Startseite Hypolipidaemic and haematological evaluation of the hydromethanolic extract of Ficus glumosa stem bark in alloxan-induced diabetic rats
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Hypolipidaemic and haematological evaluation of the hydromethanolic extract of Ficus glumosa stem bark in alloxan-induced diabetic rats

  • Samuel O. Onoja EMAIL logo , Yusuf N. Omeh , Maxwell I. Ezeja und Vincent N. Chukwu
Veröffentlicht/Copyright: 7. Oktober 2014

Abstract

Background: Diabetes mellitus is associated with dyslipidaemia and haematological disorders which lead to cardiovascular complications and anaemia respectively. In this study, hydromethanolic extract of Ficus glumosa was evaluated for its hypolipidaemic and haematological effects in alloxan-induced diabetic rats.

Methods: Treatment was done orally using 62.5, 125 and 250 mg/kg of F. glumosa extract (FGE) with glibenclamide (2 mg/kg) as standard reference drug for 21 days. At the end of the treatment, blood collected through direct heart puncture was used for evaluation of serum lipid profile and determination of haematological parameters. Organosomatic index evaluation of the liver, spleen and kidney was also done using standard procedures.

Results: All doses of the extract and glibenclamide (2 mg/kg) caused dose-dependent and significant (p<0.05) reduction in serum levels of total cholesterol and low-density lipoprotein cholesterol (LDL-C) of the treated rats when compared with the control group. The extract and glibenclamide produced significant (p<0.05) increase in the serum levels of high-density lipoprotein cholesterol (HDL-C) in the treated rats. The extract (62.5 mg/kg) and glibenclamide (2 mg/kg) caused a significant (p<0.05) increase in red blood cell (RBC), haemoglobin (Hb), packed cell volume (PCV) and mean corpuscular haemoglobin concentration (MCHC) in the treated rats when compared to the negative control group. However, FGE (62.5 mg/kg) and glibenclamide (2 mg/kg) produced a significant (p<0.05) decrease in mean corpuscular volume (MCV) value of the treated rats. FGE (125 and 250 mg/kg) produced a significant (p<0.05) increase in the organosomatic index of kidney in treated rats.

Conclusions: The results suggest that F. glumosa may be of help in diabetes-induced anaemia and dyslipidaemia associated with diabetes mellitus.

References

1. OyedemiSO, AdewusiEA, AiyegoroOA, AkinpeluDA. Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic Wistar rats. Asian Pac J Trop Biomed2011:3538. DOI:10.1016/S2221-1691(11)60079-8Suche in Google Scholar

2. Abd El-BakyAA. Clinicopathological effect of camellia sinensis extract on streptozotocin-induced diabetes in rats. World J Med Sci2013;8:20511.Suche in Google Scholar

3. SavuO, TirgovisteCI, AtanasiuV, GamanL, PapacoceaR, StoianI. Increase in total antioxidant capacity of plasma despite high levels of oxidative stress in uncomplicated type 2 diabetes mellitus. J Intern Med2012;40:70916.10.1177/147323001204000235Suche in Google Scholar PubMed

4. RamadanG, El-BeihNM, Abd El-GhffarEA. Modulatory effects of black green tea aqueous extract on hyperglycaemia, hyperlipidaemia and liver dysfunction in diabetic and obese rat models. Br J Nutr2009;102:16119.10.1017/S000711450999208XSuche in Google Scholar PubMed

5. ArunGS, RameshKG. Improvement of insulin sensitivity by perindopril in spontaneously hypertensive and streptozotocin diabetic rats. Indian J Pharmacol2002;34:15664.Suche in Google Scholar

6. EzeigboII, AsuzuIU. Anti-diabetic activities of the methanol leaf extracts of Hymenocardia acida (tul.) In alloxan-induced diabetic rats. Afr J Tradit Complement Altern Med2012;9:2049.10.4314/ajtcam.v9i2.4Suche in Google Scholar PubMed PubMed Central

