Crude extract from Euphorbia prostrata extended curative period of glibenclemide in alloxan-induced diabetic rats

Mohammad Parvez; Farrukh Hussain; Murad Khan

doi:10.1515/jcim-2022-0315

Article

Crude extract from Euphorbia prostrata extended curative period of glibenclemide in alloxan-induced diabetic rats

Mohammad Parvez , Farrukh Hussain and Murad Khan

Published/Copyright: January 20, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 2

Abstract

Objectives

Euphorbia prostrata is traditionally used alongside antidiabetic agents to manage diabetes. Bioactive ingredients of medicinal herbs may alter the overall pharmacokinetics of antidiabetic agents.

Methods

We assessed hypoglycemic activities of ethanolic plant extract (EPE) singly and its effects on antidiabetic properties of gliclazide, glibenclemide and metformin in allaxonized rats. Varying concentrations of EPE (250 and 500 mg/kg) with or without metformin (10 mg/kg), glibenclemide (2 mg/kg) and gliclazide (5 mg/kg) were orally administered to evaluate herb-drug interaction.

Results

The levels of blood glucose declined significantly after treatment with metformin, glibenclemide and gliclazide singly (p<0.01) or concomitantly with EPE (p<0.001). Concentration dependent mild to moderate reduction (5.2 and 10.0%) was registered in blood glucose for 250 and 500 mg/kg of EPE respectively. The overall reduction in blood glucose due to combined treatment with EPE and standard agents was additive. On the other hand, synergistic herb-drug interaction was registered for insulin levels in rats that received glibenclamide and gliclazide alongside EPE. Extract with metformin had antagonistic insulin outcome. Regarding the duration of hypoglycemic activities, periodical changes were similar in case of glibenclamide and gliclazide separately or in combination with EPE. However, in case of metformin with extract, the blood glucose continued to decline for 14 h and retained at 15.0% below the baseline values even after 24 h of treatment.

Conclusions

In conclusion, the extract itself had weak hypoglycemic effects but prolonged the therapeutic duration of metformin to more than 24 h when administered combinedly.

Keywords: Euphorbia prostrata ; glibenclamide; gliclazide; herb-drug interaction; medicinal herbs; metformin

Corresponding author: Mohammad Parvez, Institute of Biological Sciences, Sarhad University of Science and Information Technology (SUIT), Peshawar, Pakistan, Phone: +92-3349300239, E-mail: mparvezswati@yahoo.com

Acknowledgments

The laboratory facilities provided by Sarhad University of Science and Technology, Pakistan are acknowledged.

Research funding: None declared.
Author contribution: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: The study was conducted after approval from Ethical Committee, Department of Pharmacy (Reference number 12/EC-14/Pharm) University of Peshawar.

References

1. Solikhah, TI, Setiawan, B, Ismukada, DR. Antidiabetic activity of papaya leaf extract (Carica papaya L.) isolated with maceration method in alloxan-induces diabetic mice. Sys Rev Pharm 2020;11:774–8. https://doi.org/10.31838/srp.2020.9.109.Search in Google Scholar

2. Mia, S, Roy, R, Akter, A, Barek, MA, Basher, MA. Antidiabetic and antihyperlipidemic activities of the leaf latex extract of Aloe megalacantha Baker (Aloaceae) in streptozotocin-induced diabetic model. Clinical Phytoscience 2019;5:26. https://doi.org/10.1186/s40816-019-0119-x.Search in Google Scholar

3. Karigidi, KO, Olaiya, CO. Antidiabetic activity of corn steep liquor extract of Curculigo pilosa and its solvent fractions in streptozotocin-induced diabetic rats. J Tradit Complement Med 2019;10:555–64. https://doi.org/10.1016/j.jtcme.2019.06.005.Search in Google Scholar PubMed PubMed Central

4. AL-Ishaq, RK, Abotaleb, M, Kubatka, P, Kajo, K, Busselberg, D. Flavonoids and their anti-diabetic effects: cellular mechanisms and effects to improve blood sugar levels. Biomolecules 2019;9:430–65. https://doi.org/10.3390/biom9090430.Search in Google Scholar PubMed PubMed Central

5. Peng, Y, Gao, Y, Zhang, X, Zhang, C, Wang, X, Zhang, H, et al.. Antidiabetic and hepatoprotective activity of the roots of Calanthe mbriata Franch. Biomed Pharmacother 2019;111:60–7. https://doi.org/10.1016/j.biopha.2018.12.066.Search in Google Scholar PubMed

6. Yetendje, LC, Njateng, GSS, Dongmo, AJA, Mouokeu, RS, Feudjio, C, Tamekou, SL, et al.. In vivo antidiabetic activity and mechanism of action of three Cameroonian medicinal plant extracts. Int J Res Granthaalayah 2019;7:415–30. https://doi.org/10.5281/zenodo.3401409.Search in Google Scholar

7. Gani, I, Maqbool, M, Dar, MA. Anti-diabetic effects of some medicinal plants in experimental animals: a review. Asian J Pharmaceut Res Dev 2019;7:66–9. https://doi.org/10.22270/ajprd.v7i1.469.Search in Google Scholar

8. Njangiru, IK, Gitimu, MR, Njagi, ENM. In vivo antidiabetic activity of aqueous extract of Psidium Quajava in alloxanised diabetic mice. J Med Biomed Appl Sci 2017;5:1–6. https://doi.org/10.15520/.V5I1.42.Search in Google Scholar

