Skip to main content
Article
Licensed
Unlicensed Requires Authentication

Association between UCP polymorphisms and adipokines with obesity in Mexican adolescents

  • , , , , , , and EMAIL logo
Published/Copyright: April 10, 2018
Journal of Pediatric Endocrinology and Metabolism
From the journal Journal of Pediatric Endocrinology and Metabolism Volume 31 Issue 5

Abstract

Background:

It has been reported that the uncoupling proteins (UCPs) can contribute to energy metabolism, and are thus involved in the pathogenesis of obesity. The objective of the study was to analyze the association between UCP polymorphisms, clinical parameters and leptin and adiponectin plasma levels in an adolescent population with overweight and obesity.

Methods:

We analyzed the UCP1 -3826 C/T, UCP2-866 G/A, Ala55Val and UCP3 -55 C/T polymorphisms and the levels of adipokines in adolescents with normal weight and with overweight or obesity. The study included 270 students aged between 12 and 18 years categorized according to the percentiles from Mexico City. Adipokines levels were measured by immunoassay methods and the UCP polymorphisms were determined using Taqman real-time polymerase chain reaction (RT-PCR).

Results:

No significant differences were found in the UCP polymorphisms in seven inheritance models studied. Most of the significant differences in the clinical parameters were found under a recessive model, the UCP2 -866 polymorphism was associated with diastolic blood pressure (p=0.008), triglycerides (p=0.045), low-density lipoprotein-cholesterol (LDL-C) (p=0.003), high-density lipoprotein-cholesterol (HDL-C) (p=0.050) and plasma levels of leptin (p<0.001). Also, the obese group was found to have higher leptin levels and lower adiponectin levels in GA+AA vs. GG (recessive model).

Conclusions:

This study demonstrated a direct relationship between the clinical characteristics and UCP2-866 in a recessive model, associated with high levels of leptin and decreased levels of adiponectin in an obese or overweight Mexican adolescent population.

Keywords: adipokines; genetic polymorphisms; Mexican population; obesity; uncoupling proteins

Acknowledgments

The authors thank the participants of this study.

  1. Author contributions: R. Sámano, R. Gamboa and designed the study; R. Sámano, C. Huesca-Gómez, R. López-Marure A. Rodríguez-Ventura, AK. Hernandez-Cabrera and M. Tolentino collected the data; R. Sámano, R. Gamboa, C. Huesca-Gómez and A. Rodríguez-Ventura contributed to data analysis and interpretation; R. Gamboa drafted the manuscript. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: Funding for this study was provided by Instituto Nacional de Perinatología in the public sector, Funder Id: 10.13039/501100007686, registry 212250-49541 and Consejo Nacional de Ciencia y Tecnología, project number 179967 CB2012-01.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. Eckel RH, Cornier MA. Update on the NCEP ATP-III emerging cardiometabolic risk factors. BMC Med 2014;26:115.10.1186/1741-7015-12-115Search in Google Scholar

2. Arnlöv J, Ingelsson E, Sundström J, Lind L. Impact of body mass index and the metabolic syndrome on the risk of cardiovascular disease and death in middle-aged men. Circulation 2010;121:230–6.10.1161/CIRCULATIONAHA.109.887521Search in Google Scholar

3. Oberkofler H, Liu YM, Esterbauer H, Hell E, Krempler F, et al. Uncoupling protein-2 gene: reduced mRNA expression in intraperitoneal adipose tissue of obese humans. Diabetologia 1998;41:940–6.10.1007/s001250051011Search in Google Scholar

4. Pinkney JH, Boss O, Bray GA, Bulmer K, Coppack SW, et al. Physiological relationships of uncoupling protein-2 gene expression in human adipose tissue in vivo. J Clin Endocrinol Metab 2000;85:2312–7.10.1210/jc.85.6.2312Search in Google Scholar

5. Argilés JM, Busquets S, López-Soriano FJ. The role of uncoupling proteins in pathophysiological states. Biochem Biophys Res Commun 2002;293:1145–52.10.1016/S0006-291X(02)00355-8Search in Google Scholar

6. Schrauwen P, Hesselink MK, Vaartjes I, Kornips E, Saris WH, et al. Effect of acute exercise on uncoupling protein 3 is a fat metabolism-mediated effect. Am J Physiol Endocrinol Metab 2002;282:E11–7.10.1152/ajpendo.2002.282.1.E11Search in Google Scholar PubMed

