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Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization

  • Kadir Erol ORCID logo EMAIL logo , Demet Tatar , Aysel Veyisoğlu and Ali Tokatlı
Published/Copyright: December 16, 2020
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Journal of Polymer Engineering
From the journal Journal of Polymer Engineering Volume 41 Issue 2

Abstract

Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 μg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.

Keywords: activity; adsorption; enzyme immobilization; lysozyme; magnetic microparticle
Corresponding author: Kadir Erol, Department of Medical Services and Techniques, Vocational School of Health Services, Hitit University, Ululavak Street, Çorum19030, Turkey, E-mail:

Acknowledgments

Because of their contributions to the study, we would like to cordially thank Prof. Dr. Dursun Ali Köse (Faculty of Art and Science, Department of Chemistry, Hitit University) and Prof. Dr. Nevzat Şahin (Faculty of Art and Science, Department of Biology, Ondokuz Mayıs University).

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

References

1. Mokadem, Z., Saidi-Besbes, S., Lebaz, N., Elaissari, A. React. Funct. Polym. 2020, 155, 104693; https://doi.org/10.1016/j.reactfunctpolym.2020.104693.Search in Google Scholar

2. Dinali, L. A. F., de Oliveira, H. L., Teixeira, L. S., da Silva, A. T. M., D’Oliveira, K. A., Cuin, A., Borges, K. B. Microchem. J. 2020, 154, 104648; https://doi.org/10.1016/j.microc.2020.104648.Search in Google Scholar

3. Wichaita, W., Polpanich, D., Kaewsaneha, C., Jangpatarapongsa, K., Tangboriboonrat, P. Colloids Surf. B Biointerfaces 2019, 184, 110557; https://doi.org/10.1016/j.colsurfb.2019.110557.Search in Google Scholar PubMed

4. Fang, L., Miao, Y., Wei, D., Zhang, Y., Zhou, Y. Chemosphere 2020, 262, 128032; https://doi.org/10.1016/j.chemosphere.2020.128032.Search in Google Scholar PubMed

5. Tang, F., Ma, N., Tong, L., He, F., Li, L. Langmuir 2011, 28, 883–888; https://doi.org/10.1021/la203704j.Search in Google Scholar PubMed

6. Wang, H., Wang, R., Wang, L., Tian, X. Colloid. Surface. Physicochem. Eng. Aspect. 2011, 384, 624–629; https://doi.org/10.1016/j.colsurfa.2011.05.031.Search in Google Scholar

7. Sacanna, S., Philipse, A. Langmuir 2006, 22, 10209–10216; https://doi.org/10.1021/la0616505.Search in Google Scholar PubMed

8. Li, Y., Ding, M. J., Wang, S., Wang, R. Y., Wu, X. L., Wen, T. T., Yuan, L. H., Dai, P., Lin, Y. H., Zhou, X. M. ACS Appl. Mater. Interfaces 2011, 3, 3308–3315; https://doi.org/10.1021/am2007855.Search in Google Scholar PubMed

9. Kubo, T., Otsuka, K. Trac. Trends Anal. Chem. 2016, 81, 102–109; https://doi.org/10.1016/j.trac.2015.08.008.Search in Google Scholar

10. Sarkar, S., Gulati, K., Mishra, A., Poluri, K. M. Int. J. Biol. Macromol. 2020, 467–482; https://doi.org/10.1016/j.ijbiomac.2020.02.179.Search in Google Scholar PubMed

11. Sharma, M., Jaiswal, N., Kumar, D., Poluri, K. M. Biochem. J. 2019, 476, 613–628; https://doi.org/10.1042/bcj20180703.Search in Google Scholar PubMed

12. Kobayakawa, S., Nakai, Y., Akiyama, M., Komatsu, T. Chem. Eur J. 2017, 23, 5044–5050; https://doi.org/10.1002/chem.201605055.Search in Google Scholar PubMed

13. Alveroglu, E., İlker, N., Shah, M. T., Rajar, K., Gokceoren, A. T., Koc, K. Colloids Surf. B Biointerfaces 2019, 181, 981–988; https://doi.org/10.1016/j.colsurfb.2019.05.062.Search in Google Scholar PubMed

14. Flores-Rojas, G. G., Pino-Ramos, V. H., López-Saucedo, F., Concheiro, A., Alvarez-Lorenzo, C., Bucio, E. Eur. Polym. J. 2017, 95, 27–40; https://doi.org/10.1016/j.eurpolymj.2017.07.040.Search in Google Scholar

15. Kubiak-Ossowska, K., Cwieka, M., Kaczynska, A., Jachimska, B., Mulheran, P. A. Phys. Chem. Chem. Phys. 2015, 17, 24070–24077; https://doi.org/10.1039/c5cp03910j.Search in Google Scholar PubMed

16. Inanan, T., Tüzmen, N., Karipcin, F. Int. J. Biol. Macromol. 2018, 114, 812–820; https://doi.org/10.1016/j.ijbiomac.2018.04.006.Search in Google Scholar PubMed

