 173, 106974; https://doi.org/10.1016/j.compositesb.2019.106974.Search in Google Scholar
3. Basirun, W. J., Nasiri-Tabrizi, B., Baradaran, S. Overview of hydroxyapatite–graphene nanoplatelets composite as bone graft substitute: mechanical behavior and in-vitro biofunctionality. Crit. Rev. Solid State Mater. Sci. 2018, 43, 177–212; https://doi.org/10.1080/10408436.2017.1333951.Search in Google Scholar
4. Alves Cardoso, D., Jansen, J. A., Leeuwenburgh, S. C. G. Synthesis and application of nanostructured calcium phosphate ceramics for bone regeneration. J. Biomed. Mater. Res. Part B Appl. Biomater. 2012, 100, 2316–2326; https://doi.org/10.1002/jbm.b.32794.Search in Google Scholar PubMed
5. Oryan, A., Alidadi, S., Moshiri, A., Maffulli, N. Bone regenerative medicine: classic options, novel strategies, and future directions. J. Orthop. Surg. Res. 2014, 9, 1–27; https://doi.org/10.1186/1749-799X-9-18.Search in Google Scholar PubMed PubMed Central
6. Chandramohan, Y., Jaganathan, K., Sivanesan, S., Koka, P., Madom Seetaraman Amritha, T., Vimalraj, S., Dhanasekaran, A. Assessment of human ovarian follicular fluid derived mesenchymal stem cells in chitosan/PCL/Zn scaffold for bone tissue regeneration. Life Sci. 2021, 264, 118502; https://doi.org/10.1016/j.lfs.2020.118502.Search in Google Scholar PubMed
7. Mohseni, M., Dezdouli, Z. Flake-like glass nanoparticles reinforced natural polymeric scaffold as a promising substrate for bone regeneration. J. Polym. Res. 2020, 27, 1–10; https://doi.org/10.1007/s10965-020-02230-1.Search in Google Scholar
8. Huang, Y. z., Ji, Y. R., Kang, Z. W., Li, F. Integrating eggshell-derived CaCO3/MgO nanocomposites and chitosan into a biomimetic scaffold for bone regeneration. Chem. Eng. J. 2020, 395, 125098; https://doi.org/10.1016/j.cej.2020.125098.Search in Google Scholar
9. Wang, N., Dheen, T., Ying His Fuh, J., Senthil Kumar, A. A review of multi-functional ceramic nanoparticles in 3D printed bone tissue engineering. Bioprinting 2021, 23, e00146; https://doi.org/10.1016/j.bprint.2021.e00146.Search in Google Scholar
10. Iglesias-Mejuto, A., García-González, C. A. 3D-printed alginate-hydroxyapatite aerogel scaffolds for bone tissue engineering. Mater. Sci. Eng. C. 2021, 131, 112525; https://doi.org/10.1016/j.msec.2021.112525.Search in Google Scholar PubMed
11. Delkash, Y., Gouin, M., Rimbealt, T., Mohabatpour, F., Papagerakis, P., Maw, S., Chen, X. Bioprinting and in vitro characterization of an eggwhite-based cell-laden patch for endothelialized tissue engineering applications. J. Funct. Biomater. 2021, 12, 45; https://doi.org/10.3390/jfb12030045.Search in Google Scholar PubMed PubMed Central
12. Geng, F., Huang, X., Ma, M. Hen egg white ovomacroglobulin promotes fibroblast migration via mediating cell adhesion and cytoskeleton. J. Sci. Food Agric. 2016, 96, 3188–3194; https://doi.org/10.1002/jsfa.7498.Search in Google Scholar PubMed
13. Waku, T., Kanamaru, K., Hiroyama, Y., Sasaki, R., Morimoto, N., Tanaka, N. Preparation of nanoparticles composed of egg white protein and their application for cell adhesion control. Kobunshi Ronbunshu 2018, 75, 61–68; https://doi.org/10.1295/koron.2017-0070.Search in Google Scholar
14. Kaipparettu, B. A., Kuiatse, I., Tak-Yee Chan, B., Kiaparettu, M. B., Lee, A. V., Oesterreich, S. Novel egg white–based 3-D cell culture system. Biotechniques 2008, 45, 165–171; https://doi.org/10.2144/000112883.Search in Google Scholar PubMed
15. Zanelato, C. B., Pires, A. F., Silva, S. N., Galdino, A. G. S. Development of biphasic bone cement obtained from chicken eggshell. J. Mater. Res. Technol. 2020, 9, 7297–7304; https://doi.org/10.1016/j.jmrt.2020.05.053.Search in Google Scholar
