Startseite Comparative HPTLC fingerprinting profile and GC-MS analysis of raw and purified Guggulu (Commiphora wightii. Arn. Bhand) by Ayurvedic purification method
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Comparative HPTLC fingerprinting profile and GC-MS analysis of raw and purified Guggulu (Commiphora wightii. Arn. Bhand) by Ayurvedic purification method

  • Indu M. Menon ORCID logo EMAIL logo , Jollykutty Eapen und Deepa M. S.
Veröffentlicht/Copyright: 14. Mai 2021
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 19 Heft 1

Abstract

Background

Guggulu is an oleo gum resin obtained from the plant Commiphora wightii (Arn.) Bhand., used in Ayurved ic medicines for various ailments like anti-inflammatory conditions, hyperlipidemia, thyroid disorders etc. Guggulsterones E & Z are responsible for these broad ranges of pharmacological actions. It is recommended to do Shodhana (purification) before incorporating it into medicinal formulations. Sahasrayoga, an Ayurvedic text, emphasizes the purification of Guggulu in a particular media, which is a long run practice in Kerala.

Objectives

To compare the physicochemical and phytochemical parameters, quantitative estimation of Guggulsterone E & Z using high performance thin layer chromatography (HPTLC) and qualitative gas chromatography-mass spectrometry (GC-MS) analysis of Guggulu before and after purification.

Methods

Shodhana of Guggulu was performed in Water boiled with crushed fresh leaves of Neem (Azadirachta indica) and fresh rhizome of turmeric (Curcuma longa) using a special equipment (Dolayantra). Preliminary physicochemical and phytochemical evaluation, quantification of Guggulsterones E & Z using HPTLC and GC-MS analysis of raw and purified Guggulu were performed.

Results

Phytochemical evaluation of metabolites revealed marked variations. The mean concentrations of Guggulsterone E & Z showed significant differences before and after purification (p<0.01). On GC-MS analysis, it was found that few new compounds were added in the purified Guggulu.

Conclusions

Therapeutic efficacy of Guggulu might have enhanced after traditional purification.

Keywords: Ayurveda ; GC-MS; Guggulsterones E & Z; Guggulu ; HPTLC fingerprinting; shodhana
Corresponding author: Indu M. Menon, PG Scholar, Department of Dravyaguna Vijnanam, Government Ayurveda College, Thiruvananthapuram, Kerala, India, E-mail:

Funding source: Kerala State Council for Science, Technology and Environment

Award Identifier / Grant number: 00179

Ackowledgment

The authors wholeheartedly thank all the faculties of Department of Dravyaguna Vijnanam and Drug standardisation Unit, Government Ayurveda College, Thiruvananthapuram, Kerala to carry out the research.

  1. Research funding: Kerala State Council for science, Technology and environment (KSCSTE).

  2. Competing interests: There is no conflict of interest declared.

References

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/jcim-2020-0347).

Received: 2020-09-02
Accepted: 2021-01-05
Published Online: 2021-05-14

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Research Articles
  3. Comparative HPTLC fingerprinting profile and GC-MS analysis of raw and purified Guggulu (Commiphora wightii. Arn. Bhand) by Ayurvedic purification method
  4. Acute and subacute toxicity assessment of Madhulai Manappagu (Siddha herbal syrup formulation) in animal model
  5. Ethyl acetate fraction of Spathodea campanulata (Bignoniaceae) attenuates lead acetate induced testicular toxicity in male Wistar rats
  6. Comparative evaluation of cardioprotective activity of Gala and Fuji apple juice against isoprenaline-induced cardiotoxicity in rats
  7. Ameliorative effect of Kolaviron, an extract of Garcinia kola seeds, on induced hypertension
  8. Review Articles
  9. Herbal foodstuffs in Avicenna’s recommended diet to improve sperm quality and increase male fertility; an evidence-based approach
  10. Transmission modes of COVID-19 disease pandemic in the light of ancient wisdom of Ayurveda medicine: a review
  11. Burning mouth syndrome: a review of therapeutic approach
  12. Research Articles
  13. Identification of common features within massage therapists’ professional identity
  14. Psychometric validation of the modified Naranjo algorithm used in homeopathy for chronic cases
  15. An overview of the comparison of inhalation aromatherapy on emotional distress of female and male patients in preoperative period
  16. Comparing the effectiveness of mother’s live lullaby and recorded lullaby on physiological responses and sleep of preterm infants: a clinical trial study
  17. The effect of hypnotherapy on exam anxiety among nursing students
  18. The effect of abdominal massage (I Love You method) on the gastric function of preterm infants hospitalized in the neonatal intensive care units: a randomized clinical trial
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https://doi.org/10.1515/jcim-2020-0347
Schlagwörter für diesen Artikel
Ayurveda; GC-MS; Guggulsterones E & Z; Guggulu; HPTLC fingerprinting; shodhana

Artikel in diesem Heft

  1. Frontmatter
  2. Research Articles
  3. Comparative HPTLC fingerprinting profile and GC-MS analysis of raw and purified Guggulu (Commiphora wightii. Arn. Bhand) by Ayurvedic purification method
  4. Acute and subacute toxicity assessment of Madhulai Manappagu (Siddha herbal syrup formulation) in animal model
  5. Ethyl acetate fraction of Spathodea campanulata (Bignoniaceae) attenuates lead acetate induced testicular toxicity in male Wistar rats
  6. Comparative evaluation of cardioprotective activity of Gala and Fuji apple juice against isoprenaline-induced cardiotoxicity in rats
  7. Ameliorative effect of Kolaviron, an extract of Garcinia kola seeds, on induced hypertension
  8. Review Articles
  9. Herbal foodstuffs in Avicenna’s recommended diet to improve sperm quality and increase male fertility; an evidence-based approach
  10. Transmission modes of COVID-19 disease pandemic in the light of ancient wisdom of Ayurveda medicine: a review
  11. Burning mouth syndrome: a review of therapeutic approach
  12. Research Articles
  13. Identification of common features within massage therapists’ professional identity
  14. Psychometric validation of the modified Naranjo algorithm used in homeopathy for chronic cases
  15. An overview of the comparison of inhalation aromatherapy on emotional distress of female and male patients in preoperative period
  16. Comparing the effectiveness of mother’s live lullaby and recorded lullaby on physiological responses and sleep of preterm infants: a clinical trial study
  17. The effect of hypnotherapy on exam anxiety among nursing students
  18. The effect of abdominal massage (I Love You method) on the gastric function of preterm infants hospitalized in the neonatal intensive care units: a randomized clinical trial
  19. Effect of Arjun Chāl on cardiovascular risk factors – a randomized controlled clinical trial
  20. Case Report
  21. Ayurvedic management of female infertility due to tubal blockage
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