Development of a second harmonic generation microscope optimized for biomaterial studies
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Imee Su Martinez
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Allen Marbert S. Lee
Abstract
A Second harmonic generation (SHG) microscope optimized for biomaterials was developed. The set-up has a non-collinear counter propagating geometry, that allows for the polarization tuning of incoming and outgoing beams. The developed microscope has a resolution of 1.1 µm/line pair. It makes use of a highly sensitive and high-resolution CCD camera that allows for the imaging of delicate biomaterial samples in short acquisition times without burning. This is the first and only functional SHG microscope set-up in the Philippines.
Funding source: Philippine Council for Industry, Energy, and Emerging Technology Research and Development
Funding source: Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development
Award Identifier / Grant number: CHE-18-1-05
Funding source: Industrial Technology Development Institute
Funding source: Department of Science and Technology-Accelerated Science and Technology Human Resource Development Program
Funding source: University of the Philippines Diliman Office of the Vice Chancellor for Research and Development Outright Research Grant
Award Identifier / Grant number: ORG 232323
Acknowledgments
The authors would like to acknowledge funding provided by the Natural Sciences Research Institute (CHE-18-1-05), the Department of Science and Technology (DOST)–PCAARRD, DOST-PCIEERD, the DOST–Industrial Technology Development Institute, the DOST-Accelerated Science and Technology Human Resource Development Programꟷfor personnel support, and the OVCRD Outright Research Grant 232323. We would also like to acknowledge the generosity of Dr. Ricky Nellas for lending his upright microscope, and Dr. Maria Antonette Juinio-Meñez for providing the samples from the Marine Science Institute Bolinao Marine Laboratory.
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Research ethics: Not applicable.
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Informed consent: Not applicable.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission. Imee Su Martinez is the principal investigator of the study, and wrote the manuscript. Allen Marbert Lee built the first prototype of the SHG microscope in laboratory. Kent Andew Llamson optimized the microscope for biomaterials and improved the microscope by equipping it for future polarization mapping and chirality studies.
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Use of Large Language Models, AI and Machine Learning Tools: None declared.
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Conflict of interest: The authors state no conflict of interest.
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Research funding: Funding was provided by the Natural Sciences Research Institute (CHE-18-1-05), the Department of Science and Technology (DOST)–PCAARRD, DOST-PCIEERD, the DOST–Industrial Technology Development Institute, the DOST-Accelerated Science and Technology Human Resource Development Programꟷfor personnel support, and the OVCRD Outright Research Grant 232323.
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Data availability: Not applicable.
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Supporting Information: A video of dry collagen film second harmonic generation, as it was exposed to the atmosphere.
References
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Supplementary Material
This article contains supplementary material (https://doi.org/10.1515/pac-2024-0383).
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- Effect of chemical modification using glyoxylic acid on the stability of α-amylase from Aspergillus fumigatus
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