Abstract
Aim: Infection-induced inflammation is a common cause of preterm birth. Pharmacologic inhibition of proinflammatory cytokines improves pregnancy outcome in animal models but there are no universally effective therapies for preterm birth in women. Carbon monoxide (CO) has anti-inflammatory properties at low concentrations but its effects on reproductive tissues is unclear. Therefore, we studied the effect of supplemental CO on the production of cytokines associated with preterm birth by fetal membranes.
Methods: Cross-sections of whole fetal membranes, isolated choriodecidua, and isolated amnion were prepared using tissues collected from women who had normal vaginal deliveries at term. Tissues were placed in an organ explant culture system and stimulated with up to 108 CFU/mL Escherichia coli. Cultures were incubated under room air or room air+250 ppm CO for 18 h and cytokine concentrations in conditioned medium were quantified by ELISA.
Results: CO inhibited IL-1β and TNF-α (P≤0.001) production by cultures stimulated with 107 CFU/mL bacteria but had no detectable effect on IL-10 by full-thickness membranes. Although CO also tended to reduce TNF-α production (P=0.053), no effect of CO was detected for IL-10 or IL-1β for membranes stimulated with 108 CFU/mL E. coli. TNF-α, but not IL-1β or IL-10 production, was inhibited by CO for choriodecidual cultures stimulated with 107 or 108 CFU/mL E. coli (P<0.001). IL-1β production was significantly inhibited by CO for amnion cultures stimulated with 107 (P=0.002) and 108 (P=0.017) CFU/mL E. coli. Exposure to bacteria had no effect on TNF-α or IL-10 production but CO tended to increase IL-10 production by amnion cultures stimulated with 108 CFU/mL E. coli (P=0.037).
Conclusions: These results suggest that CO may help promote an anti-inflammatory environment during intrauterine infections by inhibiting TNF-α and IL-1β production.
References
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The authors stated that there are no conflicts of interest regarding the publication of this article.
©2013 by Walter de Gruyter Berlin Boston
Articles in the same Issue
- Masthead
- Masthead
- Editorial
- Deliberative clinical ethical judgment: an essential component of contemporary obstetrics
- Review article
- Psychosocial stress in pregnancy and preterm birth: associations and mechanisms
- Opinion paper
- Management of prelabour rupture of membranes (PROM) at term
- Replies to Opinion paper
- Reply to “Management of prelabour rupture of membranes (PROM) at term”
- Reply to: Ismail AQT, Lahiri S. Management of prelabor rupture of membranes (PROM) at term. J Perinat Med. 2013
- Original articles – Obstetrics
- Treatment of PPROM with anhydramnion in humans: first experience with different amniotic fluid substitutes for continuous amnioinfusion through a subcutaneously implanted port system
- Characterization of the myometrial transcriptome in women with an arrest of dilatation during labor
- Does carbon monoxide inhibit proinflammatory cytokine production by fetal membranes?
- Mode of delivery at periviable gestational ages: impact on subsequent reproductive outcomes
- Commentary
- Mode of delivery at periviable gestational ages: impact on subsequent reproductive outcomes
- Original articles – Obstetrics
- Is pathologic confirmation of placental abruption more reliable in cases due to chronic etiologies compared with acute etiologies?
- Endouterine hemostatic square suture vs. Bakri balloon tamponade for intractable hemorrhage due to complete placenta previa
- Double exposure to intra-amniotic lipopolysaccharide and maternal betamethasone induces sustained increase of neutrophils in the lungs and disrupts alveolarization in newborn rats
- Nitrous oxide for analgesia in external cephalic version at term: prospective comparative studya
- Original articles – Fetus
- Development and application of an automated extraction algorithm for fetal magnetocardiography – normal data and arrhythmia detection
- Original articles – Newborn
- Growth of very low birth weight infants after increased amino acid and protein administration
- Congress Calendar
- Congress Calendar
Articles in the same Issue
- Masthead
- Masthead
- Editorial
- Deliberative clinical ethical judgment: an essential component of contemporary obstetrics
- Review article
- Psychosocial stress in pregnancy and preterm birth: associations and mechanisms
- Opinion paper
- Management of prelabour rupture of membranes (PROM) at term
- Replies to Opinion paper
- Reply to “Management of prelabour rupture of membranes (PROM) at term”
- Reply to: Ismail AQT, Lahiri S. Management of prelabor rupture of membranes (PROM) at term. J Perinat Med. 2013
- Original articles – Obstetrics
- Treatment of PPROM with anhydramnion in humans: first experience with different amniotic fluid substitutes for continuous amnioinfusion through a subcutaneously implanted port system
- Characterization of the myometrial transcriptome in women with an arrest of dilatation during labor
- Does carbon monoxide inhibit proinflammatory cytokine production by fetal membranes?
- Mode of delivery at periviable gestational ages: impact on subsequent reproductive outcomes
- Commentary
- Mode of delivery at periviable gestational ages: impact on subsequent reproductive outcomes
- Original articles – Obstetrics
- Is pathologic confirmation of placental abruption more reliable in cases due to chronic etiologies compared with acute etiologies?
- Endouterine hemostatic square suture vs. Bakri balloon tamponade for intractable hemorrhage due to complete placenta previa
- Double exposure to intra-amniotic lipopolysaccharide and maternal betamethasone induces sustained increase of neutrophils in the lungs and disrupts alveolarization in newborn rats
- Nitrous oxide for analgesia in external cephalic version at term: prospective comparative studya
- Original articles – Fetus
- Development and application of an automated extraction algorithm for fetal magnetocardiography – normal data and arrhythmia detection
- Original articles – Newborn
- Growth of very low birth weight infants after increased amino acid and protein administration
- Congress Calendar
- Congress Calendar