16. Arslan, Y. E., Arsalan, T. S., Derkus, B., Emregul, E., Emregul, C. K. Fabrication of human hair keratin/jellyfish collagen/eggshell-derived hydroxyapatite osteoinductive biocomposite scaffolds for bone tissue engineering: from waste to regenerative medicine products. Colloids Surf., B 2017, 154, 160–170; https://doi.org/10.1016/j.colsurfb.2017.03.034.Search in Google Scholar PubMed
17. Nga, N. K., Chau, N. T. T., Viet, P. H. Facile synthesis of hydroxyapatite nanoparticles mimicking biological apatite from eggshells for bone-tissue engineering. Colloids Surf., B 2018, 172, 769–778; https://doi.org/10.1016/j.colsurfb.2018.09.039.Search in Google Scholar PubMed
18. Unagolla, J. M., Jayasuriya, A. C. Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds. Mater. Sci. Eng. C. 2019, 102, 1–11; https://doi.org/10.1016/j.msec.2019.04.026.Search in Google Scholar PubMed PubMed Central
19. Xu, X., Yan, H., Deng, Y., Du, J. Recent advances in design of functional biocompatible hydrogels for bone tissue engineering. Adv. Funct. Mater. 2021, 31, 2009432.10.1002/adfm.202009432Search in Google Scholar
20. Xue, X., Hu, Y., Wang, S., Chen, X., Jiang, Y., Su, J. Fabrication of physical and chemical crosslinked hydrogels for bone tissue engineering. Bioact. Mater. 2022, 12, 327–339; https://doi.org/10.1016/j.bioactmat.2021.10.029.Search in Google Scholar PubMed PubMed Central
21. Zhou, R., Guo, Q., Xiao, Y., Guo, Q., Huang, Y., Li, C., Luo, X. Endocrine role of bone in the regulation of energy metabolism. Bone Res. 2021, 9, 25; https://doi.org/10.1038/s41413-021-00142-4.Search in Google Scholar PubMed PubMed Central
22. Dong, Q., Zhang, M., Zhou, X., Shao, Y., Li, J., Wang, L., Chu, C., Xue, F., Yao, Q., Bai, J. 3D-printed Mg-incorporated PCL-based scaffolds: a promising approach for bone healing. Mater. Sci. Eng. C. 2021, 129, 112372; https://doi.org/10.1016/j.msec.2021.112372.Search in Google Scholar PubMed
23. Ummartyotin, S., Pisitsak, P., Pechyen, C. Eggshell and bacterial cellulose composite membrane as absorbent material in active packaging. Int. J. Polym. Sci. 2016, 2016, 1047606.10.1155/2016/1047606Search in Google Scholar
24. Naga, M. S., EI Maghreby, H., Sayed, M. Highly porous scaffolds made of nanosized hydroxyapatite powder synthesized from eggshells. J. Ceram. Sci. Technol. 2015, 6, 237–244.Search in Google Scholar
25. Zhang, Y., Li, H., Zhang, Y., Song, F., Cao, X., Lyu, X., Crittenden, J. Statistical optimization and batch studies on adsorption of phosphate using Al-eggshell. Adsorpt. Sci. Technol. 2018, 36, 999–1017; https://doi.org/10.1177/0263617417740790.Search in Google Scholar
26. Balu, R., Kumar, T., Ramalingam, M., Ramakrishna, S. Electrospun polycaprolactone/poly (1, 4-butylene adipate-co-polycaprolactam) blends: potential biodegradable scaffold for bone tissue regeneration. J. Biomater. Tissue Eng. 2011, 1, 30–39; https://doi.org/10.1166/jbt.2011.1004.Search in Google Scholar
27. Xue, X., Zhang, H., Liu, H., Wang, S., Li, J., Zhou, Q., Chen, X., Ren, X., Jing, Y., Deng, Y. Rational design of multifunctional CuS nanoparticle-PEG composite soft hydrogel-coated 3D hard polycaprolactone scaffolds for efficient bone regeneration. Adv. Funct. Mater. 2022, 32, 2202470; https://doi.org/10.1002/adfm.202202470.Search in Google Scholar
