Abstract
In this study, the electro-kinetic phenomena (EKP) induced in biological tissue by external electric field, while not directly visible in optical coherence tomography (OCT) images, were detected by analyzing their textural speckle features. During application of a low-frequency electric field to the tissue, speckle patterns changed their brightness and shape depending on the local tissue EKP. Since intensities of OCT image speckle patterns were analyzed and discussed in our previous publications, this work is mainly focused on OCT signal phase analysis. The algorithm for extracting local spatial phase variations from unwrapped phases is introduced. The detection of electrically induced optical changes manifest in OCT phase images shows promise for monitoring the fixed charge density changes within tissues through their electro-kinetic responses. This approach may help in the identification and characterization of morphology and function of healthy and pathologic tissues.
Zusammenfassung
In dieser Studie wurden die elektrokinetischen Phänomene (EKP) in biologischem Gewebe, die durch ein äußeres elektrisches Feld induziert wurden, jedoch nicht direkt mittels optischer Kohärenztomographie (optical coherence tomography, OCT) dargestellt werden können, durch die Analyse ihrer strukturellen Speckle-Merkmale erfasst. Wie sich zeigte ändern die Speckle-Muster während der Anwendung eines niederfrequenten elektrischen Feldes ihre Helligkeit und Form in Abhängigkeit von den lokalen EKP des Gewebes. Während in unseren früheren Veröffentlichungen die Intensitäten der Speckle-Muster in den OCT-Bildern analysiert wurden, fokussiert sich die vorliegende Arbeit auf die Signal-Phasenanalyse und stellt einen entsprechenden Algorithmus zur Extraktion lokaler räumlicher Phasenvariationen vor.
Die Detektion von elektrisch induzierten optischen Veränderungen die sich in den OCT-Phasenbildern manifestieren scheint für das Monitoring fester Ladungsdichteveränderungen im Gewebe durch ihre elektrokinetische Reaktion vielversprechend. Dieser Ansatz kann bei der Identifizierung und Charakterisierung der Morphologie und Funktion von gesunden und pathologischen Geweben helfen.
Acknowledgments
This research was supported by the Discovery Grants Program of the Natural Sciences and Engineering Research Council of Canada (Y. Xu and V. Toronov), the Canada Foundation for Innovation (V. Yang), and the Mathematics of Information Technology and Complex Systems (B. Vuong and C. Sun).
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©2014 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
- Biophotonics symposium – A scientific cruise
- Editors’ note
- Reviewer acknowledgment
- Magazine section
- Snapshots
- Review
- Novel methods for elasticity characterization using optical coherence tomography: Brief review and future prospects
- Original contributions
- Correlating optical coherence tomography images with dose distribution in late oral radiation toxicity patients
- Optical coherence tomography in diagnosing inflammatory diseases of ENT
- Imaging the electro-kinetic response of biological tissues with phase-resolved optical coherence tomography
- Real-time clinical clutter reduction in combined epi-optoacoustic and ultrasound imaging
- Selection of stabilizing agents to provide effective penetration of gold nanoparticles into cells
- Preliminary research report
- Mechanical compression in cross-polarization OCT imaging of skin: In vivo study and Monte Carlo simulation
- Technical note
- High-precision terahertz spectroscopy for noninvasive medicine diagnostics
- Announcement
- Protokoll der Mitgliederversammlung der Deutschen Gesellschaft für Lasermedizin (DGLM) e.V.
- Congress announcements
- Congresses 2014/2015
- Contents of the Volume
- Contents of the Volume
Articles in the same Issue
- Frontmatter
- Editorial
- Biophotonics symposium – A scientific cruise
- Editors’ note
- Reviewer acknowledgment
- Magazine section
- Snapshots
- Review
- Novel methods for elasticity characterization using optical coherence tomography: Brief review and future prospects
- Original contributions
- Correlating optical coherence tomography images with dose distribution in late oral radiation toxicity patients
- Optical coherence tomography in diagnosing inflammatory diseases of ENT
- Imaging the electro-kinetic response of biological tissues with phase-resolved optical coherence tomography
- Real-time clinical clutter reduction in combined epi-optoacoustic and ultrasound imaging
- Selection of stabilizing agents to provide effective penetration of gold nanoparticles into cells
- Preliminary research report
- Mechanical compression in cross-polarization OCT imaging of skin: In vivo study and Monte Carlo simulation
- Technical note
- High-precision terahertz spectroscopy for noninvasive medicine diagnostics
- Announcement
- Protokoll der Mitgliederversammlung der Deutschen Gesellschaft für Lasermedizin (DGLM) e.V.
- Congress announcements
- Congresses 2014/2015
- Contents of the Volume
- Contents of the Volume