Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats
-
Francine Tankeu Nzufo
, Constant Anatole Pieme, Jacques Romain Njimou
, Prosper Cabral Biapa Nya , Bruno Moukette Moukette , Bravi Marco , Chianese Angelo and Ngogang Jeanne Yonkeu
Abstract
Background
The present study focused on the antioxidant, phenolic profile and free radical scavenging-mediated protective effect of leaves extracts of Syzygium guineense var. macrocarpum against ferric nitriloacetate-induced stress in the liver, heart, kidney and brain tissues of Wistar rats homogenates.
Methods
Spectrophotometric standardized methods were used to determine the free radical scavenging potential, antioxidant and protective properties of plant extracts on rat homogenates.
Results
All the extracts showed a concentration-dependent free radical quenching potential, and the ability to protect all the tested organs by inhibiting the lipid peroxidation and potentiating or restoring the activity of enzymatic and non enzymatic markers. The polyphenolic profile revealed the presence of at least one simple phenolic acid (gallic, caffeic, para-coumaric acid) although the majority (6 out of 14) of the compounds used as standard are present in the aqueous and aqueous–ethanol extracts.
Conclusions
Ethanolic extract of leaves of S. guineense var macrocarpum (SGETOH) exhibited the highest phenol content and appeared as the best extract taking into consideration the antioxidant and organo-protective activities tested.
Acknowledgments
We are grateful to Mr. Ngansop Eric from the Cameroon National Herbarium who harvested and identified the plant material.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission. FTN conducted the study assisted by MMB; BNPC conducted the statistical analysis and PCA helped in building and correcting the manuscript, PCA designed the research co-directed the research work with NYJ and provided reagents. NJR carried the HPLC of the extracts under the supervision of AC and MB.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Nishikawa T, Edelstein D, Du XL, Yamagishi S, Matsumura T, Kaneda Y, et al et al. Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage. Nature 2000;404:787–790.10.1038/35008121Search in Google Scholar PubMed
2. Sosa V, Moliné T, Somoza R, Paciucci R, Kondoh H, LLeonart M. Oxidative stress and cancer: an overview. Ageing Res Rev 2013;12:376–390.10.1016/j.arr.2012.10.004Search in Google Scholar PubMed
3. Al-Omar MA, Beedham C, Alsarra IA. Pathological roles of reactive oxygen species and their defense mechanisms. Saudi Pharm J 2004;12:1–17.Search in Google Scholar
4. Reuter S, Gupta SC, Madan MC, Bharat BA. Oxidative stress, inflammation, and cancer: how are they linked? Free Radic Biol Med 2010;49:1603–1616.10.1016/j.freeradbiomed.2010.09.006Search in Google Scholar PubMed PubMed Central
5. Seifried DE, Anderson EI, Fisher JA, Milner A. Review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 2007; 18:567–579.10.1016/j.jnutbio.2006.10.007Search in Google Scholar PubMed
6. Strayo D, Adhikari S, Tilak-Jain J, Menon VP, Devasagayam TP. Antioxidant activity of an aminothiazole compound: possible mechanisms. Chem Biol Interact 2008;173:215–223.10.1016/j.cbi.2008.03.011Search in Google Scholar PubMed
7. Cole GM, Lim GP, Yang F, Teter B, Begum A, Ma Q, et al et al. Prevention of Alzheimer’s disease: omega-3 fatty acid and phenolic anti-oxidant interventions. Neurobiol Aging 2005; 26 :133–136.10.1016/j.neurobiolaging.2005.09.005Search in Google Scholar PubMed
8. Rasmussen SE, Frederiksen H, Struntze K, Poulsen L. Dietary proanthocyanidins: occurrence, dietary intake, bioavailability, and protection against cardiovascular disease. Mol Nutr Food Res 2005; 49:159–174.10.1002/mnfr.200400082Search in Google Scholar PubMed
9. Arts IC, Hollman PC. Polyphenols and disease risk in epidemiologic studies. Am J Clin Nutr 2005; 81:317S–325S.10.1093/ajcn/81.1.317SSearch in Google Scholar PubMed
10. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D. Dietary flavonoids and cancer risk in the Zutphen Elderly Study. Nutr Cancer 1994; 22:175–184.10.1080/01635589409514342Search in Google Scholar PubMed
