The karyotype of Trinomys paratus (Rodentia: Echimyidae) with comments about its phylogenetic relationship
-
Ana Lazar
,
Camila Nacif
Abstract
The spiny rat Trinomys, with ten endemic species in eastern Brazil, has a complex taxonomy. We carried out a revision of the karyotypes of Trinomys, described for the first time the karyotype of Trinomys paratus and performed the first phylogenetic analysis including all Trinomys species based on the mitochondrial cytochrome b gene. The T. paratus karyotype showed diploid number of 58 and fundamental autosomal number of 112. Diploid and fundamental autosomal numbers (FNa), and chromosomes’ morphology, are similar to those described for Trinomys eliasi. T. paratus appears as sister taxa of T. eliasi; in turn, this clade was recovered as the sister group of Trinomys setosus, as previously reported, confirming that sister species of Trinomys has conserved karyotypes, and suggesting that karyological evolution in this genus could be slower than species differentiation.
Acknowledgments
This work was supported by FAPERJ/CAPES (fellowship E26/102.804/2011) to AL, CNPq (grant nos. 307669/2013-0 and 440663/2015-6 to CRB and MW, respectively) and FAPERJ (grant no. E26/201/200/2014 to CRB). We are thankful to Dr. Paulo S. D’Andrea, Dr. Ana Jansen, and the field teams of the Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios and Laboratório de Biologia de Tripanosomatídeos IOC/ FIOCRUZ for collecting the specimens. Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) granted license to collect the specimens.
References
Anderson, S. and T.L. Yates. 2000. A new genus and species of phyllotine rodent from Bolivia. J. Mamm. 81: 18–36.10.1644/1545-1542(2000)081<0018:ANGASO>2.0.CO;2Suche in Google Scholar
Andrade, A.F.B. and C.R. Bonvicino. 2003. A new karyological variant of Oecomys (Rodentia: Sigmodontinae) and its phylogenetic relationship based on molecular data. Genome 46: 195–203.10.1139/g02-123Suche in Google Scholar
Cassens, I., S. Vicario, V.G. Waddell, H. Balchowsky, D. Van Belle, W. Ding, C. Fan, R.S. Mohan, P.C. Simões-Lopes, R. Bastida, A. Meyer, M.J. Stanhope and M.C. Milinkovitch. 2000. Independent adaptation to riverine habitats allowed survival of ancient cetacean lineages. Proc. Natl. Acad. Sci. USA 97: 11343–11347.10.1073/pnas.97.21.11343Suche in Google Scholar
Corrêa, M.M.O., M.O.G. Lopes, E.V.C. Câmara, L.C. Oliveira and L.M. Pessôa. 2005. The karyotypes of Trinomys moojeni (Pessôa, Oliveira and Reis, 1992) and Trinomys setosus elegans (Lund, 1841) (Rodentia, Echimyidae) from Minas Gerais, eastern Brazil. Arq. Mus. Nac. 63: 169–174.Suche in Google Scholar
Dalapicolla, J. and Y.L.R. Leite. 2015. Taxonomic implications of morphological variation in three species of Trinomys (Rodentia: Echimyidae) from eastern Brazil. Zootaxa, 3919: 61–80.10.11646/zootaxa.3919.1.3Suche in Google Scholar
Irwin, D.M., T.D. Kocher and A.C. Wilsom. 1991. Evolution of the cytochrome b gene of mammals. J. Mol. Evol. 32: 128–144.10.1007/BF02515385Suche in Google Scholar
Keane, T.M., C.J. Creevey, M.M. Pentony, T.J. Naughton and J.O. Mclnerney. 2006. Assessment of methods for amino acid matrix selection and their use on empirical data shows that ad hoc assumptions for choice of matrix are not justified. BMC Evol. Biol. 6: 29.10.1186/1471-2148-6-29Suche in Google Scholar
Lara, M.C. and J.L. Patton. 2000. Evolutionary diversification of spiny rats (genus Trinomys, Rodentia: Echimyidae) in the Atlantic Forest of Brazil. Zool. J. Linn. Soc. 130: 661–686.10.1111/j.1096-3642.2000.tb02205.xSuche in Google Scholar
Lara, M.C., J.L. Patton and M.N.F. da Silva. 1996. The simultaneous diversification of South American echimyid rodents (Hystrigonathi) based on complete cytochrome-b sequences. Mol. Phylogenet. Evol. 5: 403–13.10.1006/mpev.1996.0035Suche in Google Scholar