17. Bayazidi, P., Almasi, H., Asl, A. K. Int. J. Biol. Macromol. 2018, 107, 2544–2551; https://doi.org/10.1016/j.ijbiomac.2017.10.137.Search in Google Scholar PubMed

18. Nartop, D., Yetim, N. K., Özkan, E. H., Sarı, N. J. Mol. Struct. 2020, 1200, 127039; https://doi.org/10.1016/j.molstruc.2019.127039.Search in Google Scholar

19. Li, Y., Zhang, C., Sun, Y. Chin. J. Chem. Eng. 2020, 28, 242–248; https://doi.org/10.1016/j.cjche.2019.03.002.Search in Google Scholar

20. Ward, K., Taylor, A., Mohammed, A., Stuckey, D. C. Adv. Colloid Interface Sci. 2020, 275, 102079; https://doi.org/10.1016/j.cis.2019.102079.Search in Google Scholar PubMed

21. Zhang, H., Li, H., Wang, K., Xia, Q., Zhou, D. Microporous Mesoporous Mater. 2020, 298, 110098; https://doi.org/10.1016/j.micromeso.2020.110098.Search in Google Scholar

22. Erol, B., Erol, K., Gökmeşe, E. Process Biochem. 2019, 83, 104–113; https://doi.org/10.1016/j.procbio.2019.05.009.Search in Google Scholar

23. Erol, K., Cebeci, B. K., Köse, K., Köse, D. A. Int. J. Biol. Macromol. 2019, 123, 738–743; https://doi.org/10.1016/j.ijbiomac.2018.11.121.Search in Google Scholar PubMed

24. Erol, K. J. Turk. Chem. Soc. Sec. A: Chem. 2017, 4, 133–148.10.18596/jotcsa.287321Search in Google Scholar

25. Jesionowski, T., Zdarta, J., Krajewska, B. Adsorption 2014, 20, 801–821; https://doi.org/10.1007/s10450-014-9623-y.Search in Google Scholar

26. Xu, W., Lou, Y., Xu, B., Li, Y., Xiong, Y., Jing, J. Int. J. Biol. Macromol. 2018, 120, 2175–2179; https://doi.org/10.1016/j.ijbiomac.2018.09.041.Search in Google Scholar PubMed

27. Erol, K., Köse, K., Uzun, L., Say, R., Denizli, A. Colloids Surf. B Biointerfaces 2016, 146, 567–576; https://doi.org/10.1016/j.colsurfb.2016.06.060.Search in Google Scholar PubMed

28. Forov, Y., Paulus, M., Dogan, S., Salmen, P., Weis, C., Gahlmann, T., Behrendt, A., Albers, C., Elbers, M., Schnettger, W. Langmuir 2018, 34, 5403–5408; https://doi.org/10.1021/acs.langmuir.8b00280.Search in Google Scholar PubMed

29. Lin, Z. A., Zheng, J. N., Lin, F., Zhang, L., Cai, Z., Chen, G.-N. J. Mater. Chem. 2011, 21, 518–524; https://doi.org/10.1039/c0jm02300k.Search in Google Scholar

30. Orhan, H., Evli, S., Dabanca, M. B., Başbülbül, G., Uygun, M., Uygun, D. A. Mater. Sci. Eng. C 2019, 94, 558–564; https://doi.org/10.1016/j.msec.2018.10.003.Search in Google Scholar PubMed

31. Köse, K. J. Turk. Chem. Soc. Sec. A: Chem. 2016, 3, 185–204; https://doi.org/10.18596/jotcsa.74979.Search in Google Scholar

32. Bilgin, E., Erol, K., Köse, K., Köse, D. A. Environ. Sci. Pollut. Control Ser. 2018, 25, 27614–27627; https://doi.org/10.1007/s11356-018-2784-6.Search in Google Scholar PubMed

33. Jing, M., Song, W., Liu, R. Spectrochim. Acta Mol. Biomol. Spectrosc. 2016, 164, 103–109.10.1016/j.saa.2016.04.008Search in Google Scholar PubMed

34. Ghosh, S., Pandey, N. K., Bhattacharya, S., Roy, A., Nagy, N. V., Dasgupta, S. Int. J. Biol. Macromol. 2015, 76, 1–9; https://doi.org/10.1016/j.ijbiomac.2015.02.014.Search in Google Scholar PubMed

35. Erol, K., Köse, K., Köse, D. A., Sızır, Ü., Tosun Satır, İ., Uzun, L. Desalin. Water Treat. 2016, 57, 9307–9317; https://doi.org/10.1080/19443994.2015.1030708.Search in Google Scholar

36. Köse, K., Erol, K., Emniyet, A. A., Köse, D. A., Avcı, G. A., Uzun, L. Appl. Biochem. Biotechnol. 2015, 177, 1025–1039; https://doi.org/10.1007/s12010-015-1794-9.Search in Google Scholar PubMed

