Birth weight related blood concentrations of the neurotransmission amino acids glutamine plus glutamate, phenylalanine and tyrosine in full-term breastfed infants perinatally
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Penelope D. Manta-Vogli
Abstract
Background
The amino acids glutamine plus glutamate, phenylalanine and tyrosine are implicated in neurotransmission. We aimed to evaluate these amino acid blood concentrations in full-term breastfed infants with different birth weight (BW) perinatally.
Methods
Breastfed full-term infants (n = 6000, males 3000, females 3000) BW 2000–4000 g were divided into four equal groups. Both males and females Groups A, 2000–2500 g, B 2500–3000 g, C 3000–3500 g, D 3500–4000 g. Blood samples on Guthrie cards, were taken on the 3rd day of life and quantified via a liquid chromatography tandem mass spectrometry (LC-MS/MS) method.
Results
Glutamine plus glutamate mean values were found to be statistically significantly different between males vs. females in all the studied groups. The highest values were determined in both males and females in group D. Statistically significantly higher values of phenylalanine appeared in group D vs. other groups. Tyrosine mean values were calculated to be statistically significantly different in both sexes in group A compared to other groups.
Conclusions
Differences of glutamine plus glutamate, phenylalanine and tyrosine levels among full-term newborns with different BW are presented for the first time in the literature. Newborns with BW 3000–4000 g are benefited by having higher concentrations of the mentioned neurotransmission related amino acids. Neonatal screening reference values for these amino acids in relation to BW could be established, not only for preterm and low BW infants but also for full-term newborns with BW >3000 g.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: None declared.
References
1. Bröer S, Bröer A. AA homeostasis and signaling in mammalian cells and organisms. Biochem J 2017;474:1935–63.10.1042/BCJ20160822Search in Google Scholar PubMed PubMed Central
2. Manta-Vogli PD, Schulpis KH, Dotsikas Y, Loukas YL. The significant role of amino acids during pregnancy: nutritional support. J Matern Fetal Neonatal Med 2020;33:334–40.10.1080/14767058.2018.1489795Search in Google Scholar PubMed
3. Manta-Vogli PD, Dotsikas Y, Loukas YL, Schulpis KH. The phenylketonuria patient: a recent dietetic therapeutic approach. Nutr Neurosci 2018:1–12. doi: 10.1080/1028415X.2018.1538196 (in press).Search in Google Scholar PubMed
4. Blachier F, Boutry C, Bos C, Tomé D. Metabolism and functions of L-glutamate in the epithelial cells of the small and large intestines. Am J Clin Nutr 2009;90:814S–21.10.3945/ajcn.2009.27462SSearch in Google Scholar PubMed
5. Wu G, Wu Z, Dai Z, Yang Y, Wang W, et al. Dietary requirements of “nutritionally non-essential amino acids” by animals and humans. Amino Acids 2013;44:1107–13.10.1007/s00726-012-1444-2Search in Google Scholar PubMed
6. Albrecht J, Sidoryk-Węgrzynowicz M, Zielińska M, Aschner M. Roles of glutamine in neurotransmission. Neuron Glia Biol 2010;6:263–76.10.1017/S1740925X11000093Search in Google Scholar PubMed
7. Brekke E, Morken TS, Sonnewald U. Glucose metabolism and astrocyte-neuron interactions in the neonatal brain. Neurochem Int 2015;82:33–41.10.1016/j.neuint.2015.02.002Search in Google Scholar PubMed
8. Glushakov AV, Glushakova O, Varshney M, Bajpai LK, Sumners C, et al. Long-term changes in glutamatergic synaptic transmission in phenylketonuria. Brain 2005;128(Pt 2):300–7.10.1093/brain/awh354Search in Google Scholar PubMed
9. Tsakiris S, Kouniniotou-Krontiri P, Schulpis KH, Stavridis JC. L-phenylalanine effect on rat brain acetylcholinesterase and Na+,K(+)-ATPase. Z Naturforsch C 1998;53:163–7.10.1515/znc-1998-3-404Search in Google Scholar PubMed
10. Doulgeraki A, Papadopoulou-Daifoti Z, Tsakiris S. Effects of L-phenylalanine on acetylcholinesterase and Na+,K+-ATPase activities in suckling rat frontal cortex, hippocampus and hypothalamus. Z Naturforsch C 2002;57:182–8.10.1515/znc-2002-1-230Search in Google Scholar PubMed
11. Tsakiris S, Angelogianni P, Schulpis KH, Stavridis JC. Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals. Clin Biochem 2000;33:103–6.10.1016/S0009-9120(99)00090-9Search in Google Scholar