Leal- Mesquita, E.R., Y. Yonenaga-Yassuda, T.H. Chu and P.L.B. Rocha. 1992. Chromosomal characterization and comparative cytogenetic analysis of two species of Proechimys (Echimyidae, Rodentia) from the Caatinga domain of state of Bahia, Brazil. Caryologia 45: 197–212.10.1080/00087114.1992.10797223Suche in Google Scholar
Moojen, J. 1948. Speciation in the Brazilian spiny rats (genus Proechimys, family Echimyidae). Univ. Kansas Publ., Mus. Nat. Hist. 1: 301–406.Suche in Google Scholar
Nascimento, F.F., A. Lazar, A.N. Menezes, A.M. Durans, J.C. Moreira, J. Salazar- Bravo, P.S. D’Andrea and C.R. Bonvicino. 2013. The role of historical barriers in the diversification process in open vegetation formations during the Miocene/Pliocene using an ancient rodent lineage as a model. PLoS One 8: e61924.10.1371/journal.pone.0061924Suche in Google Scholar
Paresque, R., W.P. Souza, S.L. Mendes and V. Fagundes. 2004. Composicão cariotípica da fauna de roedores e marsupiais de duas áreas de Mata Atlântica do Espírito Santo state, Brazil. Bol. Mus. Biol. Mello Leitão, nova serie 17: 5–33.Suche in Google Scholar
Pessôa, L.M. and S.F. Reis. 1993. A new subspecies of Proechimys iheringi Thomas (Rodentia: Echimyidae) from the State of Rio de Janeiro, Brazil. Z. Säugetierk. 58: 181–90.Suche in Google Scholar
Pessôa, L.M., J.A. Oliveira and S.F. Reis. 1992. A new species of Spiny rat genus Proechimys, subgenus Trinomys (Rodentia: Echimyidae). Z. Säugetierk. 57: 39–46.Suche in Google Scholar
Pessôa, L.M., M.M.O. Corrêa, E. Bittencourt and S.F. Reis. 2005. Chromosomal characterization of taxa of the genus Trinomys Thomas, 1921, in states of Rio de Janeiro and São Paulo (Rodentia: Echimyidae). Arq. Mus. Nac. 63: 161–68.Suche in Google Scholar
Pessôa, L.M., W.C. Tavares, J.A. Oliveira and J.L. Patton. 2015. Genus Trinomys. In: (J.L. Patton, U.F.J. Pardiñas and G. D’Elía, eds.) Mammals of South America. Vol. 2. Rodents. University of Chicago Press, Chicago, IL, pp. 999–1019.Suche in Google Scholar
Posada, D. and K.A. Crandall. 2001. Selecting the best-fit model of nucleotide substitution. Syst. Biol. 50: 580–601.10.1080/106351501750435121Suche in Google Scholar
Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Suche in Google Scholar
Stamatakis, A. 2006. RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.10.1093/bioinformatics/btl446Suche in Google Scholar
Souza, A.L.G., M.M.O. Corrêa and L.M. Pessôa. 2006. Morphometric discrimination between Trinomys albispinus (Is. Geoffroy, 1838) and Trinomys minor (Reis & Pessôa, 1995) from Chapada Diamantina, Bahia, Brazil, and the karyotype of Trinomys albispinus (Rodentia, Echimyidae). Arq. Mus. Nac. 64: 325–332.Suche in Google Scholar
Sumner, A.T. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp. Cell. Res. 75: 304–306.10.1016/0014-4827(72)90558-7Suche in Google Scholar
Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30: 2725–2729.10.1093/molbev/mst197Suche in Google Scholar PubMed PubMed Central
Tavares, W.C. and L.M. Pessôa. 2010. Variação morfológica em populações de Trinomys (Thomas, 1921) de restingas e matas de baixada no estado do Rio de Janeiro. In: (L.M. Pessôa, W.C. Tavares and S. Siciliano, eds.) Mamíferos de restinga e manguezais do Brasil. Sociedade Brasileira de Mastozoologia, Rio de Janeiro, Série Livros 1, pp. 127–154.Suche in Google Scholar
Tavares, W.C., L.M. Pessôa and H.N. Seuánez. 2015. Plio-Pleistocene history of the endangered spiny rat Trinomys eliasi (Echimyidae) from Rio de Janeiro, Brazil. J. Mamm. 96: 94–106.10.1093/jmammal/gyu010Suche in Google Scholar
Tavares, W.C., L.M. Pessôa and H.N. Seuánez. 2016. Phylogenetic and size constrains on cranial ontogenetic allometry of spiny rats (Echimyidae, Rodentia). J. Evol. Biol. 29: 1752–1765.10.1111/jeb.12905Suche in Google Scholar PubMed
