Is BaF3 Bioassay Useful to Identify Patients with Bioinactive Growth Hormone?
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Sara Pagani
ABSTRACT
We analyzed the ability of the BaF3 cell line bioassay to select patients with biologically inactive GH. We first evaluated the biological response of the Ba/F3-hGHR cells to rhGH additional doses from 10 to 5000 pg/ml. The concentration points corresponding to the linear part of the curve were selected. We then analyzed a group of sera, diluted like the standard, including the entire range of GH concentrations that can be analyzed by bioassay. The serum/standard area below the curve ratio was calculated. Serum GH immunoactivity determined by IMMULITE/GH bioactivity ratios was calculated. Our experimental data showed that GH-bioactivity/GH-immunoactivity ratios below 0.303 are indicative of a bioinactive GH molecule. This bioassay would recognize only extreme cases of GH bioinactivity, and it would not be a useful tool in the search for patients with altered forms of GH.
© Freund Publishing House Ltd. 2010
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- Selection of Elite Athletes, Hormones and Genes
- Association of Serum Adiponectin Levels with Artherosclerosis and the Metabolic Syndrome in Obese Children
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- Genital Surgery for Disorders of Sex Development: Implementing a Shared Decision-Making Approach
- Mean Platelet Volume in Obese Adolescents with Nonalcoholic Fatty Liver Disease
- Congenital Hypothyroidism: Etiology
- Familial Hyperinsulinism-Hyperammonemia Syndrome in a Family with Seizures: Case Report
- Genital Sanguineous Discharge in Prepuberty: A Case of Mullerian Papilloma of Vagina in a 9 Year-Old Girl
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- McCune-Albright Syndrome (MAS): Early and Extensive Bone Fibrous Dysplasia Involvement and “Mistaken Identity” Oophorectomy
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