12. Schulpis KH, Tjamouranis J, Karikas GA, Michelakakis H, Tsakiris S. In vivo effects of high phenylalanine blood levels on Na+,K+-ATPase, Mg2+-ATPase activities and biogenic amine concentrations in phenylketonuria. Clin Biochem 2002;35:281–5.10.1016/S0009-9120(02)00311-9Search in Google Scholar
13. Ng J, Papandreou A, Heales SJ, Kurian MA. Monoamine neurotransmitter disorders – clinical advances and future perspectives. Nat Rev Neurol 2015;11:567–84.10.1038/nrneurol.2015.172Search in Google Scholar PubMed
14. Zhang Z, Adelman AS, Rai D, Boettcher J, Lőnnerdal B. Amino acid profiles in term and preterm human milk through lactation: a systematic review. Nutrients 2013;5:4800–21.10.3390/nu5124800Search in Google Scholar PubMed PubMed Central
15. Li P, Knabe DA, Kim SW, Lynch CJ, Hutson SM, et al. Lactating porcine mammary tissue catabolizes branched-chain amino acids for glutamine and aspartate synthesis. J Nutr 2009;139:1502–9.10.3945/jn.109.105957Search in Google Scholar PubMed PubMed Central
16. Loukas YL, Soumelas GS, Dotsikas Y, Georgiou V, Molou E, et al. Expanded newborn screening in Greece: 30 months of experience. J Inherit Metab Dis 2010;33:S341–8.10.1007/s10545-010-9181-8Search in Google Scholar PubMed
17. Mandour I, El Gayar D, Amin M, Farid TM, Ali AA. Amino acid and acylcarnitine profiles in premature neonates: a pilot study. Ind J Pediatr 2013;80:736–44.10.1007/s12098-013-0980-4Search in Google Scholar PubMed
18. Oladipo OO, Weindel AL, Saunders AN, Dietzen DJ. Impact of premature birth and critical illness on neonatal range of plasma amino acid concentrations determined by LC-MS/MS. Mol Genet Metab 2011;104:476–9.10.1016/j.ymgme.2011.08.020Search in Google Scholar PubMed
19. Manta-Vogli PD, Schulpis KH, Loukas YL, Dotsikas Y. Quantitation of the arginine family amino acids in the blood of full-term infants perinatally in relation to their birth weight. J Pediatr Endocrinol Metab 2019;32:803–9.10.1515/jpem-2019-0146Search in Google Scholar PubMed
20. Dietzen DJ, Bennett MJ, Lo SF, Grey VL, Jones PM. Dried blood spot reference intervals for steroids and amino acids in a neonatal cohort of the national children’s study. Clin Chem 2016;62:1658–67.10.1373/clinchem.2016.263434Search in Google Scholar PubMed
21. ELSTAT: http://www.statistics.gr, access October 31st, 2019.Search in Google Scholar
22. Ivorra C, Garcia-Vicent C, Chaves FJ, Monleón D, Morales JM, et al. Metabolomic profiling in blood from umbilical cords of low birth weight newborns. J Transl Med 2012;10:142.10.1186/1479-5876-10-142Search in Google Scholar
23. Dearden L, Bouret SG, Ozanne SE. Sex and gender differences in developmental programming of metabolism. Mol Metab 2018;15:8–19.10.1016/j.molmet.2018.04.007Search in Google Scholar
24. Day PE, Ntani G, Crozier SR, Mahon PA, Inskip HM, et al. Maternal factors are associated with the expression of placental genes involved in amino acid metabolism and transport. PLoS One 2015;10:e0143653.10.1371/journal.pone.0143653Search in Google Scholar
25. Schulpis KH, Vlachos GD, Papakonstantinou ED, Karikas GA, Vlachos DG, et al. Maternal-neonatal amino acid blood levels in relation to the mode of delivery. Acta Obstet Gynecol Scand 2009;88:71–6.10.1080/00016340802578098Search in Google Scholar
26. Jain V, Singhal A. Catch up growth in low birth weight infants: striking a healthy balance. Rev Endocr Metab Disord 2012;13:141–7.10.1007/s11154-012-9216-6Search in Google Scholar
27. Yang L, Zhang Y, Yang J, Huang X. Effects of birth weight on profiles of dried blood amino-acids and acylcarnitines. Ann Clin Biochem 2018;55:92–9.10.1177/0004563216688038Search in Google Scholar
28. Fernstrom JD. Large neutral amino acids: dietary effects on brain neurochemistry and function. Amino Acids 2013;45: 419–30.10.1007/s00726-012-1330-ySearch in Google Scholar
29. Lemons JA, Reyman D, Moye L. Amino acid composition of preterm and term breastmilk during early lactation. Early Hum Dev 1983;8:323–9.10.1016/0378-3782(83)90015-4Search in Google Scholar
30. Yang Y, Yu B, Long W, Wang H, Wang Y, et al. Investigating the changes in amino acid values in premature infants: a pilot study. J Pediatr Endocrinol Metab 2018;31:435–41.10.1515/jpem-2017-0372Search in Google Scholar PubMed
©2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original Articles
- Vitamin D status in Israeli pediatric type 1 diabetes patients: the AWeSoMe Study Group experience and literature review
- Metabolic features and changes in glucose-induced serum glucagon-like peptide-1 levels in children with hypothalamic obesity
