In vivo analgesic, antipyretic and anti-inflammatory potential of leaf extracts and fractions of Eria javanica
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N. M. Mahmudul Alam Bhuiya
Abstract
Background
The objective of the study was to evaluate the antinociceptive, antipyretic and anti-inflammatory activities of ethanolic extract, methanolic extract and n-hexane and chloroform-soluble fractions of methanolic extract of Eria javanica leaves in animal model (rat and mice).
Methods
The anti-nociceptive potentials of the extracts were studied using the acetic acid-induced writhing test in mice and the antipyretic activity was investigated using yeast-induced pyrexia in rats. Anti-inflammatory activity test was done on rats at a dose by using carrageenan-induced paw edema test.
Results
In acetic acid-induced writhing inhibition study in Swiss albino mice, the crude methanolic extract at 200 mg/kg and 400 mg/kg doses and the n-hexane soluble fraction of crude methanolic extract at 400 mg/kg showed statistically significant activity with 53.21 % (p<0.001), 50.36 % (p<0.001) and 67.86 % (p<0.001) inhibition respectively compared to control. The crude ethanolic extract showed statistically significant antipyretic activity from 1 hours and onwards after administration at doses of 200 mg/kg body weight (p<0.005 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively) and 400 mg/kg body weight (p<0.05 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively). The crude methanolic extract showed statistically significant antipyretic activity from 2 hours and onwards at 400 mg/kg body weight (p<0.05 at 2nd hour and p<0.001 at 3rd and 4th hour respectively) and 200 mg/kg body weight dose showed statistically significant antipyretic activity from 3 hours and onward(p<0.001) in Brewer’s yeast-induced pyrexia test in albino Wister rats. In carrageenan-induced rat’s paw edema test, crude methanolic extract showed statistically significant anti-inflammatory activity from 2nd hour and onwards. The chloroform-soluble fraction of methanolic extract also showed significant activity from 1st hour onwards.
Conclusions
This study thereby indicates that leaves of E. javanica possess peripheral analgesic, antipyretic and anti-inflammatory activities and therefore a suitable candidate for further study.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. Authors NMMAL and MH collected the plant leaves and prepared the extracts and fractions. NMMAL, MH, FA, MSHK and MMNU carried out the study design, performed the experiments, data collection, data interpretation, manuscript preparation, statistical analysis. NQ and ZAM designed and planned the studies, supervised the experiments. Author MMNU acted as correspondence.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Articles in the same Issue
- Review
- Herbal approach in the treatment of pancytopenia
- Medical Education
- Attitudes, skill and use of evidence-based practice among US Western herbal medicine providers: a national survey
- Naturopaths in Ontario, Canada: geographic patterns in intermediately-sized metropolitan areas and integration implications
- Preclinical Studies
- Effects of luteolin and luteolin-morphine co-administration on acute and chronic pain and sciatic nerve ligated-induced neuropathy in mice
- In vivo analgesic, antipyretic and anti-inflammatory potential of leaf extracts and fractions of Eria javanica
- Modulatory effects of melatonin and vitamin C on oxidative stress-mediated haemolytic anaemia and associated cardiovascular dysfunctions in rats
- Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition
- Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice
- Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats
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- Acupuncture decreases competitive anxiety prior to a competition in young athletes: a randomized controlled trial pilot study
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