11. Pieme CA, Ngoupayo J, Nkoulou CH, Moukette B, Nono BL, Ama Moor VJ, et al et al. Syzyguim guineense extracts show antioxidant activities and beneficial activities on oxidative stress induced by ferric chloride in the liver homogenate. Antioxidants 2014; 3:618–635.10.3390/antiox3030618Search in Google Scholar PubMed PubMed Central
12. Rubiolo JA, Mithieux G, Vega FV. Resveratrol protects primary rat hepatocytes against oxidative stress damage. Activation of the Nrf2 transcription factor and augmented activities of antioxidant enzymes. Eur J Pharmacol 2008; 591:66–72.10.1016/j.ejphar.2008.06.067Search in Google Scholar
13. Malele R, Moshi M, Mwangi J, Achola K, Munenge R. Pharmacological properties of extracts from the stem bark of Syzygium guineense on the ileum and heart of laboratory rodents. Afr J Health Sci 1997; 4:43–45.Search in Google Scholar
14. Ashebir M, Ashenafi M. Assessment of the antibacterial activity of some traditional medicinal plants on some food-borne pathogens. Ethiop J Health Dev 1999; 13:211–216.Search in Google Scholar
15. Oketch-Rabah HA, Dossaji SF. Molluscicides of plant origin: molluscicidal activity of some Kenyan medicinal plants. South Afr J Sci 1998; 94:299–301.Search in Google Scholar
16. Djoukeng JD, Abou-Mansour E, Tabacchi R, Tapondjou AL, Boud H, Lontsi D. Antibacterial triterpenes from Syzygium guineense (Myrtaceae). J Ethnopharmacol 2005;101:283–286.10.1016/j.jep.2005.05.008Search in Google Scholar
17. Ior LD, Otimenyin SO, Umar M. Anti-inflammatory and analgesic activities of the ethanolic extract of the leaf of Syzygium guineense in rats and mice. IOSR J Pharm 2012; 2:33–36.10.9790/3013-24303336Search in Google Scholar
18. Noudogbessi JP, Yédomonhan P, Sohounhloué DC, Chalchat JC, Figuérédo G. Chemical composition of essential oil of Syzygium guineense (Willd.) DC. var. guineense (Myrtaceae) from Benin. Rec Nat Prod 2008;2:33–38.Search in Google Scholar
19. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxydant activity.Sonklanokarin. J Sci Tech 2004; 26.Search in Google Scholar
20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999; 26:1231–1237.10.1016/S0891-5849(98)00315-3Search in Google Scholar
21. Benzie F, Strain J. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant Power”: the FRAP assay. Anal Biochem 1996; 239:70–76.10.1006/abio.1996.0292Search in Google Scholar
22. Prieto P, Pineda M, Aguilar M. Spectophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem 1999; 269:337–341.10.1006/abio.1999.4019Search in Google Scholar
23. Singleton V, Draper D. The transfer of polyphenolic compuonds from grape seeds into wine. Am J Enol Vitic 1964; 15:34–40.10.5344/ajev.1964.15.1.34Search in Google Scholar
24. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64:555–559.10.1016/S0308-8146(98)00102-2Search in Google Scholar
25. Kumaran A, Karunakaran R. In vitro antioxidant activities of methanol extracts of Phyllanthus species from India. Lebensm Wiss Technol 2007; 40:344–352.10.1016/j.lwt.2005.09.011Search in Google Scholar
26. Moukette B, Pieme C, Biapa P, Njimou J, Moor V, Stoller M, et al et al. Phenolic content of Hypodaphnis zenkeri and its antioxidant effects against Fenton reactions’ mediated oxidative injuries on liver homogenate. Antioxidants 2014; 3:866–889.10.3390/antiox3040866Search in Google Scholar PubMed PubMed Central
27. Awai M, Narasaki M, Yanianoi Y, Seno S. Induction of diabetes in animals by parenteral administration of ferric nitrilotriacetate. A model of experimental hemochromatosis. Am J Pathol 1979; 95:663–673.Search in Google Scholar
28. Igbal M, Sharma MD, Zadeh HR, Hasan N, Abdulla M, Athar M. Glutathione metabolizing enzymes and oxidative stress in ferric nitriloacetate (Fe-NTA) mediated in hepatic injury. Redox rep 1996; 2:385–391.10.1080/13510002.1996.11747079Search in Google Scholar PubMed
29. Misra H, Fridovich I. Estimation of superoxide dismutase. J Biochem 1972; 247:3170–3178.10.1016/S0021-9258(19)45228-9Search in Google Scholar
30. Sinha A. Colorimetric assay of catalase. Anal Biochem 1972; 47:389–394.10.1016/0003-2697(72)90132-7Search in Google Scholar
31. Ellmann GL. Tissue sulfydryl group. Arch Biochem Biophys 1959; 82:70–77.10.1016/0003-9861(59)90090-6Search in Google Scholar
