Omega-3 polyunsaturated fatty acids enhance cytokine production and oxidative stress in a mouse model of preterm labor
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Tharwat Stewart Boulis
, Burton Rochelson , Olivia Novick , Xiangying Xue , Prodyot K. Chatterjee , Madhu Gupta , Malvika H. Solanki , Meredith Akerman and Christine N. Metz
Abstract
Objective: Omega-3 polyunsaturated fatty acid (ω-3 PUFA) supplementation during pregnancy remains controversial. We sought to examine the effects of ω-3 PUFA on inflammation and oxidative stress in vitro and in vivo using a model of preterm labor.
Methods: In vivo. Female Swiss Webster mice were fed a normal diet or a 5% fish oil (FO) diet for 3 weeks then mated with normal-fed males. On gestational day 15, dams were injected with either saline (n=10 per group) or lipopolysaccharide (LPS, intrauterine) (n=10 per group). Maternal plasma, amniotic fluid, placentas, and uteri were collected 4 h later and assessed for cytokines; maternal plasma and amniotic fluids were analyzed for oxidative stress. In vitro. RAW264.7 mouse macrophage-like cells were treated with either: vehicle, H2O2, docosahexaenoic acid (DHA), or eicosapentaenoic acid (EPA) (0, 0.1–100 μM) and analyzed for oxidative stress.
Results: In vivo. Administration of the 5% FO diet enhanced LPS-induced cytokines in the placenta (P<0.05–0.01) and increased tumor necrosis factor-α in the uterus (P<0.05) and amniotic fluid (P<0.01) when compared to LPS-treated normal-fed animals. Maternal plasma obtained from FO-fed dams showed higher LPS-induced oxidative stress than control-fed animals (P<0.035). However, no differences in oxidative stress were observed in the amniotic fluid. In vitro. Treatment of macrophage-like cells with ω-3 PUFA significantly and dose-dependently increased oxidative stress (P<0.001–0.0001).
Conclusions: Supplementation with FO for prior to and during pregnancy significantly increased LPS-induced inflammation in the amniotic fluid, uterus, and placenta and significantly increased maternal systemic oxidative stress in vivo. Likewise, DHA and EPA induced oxidative stress in macrophage-like cells in vitro.
References
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©2014 by De Gruyter
Articles in the same Issue
- Frontmatter
- Guest editorial
- Guest editorial
- Original articles
- Identification and isolation of putative stem cells from the murine placenta
- Target versus actual oxygenation index at initiation of inhaled nitric oxide in neonates with hypoxic respiratory failure: survey results from 128 patient cases
- Omega-3 polyunsaturated fatty acids enhance cytokine production and oxidative stress in a mouse model of preterm labor
- Adrenocorticotropic hormone and cortisol levels in term infants born with meconium-stained amniotic fluid
- Measurement of novel biomarkers of neuronal injury and cerebral oxygenation after routine vaginal delivery versus cesarean section in term infants
- The time-associated impact of the Newborn Influenza Protection Act on infant influenza rates in New York State
- Delivery-related knowledge of mothers of NICU infants compared with well-baby-nursery infants
- Review article
- Mercury exposure in pregnancy: a review
- Original articles - Obstetrics
- A “multi-hit” model of neonatal white matter injury: cumulative contributions of chronic placental inflammation, acute fetal inflammation and postnatal inflammatory events
- Balloon tamponade for the management of postpartum uterine hemorrhage
- The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study
- Obstetric anal sphincter injury risk reduction: a retrospective observational analysis
- Adverse perinatal outcomes related to the delivery mode in women with monochorionic diamniotic twin pregnancies
- Letter to the Editor
- The factors associated with the failure of transcatheter pelvic arterial embolization for intractable postpartum hemorrhage
- Congress Calendar
- Congress Calendar
Articles in the same Issue
- Frontmatter
- Guest editorial
- Guest editorial
- Original articles
- Identification and isolation of putative stem cells from the murine placenta
- Target versus actual oxygenation index at initiation of inhaled nitric oxide in neonates with hypoxic respiratory failure: survey results from 128 patient cases
- Omega-3 polyunsaturated fatty acids enhance cytokine production and oxidative stress in a mouse model of preterm labor
- Adrenocorticotropic hormone and cortisol levels in term infants born with meconium-stained amniotic fluid
- Measurement of novel biomarkers of neuronal injury and cerebral oxygenation after routine vaginal delivery versus cesarean section in term infants
- The time-associated impact of the Newborn Influenza Protection Act on infant influenza rates in New York State
- Delivery-related knowledge of mothers of NICU infants compared with well-baby-nursery infants
- Review article
- Mercury exposure in pregnancy: a review
- Original articles - Obstetrics
- A “multi-hit” model of neonatal white matter injury: cumulative contributions of chronic placental inflammation, acute fetal inflammation and postnatal inflammatory events
- Balloon tamponade for the management of postpartum uterine hemorrhage
- The IgG avidity value for the prediction of congenital cytomegalovirus infection in a prospective cohort study
- Obstetric anal sphincter injury risk reduction: a retrospective observational analysis
- Adverse perinatal outcomes related to the delivery mode in women with monochorionic diamniotic twin pregnancies
- Letter to the Editor
- The factors associated with the failure of transcatheter pelvic arterial embolization for intractable postpartum hemorrhage
- Congress Calendar
- Congress Calendar