- Assessment of intima-media thickness of the carotid artery and intraluminal diameter of the brachial artery as cardiovascular risk markers in Brazilian adolescents with overweight or obesity
- The role of LCPUFA-ω3 on the obesity-associated hyperandrogenemia of pubertal girls: secondary analysis of a randomized clinical trial
- Genetic variants of the phenylalanine hydroxylase gene in patients with phenylketonuria in the northeast of Iran
- Oral health status of children with phenylketonuria
- Birth weight related blood concentrations of the neurotransmission amino acids glutamine plus glutamate, phenylalanine and tyrosine in full-term breastfed infants perinatally
- Hyperthyrotropinemia is common in preterm infants who are born small for gestational age
- Radioactive iodine therapy for pediatric Graves’ disease: a single-center experience over a 10-year period
- The decision-making levels of urine tetrasaccharide for the diagnosis of Pompe disease in the Turkish population
- A questionnaire study on sleep disturbances associated with Prader-Willi syndrome
- Body size measurements, digit ratio (2D:4D) and oestrogen and progesterone receptors’ expressions in juvenile gigantomastia
- The effect of celiac disease and gluten-free diet on pubertal development: a two-center study
- Observational study of disorders of sex development in Yaounde, Cameroon
- Letter to the Editor
- Reforming the male Tanner genital scale
- Short Communication
- The association between vitamin D levels and precocious puberty: a meta-analysis
- Case Reports
- Partial trisomy 9p and 14q microduplication in a patient with growth retardation: a case report and review of the literature
- Two different missense mutations of PEX genes in two similar patients with severe Zellweger syndrome: an argument on the genotype-phenotype correlation
- Patient report
- Marked phenotypic variable expression among brothers with duplication of Xq27.1 involving the SOX3 gene
- Case Report
- Does the risk of arterial hypertension increase in the course of triptorelin treatment?
Articles in the same Issue
- Frontmatter
- Original Articles
- Vitamin D status in Israeli pediatric type 1 diabetes patients: the AWeSoMe Study Group experience and literature review
- Metabolic features and changes in glucose-induced serum glucagon-like peptide-1 levels in children with hypothalamic obesity
- Assessment of intima-media thickness of the carotid artery and intraluminal diameter of the brachial artery as cardiovascular risk markers in Brazilian adolescents with overweight or obesity
- The role of LCPUFA-ω3 on the obesity-associated hyperandrogenemia of pubertal girls: secondary analysis of a randomized clinical trial
- Genetic variants of the phenylalanine hydroxylase gene in patients with phenylketonuria in the northeast of Iran
- Oral health status of children with phenylketonuria
- Birth weight related blood concentrations of the neurotransmission amino acids glutamine plus glutamate, phenylalanine and tyrosine in full-term breastfed infants perinatally
- Hyperthyrotropinemia is common in preterm infants who are born small for gestational age
- Radioactive iodine therapy for pediatric Graves’ disease: a single-center experience over a 10-year period
- The decision-making levels of urine tetrasaccharide for the diagnosis of Pompe disease in the Turkish population
- A questionnaire study on sleep disturbances associated with Prader-Willi syndrome
- Body size measurements, digit ratio (2D:4D) and oestrogen and progesterone receptors’ expressions in juvenile gigantomastia
- The effect of celiac disease and gluten-free diet on pubertal development: a two-center study
- Observational study of disorders of sex development in Yaounde, Cameroon
- Letter to the Editor
- Reforming the male Tanner genital scale
- Short Communication
- The association between vitamin D levels and precocious puberty: a meta-analysis
- Case Reports
- Partial trisomy 9p and 14q microduplication in a patient with growth retardation: a case report and review of the literature
- Two different missense mutations of PEX genes in two similar patients with severe Zellweger syndrome: an argument on the genotype-phenotype correlation
- Patient report
- Marked phenotypic variable expression among brothers with duplication of Xq27.1 involving the SOX3 gene
- Case Report
- Does the risk of arterial hypertension increase in the course of triptorelin treatment?