Upham, N.S. and B.D. Patterson. 2012. Diversification and biogeography of the Neotropical caviomorph lineage Octodontoidea (Rodentia: Hystricognathi). Mol. Phylogenet. Evol. 63: 417–429.10.1016/j.ympev.2012.01.020Suche in Google Scholar PubMed
Yonenaga-Yassuda, Y., M.J. Souza, S. Kasahara, M. L’abbate and H.T. Chu. 1985. Supernumerary system in Proechimys iheringi iheringi (Rodentia, Echimydae) from the State of São Paulo, Brazil. Caryologia 38: 179–94.10.1080/00087114.1985.10797742Suche in Google Scholar
Zanchin, N.I., 1988. Estudos cromossômicos em orizominos e equimídeos da Mata Atlântica. Master’s thesis, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.Suche in Google Scholar
©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Studies
- Home-range size of the European wildcat (Felis silvestris silvestris): a report from two areas in Central Italy
- An SEM image reference guide to hairs of 12 species of large African mammals
- Habitat and food utilization by banteng (Bos javanicus d’Alton, 1823) accidentally introduced into the Khao Khieo-Khao Chomphu Wildlife Sanctuary, Thailand
- Integrative lineage delimitation in rodents of the Ctenomys Corrientes group
- Surface foraging in Scapanus moles
- Short Notes
- Home range of a male jaguar spatially associated with the landfill of the city of Playa del Carmen, Mexico
- Resting sites of opossums (Didelphimorphia, Didelphidae) in Atlantic Forest fragments
- Bats found entangled in natural and artificial traps
- Round-pot feeder: low-cost apparatus for field studies on food supplementation for arboreal small mammals
- Distributional extensions of Carollia castanea and Micronycteris minuta from Guatemala, Central America
- Albinism in the striped spear-nosed bat Gardnerycteris crenulatum (Chiroptera: Phyllostomidae) with an updated list of albino bats in the World
- A new mammal species for Algeria, Rhinopoma microphyllum (Chiroptera: Rhinopomatidae): morphological and acoustic identification
- The taxonomic status of Copemyodon ecuadorensis (Rodentia, Cricetidae), a supposedly extinct muroid from the Ecuadorean Quaternary
- The karyotype of Trinomys paratus (Rodentia: Echimyidae) with comments about its phylogenetic relationship
- Taxonomic status of Wiedomys marplatensis, an enigmatic fossil cricetid (Rodentia, Sigmodontinae): neither Wiedomys nor Wiedomyini
Artikel in diesem Heft
- Frontmatter
- Original Studies
- Home-range size of the European wildcat (Felis silvestris silvestris): a report from two areas in Central Italy
- An SEM image reference guide to hairs of 12 species of large African mammals
- Habitat and food utilization by banteng (Bos javanicus d’Alton, 1823) accidentally introduced into the Khao Khieo-Khao Chomphu Wildlife Sanctuary, Thailand
- Integrative lineage delimitation in rodents of the Ctenomys Corrientes group
- Surface foraging in Scapanus moles
- Short Notes
- Home range of a male jaguar spatially associated with the landfill of the city of Playa del Carmen, Mexico
- Resting sites of opossums (Didelphimorphia, Didelphidae) in Atlantic Forest fragments
- Bats found entangled in natural and artificial traps
- Round-pot feeder: low-cost apparatus for field studies on food supplementation for arboreal small mammals
- Distributional extensions of Carollia castanea and Micronycteris minuta from Guatemala, Central America
- Albinism in the striped spear-nosed bat Gardnerycteris crenulatum (Chiroptera: Phyllostomidae) with an updated list of albino bats in the World
- A new mammal species for Algeria, Rhinopoma microphyllum (Chiroptera: Rhinopomatidae): morphological and acoustic identification
- The taxonomic status of Copemyodon ecuadorensis (Rodentia, Cricetidae), a supposedly extinct muroid from the Ecuadorean Quaternary
- The karyotype of Trinomys paratus (Rodentia: Echimyidae) with comments about its phylogenetic relationship
- Taxonomic status of Wiedomys marplatensis, an enigmatic fossil cricetid (Rodentia, Sigmodontinae): neither Wiedomys nor Wiedomyini