7. SinghS, LokeYK, FurbergCD. Thiazolidinediones and heart failure: a teleo-analysis. Diab Care2007;30:214853.10.2337/dc07-0141Suche in Google Scholar PubMed

8. TankoY, YaroAH, IsaAI, YerimaM, SalehMIA, MohammedA. Toxicological and hypoglycemic studies on the leaves of Cissampelos mucronata (Menispermaceae) on blood glucose levels of streptozotocin-induced diabetic Wistar rats. J Med Plant Res2007;2:11316.Suche in Google Scholar

9. MadubunyiII, OnojaSO, AsuzuIU. In vitro anti-oxidant and in vivo anti-diabetic potentials of the methanol extract of Ficus glumosa del (Moraceae) stem bark in alloxan-induced diabetic mice. Comp Clin Pathol2012;21:38994.10.1007/s00580-010-1103-5Suche in Google Scholar

10. OnojaSO, OmehYN, EzejaMI, OdoEC, ElenduDS. Subacute antidiabetic and in vivo antioxidant effects of methanolic extract of ficus glumosa stem bark on alloxan-induced hyperglycaemic rats. Comp Clin Pathol2014. DOI:10.1007/s00580-014-1896-8Suche in Google Scholar

11. BurkillHM. The useful plants of west tropical Africa. London: Royal Botanical Gardens Kew, 1985.Suche in Google Scholar

12. TankoY, AlladeyO, AhmedMK, MohammedA, MusaKY. The effect of methanol leaves extract of Ficus glumosa on gastrointestinal motility and on castor oil induced diarrhea in laboratory animals. J Nat Prod Plant Resour2012;2:3607.Suche in Google Scholar

13. DHHS. Guide for the care and use of laboratory animals, Institute of Laboratory Animal Resources Commission on Life Sciences, National Research Council. Washington, DC: National Academy Press, 1985.Suche in Google Scholar

14. AtawodiSE. Evaluation of the hypoglycaemic, hypolipidemic and antioxidant effects of methanolic extract of “ata-ofa” polyherbal tea (A-polyherbal) in alloxan-induced diabetic rats. Drug Invention Today2011;3:2706.Suche in Google Scholar

15. ColesEH. Determination of packed cell volume. In: ColesEHW, editor. Veterinary clinical pathology. Philadelphia, PA: W.B. Saunders Co, 1986:1719.Suche in Google Scholar

16. SchalmOW, JainNC, CarrollEJ. Veterinary haematology, 3rd ed. Philadelphia, PA: Lea and Fibiger, 1975.Suche in Google Scholar

17. AllainCC, PoonLS, ChanCS, RichmondW, FuPC. Enzymatic determination of total serum cholesterol. Clin Chem1974;20:4705.10.1093/clinchem/20.4.470Suche in Google Scholar

18. BergmenyerHU, editor. Methods of enzymatic analysis, 3rd ed., vol. 8. New York: John Wiley & Sons, 1985:15460.Suche in Google Scholar

19. FootsatiS, PrincipeL. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin Chem1982;28:207780.10.1093/clinchem/28.10.2077Suche in Google Scholar

20. FriedewaldVT, LevyRI, FredricksonDS. Estimation of low density lipoprotein cholesterol in plasma, without use of preparative centrifuge. Clin Chem1972;18:499502.10.1093/clinchem/18.6.499Suche in Google Scholar

21. AlamgeerM, MuhammadNM, SajidB, et al. Hypoglycemic and hematological effects of aqueous extract of Thymus serpyllum Linn. in alloxan-induced diabetic rabbits. Afr J Pharm Pharmacol2012;6:284550.10.5897/AJPP12.1343Suche in Google Scholar