32. Butkovic V, Klasinc L, Bors W. Kinetic study of flavonoid reactions with stable radicals. J Agric Food Chem 2004; 52:2816–2820.10.1021/jf049880hSearch in Google Scholar
33. Miller NJ, Diplock AT, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci 1993; 84:407–412.10.1042/cs0840407Search in Google Scholar
34. Huang D, Ou B, Prior R. The chemistry behind antioxidant capacity assays. J Agr Food Chem 2005; 53:1841–1856.10.1021/jf030723cSearch in Google Scholar
35. Popovici C, Saykova Tylkowski B. Evaluation de l’activité antioxydant des composés phénoliques par la réactivité avec le radical libre DPPH. Rev Génie Ind 2009; 4:25–39.Search in Google Scholar
36. Dai J, Mumper J. Plant phenolics: extraction, analysis and their antioxidant and anticancer. Properties Mol 2010; 15:7313–7352.10.3390/molecules15107313Search in Google Scholar
37. Bossokpi IP. Etudes des activités biologiques de Fagara zanthoxyloïdes Lam. (Rutaceae). Bamako: Université de Bamako, 2002:133 (Thesis).Search in Google Scholar
38. Sroka Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol 2003; 41:753–758.10.1016/S0278-6915(02)00329-0Search in Google Scholar
39. Sundaram S, Kanniappan GV, Kannappan P. Enzymatic and non enzymatic antioxidant activity of Tabernaemontana divaricata R. Br. against DEN and Fe-NTA induced renal damage in Wistar albino rats. J Appl Pharm Sci 2015; 5:033–037.10.7324/JAPS.2015.50506Search in Google Scholar
40. Moreli Lescoat G, Cogrel P, et al et al. Antioxidant and iron-chelating activities of the flavonoids catechin, quercetin and diosmetin on iron-loaded rat hepatocytes cultures. Biochem Pharmacol 1993;45:13–19.10.1016/0006-2952(93)90371-3Search in Google Scholar
41. Van Acker S, Bast A, Van Der Vijgh W. Structural aspects of antioxidant activity of flavonoids. Rice-Evans C, Packer L, editors. Flavonoids in health and disease. New York: Marcel Decker, 1998:221–251.10.1002/chin.199817320Search in Google Scholar
42. Costa L, Garrick J, Roquè P, Pellacani C. Mechanisms of neuroprotection by quercetin: counteracting oxidative stress and more. Oxid Med Cell Longev 2016;2016:2986796. DOI:10.1155/2016/2986796.Search in Google Scholar PubMed PubMed Central
43. Haleng J, Pincemail J, Defraigne JO, Charlier C, Chapelle JP. Le stress oxydant. Rev Med Liege 2007; 62:628–638.Search in Google Scholar
44. Schulz JB, Lindenau J, Seyfried J, Dichgans J. Glutathione, oxidative stress and neurodegeneration. Eur J Biochem 2000; 267:4904–4911.10.1046/j.1432-1327.2000.01595.xSearch in Google Scholar PubMed
45. Forbes-Hernandez TY, Gasparrini M, Afrin S, Bompadre S, Mezzetti B, Quiles JL, et al et al. The healthy effects of strawberry polyphenols: which strategy behind antioxidant capacity? Crit Rev Food Sci Nutr 2015;56:S46–S59.10.1080/10408398.2015.1051919Search in Google Scholar PubMed
© 2017 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Review
- Herbal approach in the treatment of pancytopenia
- Medical Education
- Attitudes, skill and use of evidence-based practice among US Western herbal medicine providers: a national survey
- Naturopaths in Ontario, Canada: geographic patterns in intermediately-sized metropolitan areas and integration implications
- Preclinical Studies
- Effects of luteolin and luteolin-morphine co-administration on acute and chronic pain and sciatic nerve ligated-induced neuropathy in mice
- In vivo analgesic, antipyretic and anti-inflammatory potential of leaf extracts and fractions of Eria javanica
- Modulatory effects of melatonin and vitamin C on oxidative stress-mediated haemolytic anaemia and associated cardiovascular dysfunctions in rats
- Phenolic compounds from Citrus leaves: antioxidant activity and enzymatic browning inhibition
- Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice
- Organo-protective and antioxidant properties of leaf extracts of Syzygium guineense var macrocarpum against ferric nitriloacetate-induced stress of Wistar rats
- Clinical Studies
- Acupuncture decreases competitive anxiety prior to a competition in young athletes: a randomized controlled trial pilot study
- Case Report
- Herbal and dietary supplements related to diarrhea and acute kidney injury: a case report