7. Jun HS, Kim IK, Lee HJ, Lee HJ, Kang JH, et al. Effects of UCP2 and UCP3 variants on the manifestation of overweight in Korean children. Obesity 2009;17:355–62.10.1038/oby.2008.531Search in Google Scholar PubMed

8. León-Mimila P, Villamil-Ramírez H, Villalobos-Comparán M, Villarreal-Molina T, Romero-Hidalgo S, et al. Contribution of common genetic variants to obesity and obesity-related traits in Mexican children and adults. PLoS One 2013;8:e70640.10.1371/journal.pone.0070640Search in Google Scholar PubMed PubMed Central

9. Mexitalia M, Yamauchi T, Utari A, Sjarif DR, Subagio HW, et al. The role of uncoupling protein 2 and 3 genes polymorphism and energy expenditure in obese Indonesian children. J Pediatr Endocrinol Metab 2013;26:441–7.10.1515/jpem-2012-0311Search in Google Scholar PubMed

10. Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, et al. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1. Biochem Biophys Res Commun 2012;425:556–9.10.1016/j.bbrc.2012.08.023Search in Google Scholar PubMed

11. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab 2001;86:1930–5.10.1210/jcem.86.5.7463Search in Google Scholar

12. Kubota N, Yano W, Kubota T, Yamauchi T, Itoh S, et al. Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake. Cell Metab 2007;6:55–68.10.1016/j.cmet.2007.06.003Search in Google Scholar

13. Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, et al. Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol 2000;20:1595–9.10.1161/01.ATV.20.6.1595Search in Google Scholar

14. Lafontan M, Viguerie N. Role of adipokines in the control of energy metabolism: focus on adiponectin. Curr Opin Pharmacol 2006;6:580–5.10.1016/j.coph.2006.08.002Search in Google Scholar

15. Chevillotte E, Giralt M, Miroux B, Ricquier D, Villarroya F. Uncoupling protein-2 controls adiponectin gene expression in adipose tissue through the modulation of reactive oxygen species production. Diabetes 2007;56:1042–50.10.2337/db06-1300Search in Google Scholar

16. Taghadomi Masoumi Z, Eshraghian MR, Hedayati M, Pishva H. Association between uncoupling protein 2, adiponectin and resting energy expenditure in obese women with normal and low resting energy expenditure. Gynecol Endocrinol 2018;34:166–70.10.1080/09513590.2017.1379492Search in Google Scholar

17. Menzaghi C, Trischitta V, Doria A. Genetic influences of adiponectin on insulin resistance, type 2 diabetes, and cardiovascular disease. Diabetes 2007;56:1198–209.10.2337/db06-0506Search in Google Scholar

18. Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Nature 1998;395:763–70.10.1038/27376Search in Google Scholar

19. Spiegelman BM, Flier JS. Obesity and the regulation of energy balance. Cell 2001;104:531–43.10.1016/S0092-8674(01)00240-9Search in Google Scholar

20. Esler M, Vaz M, Collier G, Nestel P, Jennings G, et al. Leptin in human plasma is derived in part from the brain, and cleared by the kidneys. Lancet 1998;351:879.10.1016/S0140-6736(05)70289-0Search in Google Scholar

21. Maratos-Flier E. The long reach of leptin. Nat Med 2008;14:604–6.10.1038/nm0608-604Search in Google Scholar

22. Coppola A, Liu ZW, Andrews ZB, Paradis E, Roy MC, et al. A central thermogenic-like mechanism in feeding regulation: an interplay between arcuate nucleus T3 and UCP2. Cell Metab 2007;5:21–33.10.1016/j.cmet.2006.12.002Search in Google Scholar

23. López M, Varela L, Vázquez MJ, Rodríguez-Cuenca S, González CR, et al. Hypothalamic AMPK and fatty acid metabolism mediate thyroid regulation of energy balance. Nat Med 2010;16:1001–8.10.1038/nm.2207Search in Google Scholar

24. Gonzalez-Yanes C, Sánchez-Margalet V. Pancreastatin, a chromogranin A-derived peptide, inhibits leptin and enhances UCP-2 expression in isolated rat adipocytes. Cell Mol Life Sci 2003;60:2749–56.10.1007/s00018-003-3346-7Search in Google Scholar

25. Zhou Y, Rui L. Leptin signaling and leptin resistance. Front Med 2013;7:207–22.10.1007/s11684-013-0263-5Search in Google Scholar

26. Labruna G, Pasanisi F, Nardelli C, Tarantino G, Vitale DF, et al. UCP1 -3826 AG+GG genotypes, adiponectin, and leptin/adiponectin ratio in severe obesity. Endocrinol Invest 2009;32:525–9.10.1007/BF03346500Search in Google Scholar

