Home Evaluation of gastric tolerability for long-term use of diclofenac and celecoxib in male albino rats and potential gastroprotective benefits of royal jelly: a randomized controlled trial
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Evaluation of gastric tolerability for long-term use of diclofenac and celecoxib in male albino rats and potential gastroprotective benefits of royal jelly: a randomized controlled trial

  • Amira A.A. Othman ORCID logo EMAIL logo
Published/Copyright: December 17, 2024
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 22 Issue 1

Abstract

Objectives

Nonsteroidal anti-inflammatory drugs (NSAIDs) are used for pain and inflammation relief. Our study aimed to explore the ulcerogenic effect of long-term diclofenac and celecoxib administration on male albino stomachs, focusing on the possible gastroprotective effect of royal jelly (RJ) administration.

Methods

Five equal groups of 50 male albino rats. The drug dosages were: diclofenac potassium (10 mg/kg/day), celecoxib (50 mg/kg/day), and RJ (300 mg/kg/day), for 4 weeks. Group 1 received no medication. Group 2 received oral diclofenac potassium. Group 3 received oral RJ plus diclofenac potassium. Group 4 received celecoxib orally. Group 4 received oral RJ plus celecoxib. When the experiment was over, rats were euthanized, blood samples were gathered, and stomachs were dissected out. Stomachs were examined for ulcer counts. Serum levels of MDA and SOD were determined. Gastric mucosa contents of MDA, SOD, PGE2, MPO, apoptotic (Bax), and anti-apoptotic (Bcl-2) genes were measured. Gastric tissue was also analyzed histopathologically.

Results

Long-term administration of diclofenac and celecoxib, in such dose and duration, caused each of the aforementioned parameters to significantly deteriorate, with significant improvement with RJ co-administration. Diclofenac developed severe gastric ulcers in group 2, and RJ co-administration significantly reduced the gastric mucosa damage in group 3. Celecoxib developed no gastric ulcer in both groups 4 and 5.

Conclusions

Long-term use of diclofenac in male albino rats caused severe gastric ulcers with significant gastroprotective effects of RJ. Celecoxib provides preferable GI tolerability; thus, it should be prescribed for patients at increased risk of gastrointestinal bleeding requiring NSAIDs.

Keywords: celecoxib; diclofenac; gastric ulcer; royal jelly
Corresponding author: Amira A.A. Othman, Department of Internal Medicine, Faculty of Medicine, Suez University, Suez, Egypt, E-mail:

  1. Research ethics: Ethical approval for this study was sought from and granted under a protocol approved by the local animal ethics committee and was in accordance with the recommendations of the European Union, under the Prevention of Cruelty to Animals Legislation, (UK) 1986.

  2. Informed consent: Not applicable.

  3. Author contributions: A.O.: concept and design of the study, interpreted the results, analyzed the data, drafted the manuscript, critically revised the manuscript, and approved the final version to be published.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The author states no conflict of interest.

  6. Research funding: None declared.

  7. Data availability: All relevant data are included in this published article.

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Received: 2024-09-18
Accepted: 2024-11-19
Published Online: 2024-12-17

© 2024 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Terpene-based novel invasomes: pioneering cancer treatment strategies in traditional medicine
  4. Mind-body practices for people living with dementia and their family carers: a systematic review
  5. Methodological advances in formulation and assay of herbal resources-based topical drug delivery systems
  6. Research Articles
  7. A potential therapeutic role of resveratrol in mitigating hepatotoxicity induced by paracetamol and alcohol
  8. A preclinical study on effect of betanin on sodium fluoride induced hepatorenal toxicity in Wistar rats
  9. Phytochemical characterization and evaluation of the biological activity spectrum of ethanolic fruit extract of Garcinia indica: a less explored plant of Ayurveda
  10. Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts
  11. Albizia ferruginea (Guill. & Perr.) Benth. leaf abates deregulation of P53, IRS, HsD17β2, FTO, and CYP11a genes in polycystic ovarian syndrome rat
  12. Vernonia amygdalina aqueous leaf extract modulates metformin pharmacokinetics, inhibits CYP3A4 and CYP2C9 enzymes in streptozotocin-induced diabetic rats
  13. Quantitative determination of the antibacterial activity of licorice (Glycyrrhiza glabra) and tetracycline gel against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) – a microbiological in vitro study
  14. In-silico screening of bioactive compounds of Moringa oleifera as potential inhibitors targeting HIF-1α/VEGF/GLUT-1 pathway against breast cancer
  15. Isolation, structural characterization, and molecular docking studies on the bioactive compound from n-Hexane extract of Emilia sonchifolia (L.) DC against the pancreatic cancer target Aurora 2 Kinase
  16. The impact of Omega-3 supplementation on arrhythmia reduction in acute coronary syndrome patients: a randomized clinical trial
  17. Evaluation of gastric tolerability for long-term use of diclofenac and celecoxib in male albino rats and potential gastroprotective benefits of royal jelly: a randomized controlled trial
  18. The efficacy of thread embedding acupuncture on pain score, neck disability index, and pressure pain threshold for myofascial pain therapy in the upper trapezius muscle
  19. Correction of hypoxic effects on target organs in pneumonia with phytotherapy
https://doi.org/10.1515/jcim-2024-0324
Keywords for this article
celecoxib; diclofenac; gastric ulcer; royal jelly

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Terpene-based novel invasomes: pioneering cancer treatment strategies in traditional medicine
  4. Mind-body practices for people living with dementia and their family carers: a systematic review
  5. Methodological advances in formulation and assay of herbal resources-based topical drug delivery systems
  6. Research Articles
  7. A potential therapeutic role of resveratrol in mitigating hepatotoxicity induced by paracetamol and alcohol
  8. A preclinical study on effect of betanin on sodium fluoride induced hepatorenal toxicity in Wistar rats
  9. Phytochemical characterization and evaluation of the biological activity spectrum of ethanolic fruit extract of Garcinia indica: a less explored plant of Ayurveda
  10. Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts
  11. Albizia ferruginea (Guill. & Perr.) Benth. leaf abates deregulation of P53, IRS, HsD17β2, FTO, and CYP11a genes in polycystic ovarian syndrome rat
  12. Vernonia amygdalina aqueous leaf extract modulates metformin pharmacokinetics, inhibits CYP3A4 and CYP2C9 enzymes in streptozotocin-induced diabetic rats
  13. Quantitative determination of the antibacterial activity of licorice (Glycyrrhiza glabra) and tetracycline gel against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) – a microbiological in vitro study
  14. In-silico screening of bioactive compounds of Moringa oleifera as potential inhibitors targeting HIF-1α/VEGF/GLUT-1 pathway against breast cancer
  15. Isolation, structural characterization, and molecular docking studies on the bioactive compound from n-Hexane extract of Emilia sonchifolia (L.) DC against the pancreatic cancer target Aurora 2 Kinase
  16. The impact of Omega-3 supplementation on arrhythmia reduction in acute coronary syndrome patients: a randomized clinical trial
  17. Evaluation of gastric tolerability for long-term use of diclofenac and celecoxib in male albino rats and potential gastroprotective benefits of royal jelly: a randomized controlled trial
  18. The efficacy of thread embedding acupuncture on pain score, neck disability index, and pressure pain threshold for myofascial pain therapy in the upper trapezius muscle
  19. Correction of hypoxic effects on target organs in pneumonia with phytotherapy
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