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Application of denaturing high-performance liquid chromatography for intestinal microbiota analysis of newborns
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Gaëlle Caillaux
Published/Copyright:
February 2, 2010
Abstract
Aims: Neonatal microbiota development seems to play a key role in the early origins of health and disease. However, the analysis of this complex ecosystem is still difficult. The aim of this work was to investigate the feasibility of a new technique, denaturing high-performance liquid chromatography (dHPLC), to analyze newborn intestinal microbiota using genomic approaches.
Methods and results: Eleven neonates were recruited among patients admitted for intestinal surgery to the neonatal intensive care unit. Preoperative samplings were obtained in each case. Three methodologies were compared for each sample: (i) dHPLC, (ii) temporal temperature gradient gel electrophoresis (TTGE), and (iii) conventional culture techniques.
Results: All samples were poorly colonized. In three samples, the microbiota was detected only with the dHPLC technique. Results obtained with culture and TTGE could be found with dHPLC.
Conclusion: The results suggest that neonatal applications of the dHPLC technique, especially for gut microbiota analysis, appear to be a sensitive and promising analytical technique.
Received: 2009-8-3
Revised: 2009-9-8
Accepted: 2009-10-7
Published Online: 2010-02-02
Published Online: 2010-02-2
Published in Print: 2010-05-01
©2010 by Walter de Gruyter Berlin New York
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Articles in the same Issue
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- Recommendations and guidelines for perinatal practice
- Optimization of human milk fortification for preterm infants: new concepts and recommendations
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- Effect of maternal clinical chorioamnionitis on neonatal morbidity in very-low birthweight infants: a case-control study
- The frequency and clinical significance of intra-amniotic infection and/or inflammation in women with placenta previa and vaginal bleeding: an unexpected observation
- Evidence for differential regulation of the adipokine visfatin in the maternal and fetal compartments in normal spontaneous labor at term
- Original articles – Fetus
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- Congress Calender
- Congress Calendar
- Announcement
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