22. LenzenS, MundayR. Thiol-group reactivity, hydrophilicity and stability of alloxan, its reduction products and its N-methyl derivatives and a comparison with ninhydrin. Biochem Pharmacol1991;42:138591.10.1016/0006-2952(91)90449-FSuche in Google Scholar PubMed

23. JuJB, KimJS, ChoiCW, LeeHK, OhT, KimSC. Comparison between ethanolic and aqueous extracts from Chinese juniper berries for hypoglycaemic and hypolipidemic effects in alloxan-induced diabetic rats. J Ethnopharmacol2008;115:11015.10.1016/j.jep.2007.09.012Suche in Google Scholar PubMed

24. SharmaSR, DwivediSK, SwaruoD. Hypoglycaemic, antihyperglycaemic and hypolipidemic activities of caesalpinia bonducella seeds in rats. J Ethnopharmacol1997;58:3944.10.1016/S0378-8741(97)00079-2Suche in Google Scholar

25. NaikSR, FilhoJM, DhuleyJN, DeshmukhA. Probable mechanism of hypoglycaemic activity of basic acid, a natural product isolated from Bumelia sartorum. J Ethnopharmacol1991;33:3744.10.1016/0378-8741(91)90158-ASuche in Google Scholar

26. BrunetonJ. Pharmacognosy, phytochemistry, medicinal plants, 2nd ed., translated by HattonCK. Paris: Lavoisier Publishing, 1995:55.Suche in Google Scholar

27. DeckerEA. The role of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinone as nonessential dietary antioxidants. Nutr Rev1995;53:4956.10.1111/j.1753-4887.1995.tb01502.xSuche in Google Scholar PubMed

28. AljamalA, IbrahimA, Al-fararjehMA, AlqadiT. Effects of rosemary on lipid profile diabetic rats. Afr J Plant Sci2012;6:2225.10.5897/AJPS11.138Suche in Google Scholar

29. HalimEM, AliH. Hypoglycaemic, hypolipidemic and antioxidant properties of combination of Curcumin from cucuma longa (Linn) and partially purified product from Abrona aagusta (Linne) in STZ induced diabetes. Indian J Clin Biochem1996;17:3343.10.1007/BF02867969Suche in Google Scholar PubMed PubMed Central

30. KennedyL, BaynesJW. Non-enzymatic glycosylation and the chronic complication of diabetes: an overview. Diabetologia1984;24:938.Suche in Google Scholar

31. EzejaMI, AnagaAO, AsuzuIU. Effect of Gouania longipetala (Hemsl.) Methanolic leaf extract on the kidney and hematology of alloxan-induced hyperglycemic Wistar rats. Comp Clin Pathol2014. DOI:10.1007/s00580-014-1901-2Suche in Google Scholar

32. CrouchRK, GandySE, KimseyG, RichardA, GalbrathAG, GalbrathMP. The inhibition of islets superoxide dismutase by diabetogeric drugs. Diabetes1981;30:23541.10.2337/diab.30.3.235Suche in Google Scholar PubMed

33. SheelaCG, AugustiKT. Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic (Allium sativum, Linn). Indian J Exp Biol1992;30:5236.Suche in Google Scholar

34. AjagbonnaOP, OnifadeKI, SuleimanU. Haematological and biochemical changes in rats given extract of Calotropis procera. Sokoto J Vet Sci1999;1:3642.Suche in Google Scholar

35. GrossJL, De AzevedoMJ, SilverioSP, CananiLH, CaramoriML, ZelmanovitzT. Diabetic nephropathy: diagnosis, prevention and treatment. Diab Care2005;28:16476.10.2337/diacare.28.1.164Suche in Google Scholar

Received: 2014-5-12
Accepted: 2014-8-7
Published Online: 2014-10-7
Published in Print: 2014-12-1

©2014 by De Gruyter

Heruntergeladen am 13.10.2025 von https://www.degruyterbrill.com/document/doi/10.1515/jcim-2014-0029/html?lang=de
Button zum nach oben scrollen