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Albizia ferruginea (Guill. & Perr.) Benth. leaf abates deregulation of P53, IRS, HsD17β2, FTO, and CYP11a genes in polycystic ovarian syndrome rat

  • Akingbolabo Daniel Ogunlakin EMAIL logo , Meek Oyinlola Meruwoma , Princewill Obinna Ihiasota , Oluwafemi Adeleke Ojo ORCID logo , Adeyemi Abdullahi Adegoke , Idayat Adeola Akinwumi , Owoola Azeezat Ambali , Oyindamola Esther Awosola and Mubo Adeola Sonibare
Published/Copyright: November 29, 2024
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 22 Issue 1

Abstract

Objectives

This study investigated the potential ameliorative effects of Albizia ferruginea leaves on letrozole-induced polycystic ovarian syndrome (PCOS) in Wistar rats.

Methods

PCOS was induced in 25 female Wistar rats by administering letrozole (1 mg/kg), followed by treatment with 100 and 250 mg/kg body weight A. ferruginea leaf methanolic extract, as well as 1 mg/kg body weight of clomiphene citrate as standard.

Results

An acute toxicity study revealed a toxic dosage of 2,000 mg/kg for the plant extract. The A. ferruginea extract exhibited potent hydroxyl radical scavenging ability. Treatment with A. ferruginea leaf extract improved the irregular estrus cycle and hormonal imbalance. Additionally, the extract administration led to decreased testosterone and increased estradiol levels when compared to the untreated PCOS rat. Furthermore, methanol extract normalizes the levels of insulin receptor substrate (IRS), type 2 17-HSD (HsD17β2), P53, 11a-hydroxylase/17,20-desmolase (CYP11a), and fat mass and obesity-associated (FTO), genes in the cervix of PCOS rats.

Conclusions

Overall, A. ferruginea demonstrated beneficial properties on polycystic ovary circumstances in rats, presenting its potential as a promising treatment for PCOS.

Keywords: polycystic ovarian syndrome; Albizia ferruginea ; antioxidant; in vivo assay; gene expression
Corresponding author: Akingbolabo Daniel Ogunlakin, Good Health and Wellbeing Research Cluster, Bowen University, Iwo 232101, Nigeria; and Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Laboratory (PMTCB-RL), Department of Biochemistry, Bowen University, Iwo 232101, Nigeria, E-mail:

Acknowledgments

We appreciate Bowen University for supporting this research.

  1. Research ethics: Every relevant institutional, governmental, and/or international law pertaining to the use and care of animals was adhered to. The research was also approved by the University of Ibadan Animal Care and Use Research Ethics Committee (UI-ACUREC/19/0051).

  2. Informed consent: Not applicable.

  3. Author contributions: Conceptualization: ADO; formal Analysis: all author; software: ADO, MOM and POI; funding acquisition: ADO, OAO, AAA and OEA; writing (original draft): ADO, MOM and POI; writing (review & editing): ADO; supervision: ADO.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The authors state no conflict of interest.

  6. Research funding: None declared.

  7. Data availability: Data will be made available on request.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/jcim-2024-0287).

Received: 2024-08-27
Accepted: 2024-11-07
Published Online: 2024-11-29

© 2024 Walter de Gruyter GmbH, Berlin/Boston

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  5. Methodological advances in formulation and assay of herbal resources-based topical drug delivery systems
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  9. Phytochemical characterization and evaluation of the biological activity spectrum of ethanolic fruit extract of Garcinia indica: a less explored plant of Ayurveda
  10. Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts
  11. Albizia ferruginea (Guill. & Perr.) Benth. leaf abates deregulation of P53, IRS, HsD17β2, FTO, and CYP11a genes in polycystic ovarian syndrome rat
  12. Vernonia amygdalina aqueous leaf extract modulates metformin pharmacokinetics, inhibits CYP3A4 and CYP2C9 enzymes in streptozotocin-induced diabetic rats
  13. Quantitative determination of the antibacterial activity of licorice (Glycyrrhiza glabra) and tetracycline gel against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) – a microbiological in vitro study
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https://doi.org/10.1515/jcim-2024-0287
Keywords for this article
polycystic ovarian syndrome; Albizia ferruginea; antioxidant; in vivo assay; gene expression

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Terpene-based novel invasomes: pioneering cancer treatment strategies in traditional medicine
  4. Mind-body practices for people living with dementia and their family carers: a systematic review
  5. Methodological advances in formulation and assay of herbal resources-based topical drug delivery systems
  6. Research Articles
  7. A potential therapeutic role of resveratrol in mitigating hepatotoxicity induced by paracetamol and alcohol
  8. A preclinical study on effect of betanin on sodium fluoride induced hepatorenal toxicity in Wistar rats
  9. Phytochemical characterization and evaluation of the biological activity spectrum of ethanolic fruit extract of Garcinia indica: a less explored plant of Ayurveda
  10. Scientific investigation on antibacterial, antioxidant, cytotoxic effects and TLC bioautography of Terminalia schimperiania stem bark extracts
  11. Albizia ferruginea (Guill. & Perr.) Benth. leaf abates deregulation of P53, IRS, HsD17β2, FTO, and CYP11a genes in polycystic ovarian syndrome rat
  12. Vernonia amygdalina aqueous leaf extract modulates metformin pharmacokinetics, inhibits CYP3A4 and CYP2C9 enzymes in streptozotocin-induced diabetic rats
  13. Quantitative determination of the antibacterial activity of licorice (Glycyrrhiza glabra) and tetracycline gel against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi) – a microbiological in vitro study
  14. In-silico screening of bioactive compounds of Moringa oleifera as potential inhibitors targeting HIF-1α/VEGF/GLUT-1 pathway against breast cancer
  15. Isolation, structural characterization, and molecular docking studies on the bioactive compound from n-Hexane extract of Emilia sonchifolia (L.) DC against the pancreatic cancer target Aurora 2 Kinase
  16. The impact of Omega-3 supplementation on arrhythmia reduction in acute coronary syndrome patients: a randomized clinical trial
  17. Evaluation of gastric tolerability for long-term use of diclofenac and celecoxib in male albino rats and potential gastroprotective benefits of royal jelly: a randomized controlled trial
  18. The efficacy of thread embedding acupuncture on pain score, neck disability index, and pressure pain threshold for myofascial pain therapy in the upper trapezius muscle
  19. Correction of hypoxic effects on target organs in pneumonia with phytotherapy
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