Association of the apoptotic marker APO1/Fas with children’s predisposing factors for metabolic syndrome and with mean platelet volume

Maria Efthymia Katsa; Eirini Kostopoulou; Maria Magana; Anastasios Ioannidis; Stylianos Chatzipanagiotou; Athanasios Sachlas; Ioannis Dimopoulos; Bessie E. Spiliotis; Andrea Paola Rojas Gil

doi:10.1515/jpem-2021-0352

Article

Association of the apoptotic marker APO1/Fas with children’s predisposing factors for metabolic syndrome and with mean platelet volume

Maria Efthymia Katsa , Eirini Kostopoulou , Maria Magana , Anastasios Ioannidis , Stylianos Chatzipanagiotou , Athanasios Sachlas , Ioannis Dimopoulos , Bessie E. Spiliotis and Andrea Paola Rojas Gil

Published/Copyright: August 2, 2021

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From the journal Journal of Pediatric Endocrinology and Metabolism Volume 34 Issue 11

Abstract

Background

Apoptosis antigen 1/FAS receptor (APO1/Fas) signaling in endothelial cells plays a significant role in angiogenesis while increased mean platelet volume (MPV) is an important marker for platelet activation. We investigated the possible correlation between APO1/Fas and both metabolic parameters and platelet activity (indicated by the MPV) in a healthy pediatric population.

Methods

One hundred and eighty-five children, aged 5–17 years old, were enrolled in the study. The participants were divided into subgroups according to their age and body mass index percentile (BMI%). APO1/Fas was measured by enzyme-linked immunosorbent assay (ELISA) and MPV by the MEK-6410K.

Results

Eighty-one children (43.8%) had excess weight, which was more prevalent in children ≤9 years of age. Sixty-five children (35.1%) exhibited a predisposition for metabolic syndrome. A negative correlation was found between APO1/Fas and predisposing factors for metabolic syndrome: Glucose, cholesterol, uric acid, low-density lipoprotein (LDL), and triglycerides. In contrast, a positive correlation was found between APO1/Fas and C-reactive protein (CRP). Receiver operating characteristic (ROC) analysis showed a predisposition to metabolic syndrome when APO1/Fas was <78.46 pg/mL. A negative correlation was also observed between APO1/Fas and MPV. MPV was also positively correlated with predisposing factors for metabolic syndrome: BMI%, glucose, cholesterol, uric acid, LDL, and negatively with high-density lipoprotein.

Conclusions

APO1/Fas expression is associated with a lower predisposition to metabolic syndrome may be through endothelial homeostasis, the induction of apoptosis of cells involved in atherosclerosis, and platelet activity. It may also enhance CRP-mediated noninflammatory clearance of apoptotic cells. Early monitoring of all the components of metabolic syndrome in overweight children is important in order to prevent metabolic and cardiovascular complications.

Keywords: APO1/Fas; apoptosis; children; metabolic syndrome; MPV; obesity

Corresponding author: Andrea Paola Rojas Gil, Laboratory of Biology and Biochemistry, Faculty of Health Sciences, Department of Nursing, University of Peloponnese, Tripoli, Greece, Phone: +30 2710230125, E-mail: apaola71@yahoo.com.mx

Research funding: None declared.
Author contribution: Maria Efthymia Katsa, Maria Magana, and Andrea Paola Rojas Gil conceived/designed the original experiments, performed the experiments, analyzed the data of the original studies, and wrote the first draft of the manuscript. Athanasios Sachlas and Ioannis Dimopoulos performed the statistical analysis. Anastasios Ioannidis contributed to the design of original experiments and checked the statistical analysis of the data. Stylianos Chatzipanagiotou contributed to the realization of the experiments. Bessie E. Spiliotis and Eirini Kostopoulou contributed to the writing of the final manuscript.
Competing interests: The authors declare that they have no conflict of interest.
Informed consent: Informed consent was obtained from the parents and/or guardians.
Ethical approval: Hellenic Ministry of Education, Department of Healthcare and Environmental Education approved the survey, which was conducted in accordance with the declaration of Helsinki in 1975, as revised in 1983.

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Received: 2021-05-19

Revised: 2021-06-30

Accepted: 2021-07-12

Published Online: 2021-08-02

Published in Print: 2021-11-25

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Articles in the same Issue

https://doi.org/10.1515/jpem-2021-0352

Keywords for this article

APO1/Fas; apoptosis; children; metabolic syndrome; MPV; obesity