37. Altıntaş, E. B., Denizli, A. Int. J. Biol. Macromol. 2006, 38, 99–106.10.1016/j.ijbiomac.2006.01.011Search in Google Scholar PubMed

38. Lawal, A., Obaleye, J., Adediji, J., Amolegbe, S., Bamigboye, M., Yunus-Issa, M. J. Appl. Sci. Environ. Manag. 2014, 18, 205–208; https://doi.org/10.4314/jasem.v18i2.8.Search in Google Scholar

39. Ji, Y. J. Food Eng. 2020, 285, 110088; https://doi.org/10.1016/j.jfoodeng.2020.110088.Search in Google Scholar

40. Ellis, A. E. Techniques in Fish Immunology; Stolen, J. S., Fletcher, T. C., Anderson, D. P., Roberson, B. S., Mulswink, W. B., Eds., SOS Publications: Fair Haven, New Jersey, USA, 1996; p. 101.Search in Google Scholar

41. Thornsberry, C. Lab. Med. 2016, 14, 549–553.10.1093/labmed/14.9.549Search in Google Scholar

42. Erol, K., Uzunoglu, A., Köse, K., Sarıca, B., Avcı, E., Köse, D. A. J. Chromatogr. B 2018, 1081, 1–7; https://doi.org/10.1016/j.jchromb.2018.02.017.Search in Google Scholar PubMed

43. Erol, K., Köse, K., Güngüneş, H., Köse, D. A. J. Mol. Struct. 2017, 1130, 753–759; https://doi.org/10.1016/j.molstruc.2016.11.004.Search in Google Scholar

44. Elmogy, M., Bassal, T. T., Yousef, H. A., Dorrah, M. A., Mohamed, A. A., Duvic, B. J. Insect Sci. 2015, 15, 57; https://doi.org/10.1093/jisesa/iev038.Search in Google Scholar PubMed PubMed Central

45. Köse, K., Erol, K., Köse, D. A. Adsorption 2020, 26, 329–337, https://doi.org/10.1007%2Fs10450-020-00212-9.10.1007/s10450-020-00212-9Search in Google Scholar

46. Harrison, M. J., Burton, N. A., Hillier, I. H. J. Am. Chem. Soc. 1997, 119, 12285–12291; https://doi.org/10.1021/ja9711472.Search in Google Scholar

47. Park, J. M., Kim, M., Park, H.-S., Jang, A., Min, J., Kim, Y. H. Int. J. Biol. Macromol. 2013, 54, 37–43; https://doi.org/10.1016/j.ijbiomac.2012.11.025.Search in Google Scholar PubMed

48. Yılmaz, F., Bereli, N., Yavuz, H., Denizli, A. Biochem. Eng. J. 2009, 43, 272–279.10.1016/j.bej.2008.10.009Search in Google Scholar

49. Xue, F., Chen, Q., Li, Y., Liu, E., Li, D. Enzym. Microb. Technol. 2019, 131, 109425; https://doi.org/10.1016/j.enzmictec.2019.109425.Search in Google Scholar PubMed

50. Lian, Z. X., Ma, Z. S., Wei, J., Liu, H. Process Biochem. 2012, 47, 201–208; https://doi.org/10.1016/j.procbio.2011.10.031.Search in Google Scholar

51. Deng, Y., Li, J., Pu, Y., Chen, Y., Zhao, J., Tang, J. React. Funct. Polym. 2016, 103, 92–98; https://doi.org/10.1016/j.reactfunctpolym.2016.04.007.Search in Google Scholar

Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/polyeng-2020-0191).

Received: 2020-07-22
Accepted: 2020-10-29
Published Online: 2020-12-16
Published in Print: 2021-02-23

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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  4. Effect of blending procedures and reactive compatibilizers on the properties of biodegradable poly(butylene adipate-co-terephthalate)/poly(lactic acid) blends
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https://doi.org/10.1515/polyeng-2020-0191
Keywords for this article
activity; adsorption; enzyme immobilization; lysozyme; magnetic microparticle

Articles in the same Issue

  1. Frontmatter
  2. Material properties
  3. Thermoelastic characterization of carbon nanotube reinforced PDMS elastomer
  4. Effect of blending procedures and reactive compatibilizers on the properties of biodegradable poly(butylene adipate-co-terephthalate)/poly(lactic acid) blends
  5. The effects of morphological variation and polymer/polymer interface on the tensile modulus of binary polymer blends: a modeling approach
  6. Effect of gamma radiation on the structural, thermal and optical properties of PMMA/Sn0.75Fe0.25S2 nanocomposite
  7. Preparation and assembly
  8. Elaboration and characterization of multilayer polymeric membranes: effect of the chemical nature of polymers
  9. Fabrication and charge storage capacitance of PPY/TiO2/PPY jacket nanotube array
  10. Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
  11. Engineering and processing
  12. Influence of low-fracture-fiber mechanism on fiber/melt-flow behavior and tensile properties of ultra-long-glass-fiber-reinforced polypropylene composites injection molding
  13. Bilayer PMMA antireflective coatings via microphase separation and MAPLE
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