9. Shah, MAR, Khan, RA, Ahmed, M. Anti-diabetic activity of Iphiona aucheri leaf extract. Bangladesh J Pharmacol 2020;15:99–109. https://doi.org/10.3329/bjp.v15i4.44170.Search in Google Scholar

10. Rombola, L, Scuteri, D, Marilisa, S, Watanabe, C, Morrone, LA, Bagetta, G, et al.. Pharmacokinetic interactions between herbal medicines and drugs: their mechanisms and clinical relevance. Life 2020;10:106. https://doi.org/10.3390/life10070106.Search in Google Scholar PubMed PubMed Central

11. Melaku, BC, Getnet, G. Evaluation of antidiabetic and antioxidant potential of hydromethanolic seed extract of Datura stramonium in mice. J Clin Exp Pharmacol 2020;10:267–73. https://doi.org/10.2147/JEP.S258522.Search in Google Scholar PubMed PubMed Central

12. Subaraman, M, Gayathri, R, Priya, VV. In vitro antidiabetic activity of crude acetone leaf extract of Annona muricata. Drug Invent Today 2020;14:837–9.Search in Google Scholar

13. Peng, Y, Gao, Y, Zhang, X, Zhang, C, Wang, X, Zhang, H, et al.. Antidiabetic and hepatoprotective activity of the roots of Calanthe mbriata Franch. Biomed Pharmacother 2018;111:60–7. https://doi.org/10.1016/j.biopha.2018.12.066.Search in Google Scholar PubMed

14. Shamim, T, Ahmad, M, Mukhtar, M. Antihyperglycemic and hypolipidemic effects of methanolic extract of Euphorbia prostrata on alloxan induced induced diabetic rabbits. Eur Sci J 2014;10:458–65. https://doi.org/10.19044/esj.2014.v10n10p%25p.Search in Google Scholar

15. Salehi, B, Iriti, M, Vitalini, S, Antolak, Pawlikowska, Kregiel, et al.. Euphorbia-Derived natural products with potential for use in health maintenance. Biomolecules 2019;9:337–52. https://doi.org/10.3390/biom9080337.Search in Google Scholar PubMed PubMed Central

16. Alabri, THA, Al Musalami, AHS, Hossain, MA, Weli, AM, Al-Riyami, Q. Comparative study of phytochemical screening, antioxidant and antimicrobial capacities of fresh and dry leaves crude plant extracts of Datura metel L. J King Saud Univ Sci 2014;26:237–43. https://doi.org/10.1016/j.jksus.2013.07.002.Search in Google Scholar

17. Khamees, AH, Fawzi, HA, Sahib, HB. Phytochemical investigation and assessment of the hypoglycemic activity of two herbal extracts from selected Iraqi medicinal plants in alloxan-stimulated diabetic rats: a comparative study. F1000Res 2020;9:247–58. https://doi.org/10.12688/f1000research.22788.1.Search in Google Scholar

18. Park, SJ, Kim, SD, Kwag, EB, Park, JH, Yoo, HS. Acute and subchronic toxicological evaluation of the herbal product HAD-B1 in rats. Evid Based Complement Alternat Med 2021;2021:1–13. https://doi.org/10.1155/2021/9970822.Search in Google Scholar PubMed PubMed Central

19. Jaiswal, YS, Tatke, PA, Gabhe, SY, Vaidya, A. Antidiabetic activity of extracts of Anacardium occidentale Linn. leaves on n-streptozotocin diabetic rats. J. Tradit. Complement. Med. 2017;7:421–7. https://doi.org/10.1016/j.jtcme.2016.11.007.Search in Google Scholar PubMed PubMed Central

20. Yusuf, H, Yusni, Y, Meutia, F, Fahriani, M. Pharmacological evaluation of antidiabetic activity of Chromolaena odorata leaves extract in streptozotocin- induced rats. Sys Rev Pharm 2020;11:772–8. https://doi.org/10.31838/srp.2020.10.115.Search in Google Scholar

21. Naik, A, Adeyemi, SB, Vyas, B, Krishnamurthy, R. Effect of co-administration of metformin and extracts of Costus pictus D.Don leaves on alloxan-induced diabetes in rats. J Tradit Complement Med 2021. https://doi.org/10.1016/j.jtcme.2021.08.007.Search in Google Scholar PubMed PubMed Central

22. Nam, SJ, Han, YJ, Lee, W, Kang, B, Choi, MK, Han, YH, et al.. Effect of red ginseng extract on the pharmacokinetics and efficacy of metformin in streptozotocin-induced diabetic rats. Pharmaceutics 2018;10:80–90. https://doi.org/10.3390/pharmaceutics10030080.Search in Google Scholar PubMed PubMed Central

23. Shi, Y, Lu, A, Wang, X, Belhadj, Z, Wang, J, Zhang, Q. A review of existing strategies for designing longacting parenteral formulations: focus on underlying mechanisms, and future perspectives. Acta Pharm Sin B 2021;11:2396–415. https://doi.org/10.1016/j.apsb.2021.05.002.Search in Google Scholar PubMed PubMed Central

Received: 2022-08-24

Revised: 2022-12-03

Accepted: 2023-01-03

Published Online: 2023-01-20

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Articles in the same Issue

https://doi.org/10.1515/jcim-2022-0315

Keywords for this article

Euphorbia prostrata; glibenclamide; gliclazide; herb-drug interaction; medicinal herbs; metformin