Metabolic characterization of amniotic fluid of fetuses with isolated choroid plexus cyst

Shangqing Li; Guorong Lyu; Shaohui Li; Hainan Yang; Yiru Yang

doi:10.1515/jpm-2022-0028

Article

Metabolic characterization of amniotic fluid of fetuses with isolated choroid plexus cyst

Shangqing Li , Guorong Lyu , Shaohui Li , Hainan Yang and Yiru Yang

Published/Copyright: May 25, 2022

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From the journal Journal of Perinatal Medicine Volume 50 Issue 8

Abstract

Objectives

To investigate the amino acid (AA)-related metabolic characteristics of amniotic fluid (AF) obtained by ultrasound-guided amniocentesis from fetuses with isolated choroid plexus cysts of the central nervous system.

Methods

Ultrasound-guided amniocentesis was performed on 17 fetuses with isolated choroid plexus cysts (ICPCs) and 17 normal fetuses. The AF samples from normal pregnancies were matched with the case samples in a 1:1 ratio based upon gestational age. The AF samples from the 34 fetuses were analyzed by liquid chromatography–mass spectrometry (LC–MS). Then, the peak areas of the metabolites were analyzed by principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and univariate statistical analysis.

Results

This study ultimately identified 31 AAs. Seven differentially abundant AAs were screened out, including citrulline, ethanolamine, aspartic acid, valine, 5-hydroxylysine, proline, and isoleucine (p-value<0.05). A total of 4 metabolic pathways were significantly altered in the ICPC group: valine, leucine and isoleucine biosynthesis; valine, leucine and isoleucine degradation; pantothenate and coenzyme A (CoA) biosynthesis; and arginine biosynthesis.

Conclusions

The results of this study indicate that fetuses with ICPC have disrupted levels of citrulline, ethanolamine, aspartic acid, valine, 5-hydroxylysine, proline, and isoleucine, which may ultimately affect fetal glucose and lipid metabolism.

Keywords: amino acid; amniotic fluid; choroid plexus cyst; metabolism

Corresponding author: Guorong Lyu, Department of Ultrasound, The Second Affiliated Hospital of Fujian Medical University, No. 2 Anji Road, Luojiang District, Quanzhou, Fujian, P.R. China; and Quanzhou Medical College, Quanzhou, Fujian, P.R. China, E-mail: lgr_feus@sina.com

Shangqing Li and Guorong Lyu contributed equally to this work and should be considered joint co-first authors.

Funding source: Fujian Natural Science Foundation

Award Identifier / Grant number: 2020J01129

Research funding: The present study was supported by the Fujian Natural Science Foundation (grant no. 2020J01129).
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: This study was reviewed by the Second Ethics Committee Affiliated to Fujian Medical University (Quanzhou, China).

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Received: 2022-01-18

Accepted: 2022-01-26

Published Online: 2022-05-25

Published in Print: 2022-10-26

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Articles in the same Issue

https://doi.org/10.1515/jpm-2022-0028

Keywords for this article

amino acid; amniotic fluid; choroid plexus cyst; metabolism