Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages

Malahat Gahramanova; Andriy Ostapchuk; Olga Molozhava; Vitalina Svyatetska; Mariia Rudyk; Yevheniia Hurmach; Oleksandr Gorbach; Larysa Skivka

doi:10.1515/jcim-2020-0098

Article

Anti-inflammatory effect of polyherbal composition with hepatoprotective and choleretic properties on LPS-stimulated murine macrophages

Malahat Gahramanova , Andriy Ostapchuk , Olga Molozhava , Vitalina Svyatetska , Mariia Rudyk , Yevheniia Hurmach , Oleksandr Gorbach and Larysa Skivka

Published/Copyright: August 26, 2022

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From the journal Journal of Complementary and Integrative Medicine Volume 20 Issue 2

Abstract

Objectives

A polyherbal formulation with hepatoprotective and choleretic properties combining pharmacological potential of eight medicinal plants was developed in Nargiz Medical center (Republic of Azerbaijan) for the use as herbal tea. To explore the effect of polyherbal composition on the metabolism of LPS-stimulated macrophages in vitro.

Methods

The qualitative and quantitative phytochemical analysis was conducted using specific color reactions and gas chromatography-mass spectrometry (GC–MS). Nitric oxide (NO) assay was determined using the Griess reaction. Reactive oxygen species (ROS) generation was measured using ROS-sensitive fluorescence indicator, H2DCFDA, by flow cytometry. Arginase activity was examined by colorimetric method.

Results

The studied polyherbal formulation exerted anti-inflammatory activity in LPS-stimulated macrophages which was evidenced by dose-dependent decrease of ROS generation and by shift of arginine metabolism to the increase of arginase activity and decrease of NO release.

Conclusions

Our findings suggest that the herbal tea reduces macrophage inflammatory activity, that provide an important rationale to utilize it for the attenuation of chronic inflammation typical of hepatobiliary disorders.

Keywords: anti-inflammatory effect; hepatobiliary disorders; macrophages; phytochemical analysis; polyherbal tea

Corresponding author: Mariia Rudyk, PhD, associate professor, Microbiology and Immunology Department, ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, 2, Akademika Hlushkova Avenue, Kyiv 01601, Ukraine, Phone: +380504438775 E-mail: rosiente@gmail.com

Acknowledgments

Authors acknowledge the technical support of the D.K. Zabolotny Institute of Microbiology and Virology of the NASU.

Research funding: This work was supported by the Ministry of Education and Science of Ukraine under Grant [number 0116U002527]. The funding organization played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Author contributions: Gahramanova Malahat: Methodology, Investigation, Resources. Ostapchuk Andriy: Investigation, Resources. Molozhava Olga: Investigation. Svyatetska Vitalina: Investigation. Rudyk Mariia: Formal analysis, Investigation, Editing. Hurmach Yevheniia: Investigation. Gorbach Oleksandr: Investigation, Resources. Skivka Larysa: Conceptualization, Writing – Original Draft, Visualization, Supervision, Project administration. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Not applicable.
Ethical approval: The animal protocol was approved by ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, according to Animal Welfare Act guidelines (Minutes of the meeting of the Commission on Bioethics of ESC “Institute of Biology and Medicine”, # 4 from 10.10.2018). The study was conducted in compliance with the standards of the Convention on Bioethics of the Council of Europe’s “European Convention for the Protection of Vertebrate Animals, used for experimental and other scientific purposes” (1997).

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Received: 2020-04-13

Accepted: 2022-08-01

Published Online: 2022-08-26

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Articles in the same Issue

https://doi.org/10.1515/jcim-2020-0098

Keywords for this article

anti-inflammatory effect; hepatobiliary disorders; macrophages; phytochemical analysis; polyherbal tea