The effects of cross-linked/uncross-linked electrospun fibrinogen/polycaprolactone nanofibers on the proliferation of normal human epidermal keratinocytes
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Mohamad Javad Mirzaei-Parsa
Abstract
The aim of this study was an investigation on the proliferation rate of normal human epidermal keratinocytes (NHEK) on the cross-linked and uncross-linked fibrinogen/polycaprolactone (Fbg/PCL) nanofibers to determine a suitable scaffold for skin tissue engineering. Nanofibrous scaffolds were prepared by electrospinning of different weight ratios of Fbg to PCL and were analyzed as morphology, surface chemical properties and cytocompatibility by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, respectively. The diameters of the blended uncross-linked scaffolds were in the range of 124±43 nm–209±155 nm. Cross-linking of scaffolds with glutaraldehyde did not make a significant change in the diameter of blended scaffolds in 16 h. Cross-linking also improved the tensile strength and weight loss rate of scaffolds. However, cross-linking demonstrated an unfavorable effect on the attachment and proliferation of NHEK cells. The proliferation study revealed that uncross-linked scaffolds containing 50% and 70% Fbg provide a better environment for the growth of NHEK cells, and can be considered promising scaffolds in tissue engineering applications.
Acknowledgments
This project was supported by Tehran University of Medical Sciences (TUMS; grant no. 94-0287-28594).
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Material properties
- Steady shear and dynamic strain thickening of halloysite nanotubes and fumed silica shear thickening composite
- Diffusivity of solvents in semi-crystalline polyethylene using the Vrentas-Duda free-volume theory
- Toughening effect and mechanism of polyamide 12 and modified montmorillonite in polybenzoxazine resin
- The effects of cross-linked/uncross-linked electrospun fibrinogen/polycaprolactone nanofibers on the proliferation of normal human epidermal keratinocytes
- Frequency independent AC electrical conductivity and dielectric properties of polyaniline-based conductive thermosetting composite
- Preparation and assembly
- Study on the preparation and drug release property of soybean selenoprotein/carboxymethyl chitosan composite hydrogel
- Engineering and processing
- Interaction of nanofillers in injection-molded graphene/carbon nanotube reinforced PA66 hybrid nanocomposites
- Prediction of the yellowing of styrene-stat-acrylonitrile and acrylonitrile-butadiene-styrene during processing in an internal mixer
- Milling process optimization for the best surface coat adhesion of the rigid polyurethane foam
- A numerical analysis of calendering of Oldroyd 4-constant fluid
Articles in the same Issue
- Frontmatter
- Material properties
- Steady shear and dynamic strain thickening of halloysite nanotubes and fumed silica shear thickening composite
- Diffusivity of solvents in semi-crystalline polyethylene using the Vrentas-Duda free-volume theory
- Toughening effect and mechanism of polyamide 12 and modified montmorillonite in polybenzoxazine resin
- The effects of cross-linked/uncross-linked electrospun fibrinogen/polycaprolactone nanofibers on the proliferation of normal human epidermal keratinocytes
- Frequency independent AC electrical conductivity and dielectric properties of polyaniline-based conductive thermosetting composite
- Preparation and assembly
- Study on the preparation and drug release property of soybean selenoprotein/carboxymethyl chitosan composite hydrogel
- Engineering and processing
- Interaction of nanofillers in injection-molded graphene/carbon nanotube reinforced PA66 hybrid nanocomposites
- Prediction of the yellowing of styrene-stat-acrylonitrile and acrylonitrile-butadiene-styrene during processing in an internal mixer
- Milling process optimization for the best surface coat adhesion of the rigid polyurethane foam
- A numerical analysis of calendering of Oldroyd 4-constant fluid
