The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia
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Franziska A. Stressmann
Abstract
Although premature infants are increasingly surviving the neonatal period, up to one-third develop bronchopulmonary dysplasia (BPD). Despite evidence that bacterial colonization of the neonatal respiratory tract by certain bacteria may be a risk factor in BPD development, little is known about the role these bacteria play. The aim of this study was to investigate the use of culture-independent molecular profiling methodologies to identify potential etiological agents in neonatal airway secretions. This study used terminal restriction fragment length polymorphism (T-RFLP) and clone sequence analyses to characterize bacterial species in endo-tracheal (ET) aspirates from eight intubated pre-term infants. A wide range of different bacteria was identified in the samples. Forty-seven T-RF band lengths were resolved in the sample set, with a range of 0–15 separate species in each patient. Clone sequence analyses confirmed the identity of individual species detected by T-RFLP. We speculate that the identification of known opportunistic pathogens including S. aureus, Enterobacter sp., Moraxella catarrhalis, Pseudomonasaeruginosa and Streptococcus sp., within the airways of pre-term infants, might be causally related to the subsequent development of BPD. Further, we suggest that culture-independent techniques, such as T-RFLP, hold important potential for the characterization of neonatal conditions, such as BPD.
©2010 by Walter de Gruyter Berlin New York
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- Original articles – Newborn
- Avoidable mortality in small-for-gestational-age children in the Netherlands
- Incidence of perinatal complications in children with premature craniosynostosis
- Refractive status and ocular axial length in preterm infants without retinopathy of prematurity with regard to birth weight and gestational age
- The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia
- Application of denaturing high-performance liquid chromatography for intestinal microbiota analysis of newborns
- Errata
- Errata
- Congress Calender
- Congress Calendar
- Announcement
- Announcement
Articles in the same Issue
- Editorial
- Recommendations and guidelines for perinatal practice
- Optimization of human milk fortification for preterm infants: new concepts and recommendations
- Review articles
- Fetal growth restriction – from observation to intervention
- From monster to twin reversed arterial perfusion: a history of acardiac twins
- Original articles – Obstetrics
- The relationship between maternal insulin-like growth factors 1 and 2 (IGF-1, IGF-2) and IGFBP-3 to gestational age and preterm delivery
- Detection of ureaplasmas by the polymerase chain reaction in the amniotic fluid of patients with cervical insufficiency
- Effect of maternal clinical chorioamnionitis on neonatal morbidity in very-low birthweight infants: a case-control study
- The frequency and clinical significance of intra-amniotic infection and/or inflammation in women with placenta previa and vaginal bleeding: an unexpected observation
- Evidence for differential regulation of the adipokine visfatin in the maternal and fetal compartments in normal spontaneous labor at term
- Original articles – Fetus
- Birth weight percentile charts based on daily measurements for very preterm male and female infants at the age of 154–223 days
- Increasing rates of preterm twin births coincide with improving twin pair survival
- Estimating the effect of gestational age on test performance of combined first-trimester screening for Down syndrome: a preliminary study
- Original articles – Newborn
- Avoidable mortality in small-for-gestational-age children in the Netherlands
- Incidence of perinatal complications in children with premature craniosynostosis
- Refractive status and ocular axial length in preterm infants without retinopathy of prematurity with regard to birth weight and gestational age
- The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia
- Application of denaturing high-performance liquid chromatography for intestinal microbiota analysis of newborns
- Errata
- Errata
- Congress Calender
- Congress Calendar
- Announcement
- Announcement