28. Zhao, X., Wu, Y., Du, Y., Chen, X., Lei, B., Xue, Y., Ma, P. A highly bioactive and biodegradable poly (glycerol sebacate)–silica glass hybrid elastomer with tailored mechanical properties for bone tissue regeneration. J. Mater. Chem. B 2015, 3, 3222–3233; https://doi.org/10.1039/c4tb01693a.Search in Google Scholar PubMed
29. Sangeetha, R., Madheswari, D., Priya, G. Fabrication of poly (methyl methacrylate)/Ce/Cu substituted apatite/egg white (ovalbumin) biocomposite owning adjustable properties: towards bone tissue rejuvenation. J. Photochem. Photobiol. B Biol. 2018, 187, 162–169; https://doi.org/10.1016/j.jphotobiol.2018.08.015.Search in Google Scholar PubMed
30. Pham, H. M., Zhang, Y., Munguia-Lopez, J. G., Tran, D. S. Egg white alginate as a novel scaffold biomaterial for 3D salivary cell culturing. Biomimetics 2021, 7, 5; https://doi.org/10.3390/biomimetics7010005.Search in Google Scholar PubMed PubMed Central
© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Material Properties
- Structural evolution and barrier properties in biaxially stretched polyethylene terephthalate/hydroxy-terminated polybutadiene films
- Characterizing CaCO3 particle dispersion in blown film
- Tuning rheological performance of silica concentrated shear thickening fluid by using boric acid as additive
- Mechanical and morphological characterization of recycled HD-PE bio-composites based on alfa fibers and natural pozzolan
- Preparation and Assembly
- Boosting the photocatalytic performance of the oligo (phenylene vinylene) moiety by copolymerization for the heterogeneous degradation of indigo carmine dye
- Fabrication and characterization of three-dimensional polycaprolactone/sodium alginate and egg whites and eggshells hybrid scaffold in bone tissue engineering
- In situ prepared composite of polypyrrole and multi-walled carbon nanotubes grafted with sodium polystyrenesulfonate as ammonia gas sensor with wide detection range
- Engineering and Processing
- Properties of compression molded ultra-high molecular weight polyethylene: effects of varying process conditions
- Formulating calcium carbonate masterbatches
- Online detection of residual stress near the gate using cavity pressure for injection molding
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Journal of Polymer Engineering volume 42 (2022)
Articles in the same Issue
- Frontmatter
- Material Properties
- Structural evolution and barrier properties in biaxially stretched polyethylene terephthalate/hydroxy-terminated polybutadiene films
- Characterizing CaCO3 particle dispersion in blown film
- Tuning rheological performance of silica concentrated shear thickening fluid by using boric acid as additive
- Mechanical and morphological characterization of recycled HD-PE bio-composites based on alfa fibers and natural pozzolan
- Preparation and Assembly
- Boosting the photocatalytic performance of the oligo (phenylene vinylene) moiety by copolymerization for the heterogeneous degradation of indigo carmine dye
- Fabrication and characterization of three-dimensional polycaprolactone/sodium alginate and egg whites and eggshells hybrid scaffold in bone tissue engineering
- In situ prepared composite of polypyrrole and multi-walled carbon nanotubes grafted with sodium polystyrenesulfonate as ammonia gas sensor with wide detection range
- Engineering and Processing
- Properties of compression molded ultra-high molecular weight polyethylene: effects of varying process conditions
- Formulating calcium carbonate masterbatches
- Online detection of residual stress near the gate using cavity pressure for injection molding
- Annual Reviewer Acknowledgement
- Reviewer acknowledgement Journal of Polymer Engineering volume 42 (2022)
