Home Medicine Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats
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Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats

  • Francine Tankeu Nzufo , Constant Anatole Pieme EMAIL logo , Jacques Romain Njimou , Prosper Cabral Biapa Nya , Bruno Moukette Moukette , Bravi Marco , Chianese Angelo and Ngogang Jeanne Yonkeu
Published/Copyright: February 16, 2017

Abstract

Background

The present study focused on the antioxidant, phenolic profile and free radical scavenging-mediated protective effect of leaves extracts of Syzygium guineense var. macrocarpum against ferric nitriloacetate-induced stress in the liver, heart, kidney and brain tissues of Wistar rats homogenates.

Methods

Spectrophotometric standardized methods were used to determine the free radical scavenging potential, antioxidant and protective properties of plant extracts on rat homogenates.

Results

All the extracts showed a concentration-dependent free radical quenching potential, and the ability to protect all the tested organs by inhibiting the lipid peroxidation and potentiating or restoring the activity of enzymatic and non enzymatic markers. The polyphenolic profile revealed the presence of at least one simple phenolic acid (gallic, caffeic, para-coumaric acid) although the majority (6 out of 14) of the compounds used as standard are present in the aqueous and aqueous–ethanol extracts.

Conclusions

Ethanolic extract of leaves of S. guineense var macrocarpum (SGETOH) exhibited the highest phenol content and appeared as the best extract taking into consideration the antioxidant and organo-protective activities tested.

Acknowledgments

We are grateful to Mr. Ngansop Eric from the Cameroon National Herbarium who harvested and identified the plant material.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. FTN conducted the study assisted by MMB; BNPC conducted the statistical analysis and PCA helped in building and correcting the manuscript, PCA designed the research co-directed the research work with NYJ and provided reagents. NJR carried the HPLC of the extracts under the supervision of AC and MB.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2016-10-4
Accepted: 2016-8-20
Published Online: 2017-2-16

© 2017 Walter de Gruyter GmbH, Berlin/Boston

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