27. Lim KI, Shin YA. Impact of UCP2 polymorphism on long-term exercise-mediated changes in adipocytokines and markers of metabolic syndrome. Aging Clin Exp Res 2014;26:491–6.10.1007/s40520-014-0213-3Search in Google Scholar

28. Zurbano R, Ochoa MC, Moreno-Aliaga MJ, Martínez JA, Martí A. Grupo de Estudio Navarro de la obesidad infantil. Influence of the -866/A polymorphism of the UCP2 gene on an obese. Nutr Hosp 2006;21:52–6.Search in Google Scholar

29. Lohman TG, Roche AF, Martorell R. Anthropometric standardization reference manual. Champaign, Ill, USA: Human Kinetics Books, 1988.Search in Google Scholar

30. WHO Multicentre Growth Reference Study Group. WHO Child Growth Standards: length/height-for-age, weight-for-age, weight-for-length, weight-for-height and body mass index-for-age: methods and development. Geneva: World Health Organization, 2007.Search in Google Scholar

31. Franco-Hincapié L, Duque CE, Parra MV, Gallego N, Villegas A, et al. Association between polymorphisms in uncoupling proteins and type 2 diabetes in a northwestern Colombian population. Biomedica 2009;29:108–18.10.7705/biomedica.v29i1.46Search in Google Scholar

32. Bray MS, Boerwinkle E, Hanis CL. Linkage analysis of candidate obesity genes among the Mexican-American population of Starr County, Texas. Genet Epidemiol 1999;16:397–411.10.1002/(SICI)1098-2272(1999)16:4<397::AID-GEPI6>3.0.CO;2-XSearch in Google Scholar

33. Walder K, Norman RA, Hanson RL, Schrauwen P, Neverova M, et al. Association between uncoupling protein polymorphisms (UCP2-UCP3) and energy metabolism/obesity in Pima indians. Hum Mol Genet 1998;7:1431–5.10.1093/hmg/7.9.1431Search in Google Scholar

34. Esterbauer H, Schneitler C, Oberkofler H, Ebenbichler C, Paulweber B, et al. A common polymorphism in the promoter of UCP2 is associated with decreased risk of obesity in middle-aged humans. Nat Genet 2001;28:178–83.10.1038/88911Search in Google Scholar PubMed

35. Brondani LA, Assman TS, De Souza BM, Boucas AP, Canani LH, et al. Meta-analysis reveals the association of common variants in the uncoupling protein (UCP)1-3 genes with body mass index variability. PLoS One 2014;9:e96411.10.1371/journal.pone.0096411Search in Google Scholar PubMed PubMed Central

36. Mancini FP, Sabatino L, Colantuoni V, Pasanisi F, Finelli C, et al. Variants of uncoupling protein-2 gene and obesity: interaction with peroxisome proliferator-activated receptor gamma 2. Clin Endocrinol 2003;59:817–22.10.1046/j.1365-2265.2003.01926.xSearch in Google Scholar PubMed

37. Mottagui-Tabar S, Hoffstedt J, Brookes AJ, Jiao H, Arner P, et al. Association of ADRB1 and UCP3 gene polymorphisms with insulin sensitivity but not obesity. Horm Res 2008;69:31–6.10.1159/000111793Search in Google Scholar PubMed

38. Krauss S, Zhang CY, Scorrano L, Dalgaard LT, St-Pierre J, et al. Superoxide-mediated activation of uncoupling protein 2 causes pancreatic β cell dysfunction. J Clin Invest 2003;112:1831–42.10.1172/JCI200319774Search in Google Scholar

39. Liu J, Li J, Li WJ, Wang CM. The role of uncoupling proteins in diabetes mellitus. J Diabetes Res 2013;2013:585897.10.1155/2013/585897Search in Google Scholar PubMed PubMed Central

40. Lee HJ, Ryu HJ, Shin HD, Park BL, Kim JY, et al. Associations between polymorphisms in the mitochondrial uncoupling proteins (UCPs) with T2DM. Clin Chim Acta 2008;398:27–33.10.1016/j.cca.2008.07.029Search in Google Scholar PubMed

41. Dhamrait SS, Stephens JW, Cooper JA, Acharya J, Mani AR, et al. FT Cardiovascular risk in healthy men and markers of oxidative stress in diabetic men are associated with common variation in the gene for uncoupling protein 2. Eur Heart J 2004;25:468–75.10.1016/j.ehj.2004.01.007Search in Google Scholar PubMed

42. Kring SI, Larsen LH, Holst C, Toubro S, Hansen T, et al. Genotype-phenotype associations in obesity dependent on definition of the obesity phenotype. Obesity Facts 2008;1:138–45.10.1159/000137665Search in Google Scholar PubMed PubMed Central

43. Salopuro T, Pulkkinen L, Lindström J, Kolehmainen M, Tolppanen AM, et al. Variation in the UCP2 and UCP3 genes associates with abdominal obesity and serum lipids: the Finnish Diabetes Prevention Study. BMC Med Genet 2009;10:94.10.1186/1471-2350-10-94Search in Google Scholar PubMed PubMed Central

Received: 2017-7-4
Accepted: 2018-2-5
Published Online: 2018-4-10
Published in Print: 2018-5-24

©2018 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Review
  3. Review and evaluation of patient-centered psychosocial assessments for children with central precocious puberty or early puberty
  4. Mini Review
  5. Childhood obesity: how long should we wait to predict weight?
  6. Original Articles
  7. Glycated hemoglobin A1c as a screening test for detecting type 2 diabetes mellitus in obese children and adolescents
  8. Effect of a multidisciplinary treatment program on eating behavior in overweight and obese preschool children
  9. Cord blood klotho levels are inversely associated with leptin in healthy Latino neonates at risk for obesity
  10. Still too little, too late? Ten years of growth hormone therapy baseline data from the NordiNet® International Outcome Study
  11. Body composition and metabolic health of young male adults with childhood-onset multiple pituitary hormone deficiency after cessation of growth hormone treatment
  12. Hematuria as an adverse outcome following provocative growth hormone stimulation testing in children
  13. Bone mineral density comparison of adolescents with constitutional thinness and anorexia nervosa
  14. Adult height in patients with familial male-limited precocious puberty and the role of an aromatase inhibitor in patient management
  15. Association between UCP polymorphisms and adipokines with obesity in Mexican adolescents
  16. Impact of childhood type 1 diabetes on maternal work-family relations
  17. Case Reports
  18. Hyperthyroidism in an infant of a mother with autoimmune hypothyroidism with positive TSH receptor antibodies
  19. Sanjad-Sakati syndrome with macrocytic anemia and failure to thrive: a case from South Jordan
  20. In iodine-induced thyrotoxicosis, steroid therapy today could keep the surgical knife at bay
  21. Graves’ disease following allogenic hematopoietic stem cell transplantation for severe aplastic anemia: case report and literature review
https://doi.org/10.1515/jpem-2017-0262
Keywords for this article
adipokines; genetic polymorphisms; Mexican population; obesity; uncoupling proteins

Articles in the same Issue

  1. Frontmatter
  2. Review
  3. Review and evaluation of patient-centered psychosocial assessments for children with central precocious puberty or early puberty
  4. Mini Review
  5. Childhood obesity: how long should we wait to predict weight?
  6. Original Articles
  7. Glycated hemoglobin A1c as a screening test for detecting type 2 diabetes mellitus in obese children and adolescents
  8. Effect of a multidisciplinary treatment program on eating behavior in overweight and obese preschool children
  9. Cord blood klotho levels are inversely associated with leptin in healthy Latino neonates at risk for obesity
  10. Still too little, too late? Ten years of growth hormone therapy baseline data from the NordiNet® International Outcome Study
  11. Body composition and metabolic health of young male adults with childhood-onset multiple pituitary hormone deficiency after cessation of growth hormone treatment
  12. Hematuria as an adverse outcome following provocative growth hormone stimulation testing in children
  13. Bone mineral density comparison of adolescents with constitutional thinness and anorexia nervosa
  14. Adult height in patients with familial male-limited precocious puberty and the role of an aromatase inhibitor in patient management
  15. Association between UCP polymorphisms and adipokines with obesity in Mexican adolescents
  16. Impact of childhood type 1 diabetes on maternal work-family relations
  17. Case Reports
  18. Hyperthyroidism in an infant of a mother with autoimmune hypothyroidism with positive TSH receptor antibodies
  19. Sanjad-Sakati syndrome with macrocytic anemia and failure to thrive: a case from South Jordan
  20. In iodine-induced thyrotoxicosis, steroid therapy today could keep the surgical knife at bay
  21. Graves’ disease following allogenic hematopoietic stem cell transplantation for severe aplastic anemia: case report and literature review
Downloaded on 19.4.2026 from https://www.degruyterbrill.com/document/doi/10.1515/jpem-2017-0262/html
Scroll to top button